@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002933, author = "Park, Yu Jin and Jeong, Sang Eun and Jung, Hye Su and Park, So Yoon and Jeon, Che Ok", title = "Nocardioides currus sp. nov., isolated from a mobile car air-conditioning system", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "9", pages = "2977-2982", doi = "https://doi.org/10.1099/ijsem.0.002933", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002933", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "new taxa", keywords = "air-conditioning system", keywords = "Nocardioides currus", keywords = "mobile car", keywords = "taxonomy", abstract = "A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3T, was isolated from a car air-conditioning system in the Republic of Korea. Cells were non-motile rods showing catalase- and oxidase-positive reactions. Growth of IB-3T was observed at 20–40 °C (optimum, 25 °C), at pH 6.5–9.0 (optimum, pH 7.5) and in the presence of 0–1 % (w/v) NaCl (optimum, 0 %). Menaquinone-8 (H4) was detected as the predominant respiratory quinone and iso-C16 : 0, 10-methyl-C17 : 0, iso-C17 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 0, 10-methyl-C18 : 0 (TBSA) and C17 : 0 were identified as the major cellular fatty acids. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids. The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid. The G+C content of the genomic DNA was 71.5 mol%. IB-3T was most closely related to Nocardioides terrigena DS-17T with a 98.0 % 16S rRNA gene sequence similarity. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioides of the family Nocardioidaceae . On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides , for which the name Nocardioides currus sp. nov. is proposed. The type strain is IB-3T (=KACC 19522T=JCM 32672T).", }