@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002938, author = "Sakdapetsiri, Chatsuda and Ngaemthao, Wipaporn and Suriyachadkun, Chanwit and Duangmal, Kannika and Kitpreechavanich, Vichien", title = "Actinomycetospora endophytica sp. nov., isolated from wild orchid (Podochilus microphyllus Lindl.) in Thailand", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "9", pages = "3017-3021", doi = "https://doi.org/10.1099/ijsem.0.002938", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002938", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Actinomycetospora endophytica", keywords = "endophytic actinomycete", abstract = "A novel endophytic actinomycete, designated strain A-T 8314T, was isolated from a wild orchid, Podochilus microphyllus Lindl., collected from Trat Province, Thailand. The taxonomic position of strain A-T 8314T was established using a combination of genotypic and phenotypic analyses. The isolate was a Gram-positive bacterium that developed bud-like spore chains. Strain A-T 8314T grew aerobically at an optimum temperature of 20–25 °C and an optimal pH 6.0. The cell wall contained meso-diaminopimelic acid, and the whole-cell sugars were ribose, arabinose and galactose. The predominant menaquinone was MK-8 (H4). The polar lipid profile contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, phosphatidylmonomethylethanolamine, hydroxy-phosphatidylmonomethylethanolamine and hydroxyl phosphatidylethanolamine. The predominant cellular fatty acid was iso-C16 : 0. The DNA G+C content of the genomic DNA was 73.2±0.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A-T 8314T belonged to the genus Actinomycetospora , and was most closely related to Actinomycetospora chiangmaiensis YIM 0006T (98.8 %) and Actinomycetospora corticicola 014-5T (98.6 %). The DNA–DNA relatedness values that distinguished A-T 8314T from its closest species were below 70 %. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, it was concluded that the new isolate represents as a novel species, for which the name Actinomycetospora endophytica sp. nov is proposed. The type strain is A-T 8314T (=TBRC 5722T=NBRC 113235T).", }