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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 9

Zhengella mangrovi gen. nov., sp. nov., a novel member of family Phyllobacteriaceae isolated from mangrove sediment Free

Abstract

A Gram-negative strain, designed X9-2-2, was isolated from mangrove sediment in Yunxiao Mangrove National Nature Reserve, China. Strain X9-2-2 showed less than 96.2 % 16S rRNA gene sequence similarity to type strains of species with validly published names. Phylogenetic analysis based on 16S rRNA gene sequences and rpoB protein sequences revealed that strain X9-2-2 formed a distinct monophyletic clade within the family Phyllobacteriaceae and clustered distantly with the genera Aliihoeflea , Phyllobacterium and Hoeflea . Cells of X9-2-2 were rod-shaped, motile with subpolar or lateral flagella and facultative anaerobic. Optimal growth occurred at 30–37 °C, at pH 7 and in the presence of 2 % NaCl. The DNA G+C content of strain X9-2-2 was 64.9 mol%. The major fatty acids were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c 56.0 %), iso -C17 : 0 (9.1 %) and C12 : 0 (6.6 %). The predominant respiratory quinone was ubiquinone-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified aminolipid and two unidentified polar lipids. According to its morphology, physiology, fatty acid composition and 16S rRNA gene signature nucleotide patterns, strain X9-2-2 represents a novel species of a novel genus in the family Phyllobacteriaceae , for which the name Zhengella mangrovi gen. nov., sp. nov. is proposed. The type strain is X9-2-2 (=MCCC 1K03307=JCM 32107).

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  • Accepted:
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Keyword(s): mangrove sediment and Zhengella mangrovi gen. nov., sp. nov.
© 2018 IUMS
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2018-07-20
2024-04-18
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References

  1. Mergaert J, Swings J. Family IV. Phyllobacteriaceae fam. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology vol. 2 (The Proteobacteria), Part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria New York: Springer; 2005 p. 393
     [Google Scholar]
  2. Uchino Y, Hirata A, Yokota A, Sugiyama J. Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 1998; 44:201–210[PubMed]
     [Google Scholar]
  3. Roh SW, Kim KH, Nam YD, Chang HW, Kim MS et al. Aliihoeflea aestuarii gen. nov., sp. nov., a novel bacterium isolated from tidal flat sediment. J Microbiol 2008; 46:594–598 [View Article][PubMed]
     [Google Scholar]
  4. Kampfer P, Neef A, Salkinoja-Salonen MS, Buss HJ. Chelatobacter heintzii (Auling et al. 1993) is a later subjective synonym of Aminobacter aminovorans (Urakami et al. 1992). Int J Syst Evol Microbiol 2002; 52:835–839 [View Article][PubMed]
     [Google Scholar]
  5. Bambauer A, Rainey FA, Stackebrandt E, Winter J. Characterization of Aquamicrobium defluvii gen. nov. sp. nov., a thiophene-2-carboxylate-metabolizing bacterium from activated sludge. Arch Microbiol 1998; 169:293–302[PubMed]
     [Google Scholar]
  6. Doronina NV, Kaparullina EN, Trotsenko YA, Nörtemann B, Bucheli-Witschel M et al. Chelativorans multitrophicus gen. nov., sp. nov. and Chelativorans oligotrophicus sp. nov., aerobic EDTA-degrading bacteria. Int J Syst Evol Microbiol 2010; 60:1044–1051 [View Article][PubMed]
     [Google Scholar]
  7. Li Y, Guo LM, Chang JP, Xie SJ, Piao CG et al. Corticibacterium populi gen. nov., sp. nov., a member of the family Phyllobacteriaceae, isolated from bark of Populus×euramericana. Int J Syst Evol Microbiol 2016; 66:2617–2622 [View Article][PubMed]
     [Google Scholar]
  8. Peix A, Rivas R, Trujillo ME, Vancanneyt M, Velázquez E et al. Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov. Int J Syst Evol Microbiol 2005; 55:1163–1166 [View Article][PubMed]
     [Google Scholar]
  9. Park S, Lee JS, Lee KC, Yoon JH. Lentilitoribacter donghaensis gen. nov., sp. nov., a slowly-growing alphaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek 2013; 103:457–464 [View Article][PubMed]
     [Google Scholar]
  10. Jarvis BDW, van Berkum P, Chen WX, Nour SM, Fernandez MP et al. Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 1997; 47:895–898
     [Google Scholar]
  11. Labbé N, Parent S, Villemur R. Nitratireductor aquibiodomus gen. nov., sp. nov., a novel alpha-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol 2004; 54:269–273 [View Article][PubMed]
     [Google Scholar]
  12. Hameed A, Shahina M, Lai WA, Lin SY, Young LS et al. Oricola cellulosilytica gen. nov., sp. nov., a cellulose-degrading bacterium of the family Phyllobacteriaceae isolated from surface seashore water, and emended descriptions of Mesorhizobium loti and Phyllobacterium myrsinacearum. Antonie van Leeuwenhoek 2015; 107:759–771 [View Article][PubMed]
     [Google Scholar]
  13. Knösel D. Prüfung von bakterien auf Fähigkeit zur Sternbildung. Zentralbl Bakteriol Parasitenk Infecktionskr Hyg Abt 1962; 2:79–100
     [Google Scholar]
  14. Kämpfer P, Müller C, Mau M, Neef A, Auling G et al. Description of Pseudaminobacter gen. nov. with two new species, Pseudaminobacter salicylatoxidans sp. nov. and Pseudaminobacter defluvii sp. nov. Int J Syst Bacteriol 1999; 49:887–897 [View Article][PubMed]
     [Google Scholar]
  15. Hyeon JW, Jeong SE, Baek K, Jeon CO. Roseitalea porphyridii gen. nov., sp. nov., isolated from a red alga, and reclassification of Hoeflea suaedae Chung et al. 2013 as Pseudohoeflea suaedae gen. nov., comb. nov. Int J Syst Evol Microbiol 2017; 67:362–368 [View Article][PubMed]
     [Google Scholar]
  16. Han L, Mo Y, Feng Q, Zhang R, Zhao X et al. Tianweitania sediminis gen. nov., sp. nov., a member of the family Phyllobacteriaceae, isolated from subsurface sediment core. Int J Syst Evol Microbiol 2016; 66:719–724 [View Article][PubMed]
     [Google Scholar]
  17. Lei X, Li Y, Chen Z, Zheng W, Lai Q et al. Altererythrobacter xiamenensis sp. nov., an algicidal bacterium isolated from red tide seawater. Int J Syst Evol Microbiol 2014; 64:631–637 [View Article][PubMed]
     [Google Scholar]
  18. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  19. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  20. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
     [Google Scholar]
  21. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1992; 9:945–967
     [Google Scholar]
  22. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376[PubMed]
     [Google Scholar]
  23. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
     [Google Scholar]
  24. Li R, Li Y, Kristiansen K, Wang J. SOAP: short oligonucleotide alignment program. Bioinformatics 2008; 24:713–714 [View Article][PubMed]
     [Google Scholar]
  25. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
     [Google Scholar]
  26. Liao H, Li Y, Zhang M, Lin X, Lai Q et al. Altererythrobacter mangrovi sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2017; 67:4851–4856 [View Article][PubMed]
     [Google Scholar]
  27. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  28. Kates M. Techniques of Lipidology, 2nd ed. rev. Amsterdam: Elsevier; 1986
     [Google Scholar]
  29. Collins M. Isoprenoid quinone analysis in bacterial classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985
     [Google Scholar]
  30. Lin SY, Hameed A, Young CC, Liu YC, Hsu YH et al. Thalassocola ureilytica gen. nov., sp. nov., of the family Phyllobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 2015; 65:4100–4106 [View Article][PubMed]
     [Google Scholar]
  31. Mantelin S, Saux MF, Zakhia F, Béna G, Bonneau S et al. Emended description of the genus Phyllobacterium and description of four novel species associated with plant roots: Phyllobacterium bourgognense sp. nov., Phyllobacterium ifriqiyense sp. nov., Phyllobacterium leguminum sp. nov. and Phyllobacterium brassicacearum sp. nov. Int J Syst Evol Microbiol 2006; 56:827–839 [View Article][PubMed]
     [Google Scholar]
  32. Lai Q, Yu Z, Yuan J, Sun F, Shao Z. Nitratireductor indicus sp. nov., isolated from deep-sea water. Int J Syst Evol Microbiol 2011; 61:295–298 [View Article][PubMed]
     [Google Scholar]
  33. Martínez-Hidalgo P, Ramírez-Bahena MH, Flores-Félix JD, Rivas R, Igual JM et al. Revision of the taxonomic status of type strains of Mesorhizobium loti and reclassification of strain USDA 3471T as the type strain of Mesorhizobium erdmanii sp. nov. and ATCC 33669T as the type strain of Mesorhizobium jarvisii sp. nov. Int J Syst Evol Microbiol 2015; 65:1703–1708 [View Article][PubMed]
     [Google Scholar]
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Zhengella mangrovi gen. nov., sp. nov., a novel member of family Phyllobacteriaceae isolated from mangrove sediment
Int J Syst Evol Microbiol 68, 2819 (2018); https://doi.org/10.1099/ijsem.0.002900
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