RT Journal Article SR Electronic(1) A1 Mu, Shan A1 Sun, Tianyu A1 Li, Yuanyuan A1 Jiang, Shanwen A1 Guo, Xiaowei A1 Wang, Xiangjing A1 Zhao, Junwei A1 Xiang, WenshengYR 2018 T1 Glycomyces dulcitolivorans sp. nov., isolated from rhizosphere soil of wheat (Triticum aestivum L.) JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 9 SP 3034 OP 3039 DO https://doi.org/10.1099/ijsem.0.002948 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain SJ-25T, was isolated from rhizosphere soil of wheat (Triticumaestivum L.) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain SJ-25T belonged to the genus Glycomyces and was closely related to Glycomyces scopariae YIM 56256T (99.0 % 16S rRNA gene sequence similarity), Glycomyces artemisiae IXS4T (98.8 %), Glycomyces sambucus E71T (98.7 %) and Glycomyces mayteni YIM 61331T (98.4 %). Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain SJ-25T to the genus Glycomyces . The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates contained galactose, glucose and xylose. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, glycolipid and two unidentified polar lipids. The menaquinones were MK-11, MK-10(H4) and MK-10(H2). Major fatty acids were iso-C16 : 0, anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C14 : 0. The DNA G+C content was 72.2 mol%. The combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain SJ-25T could be distinguished from its closely related strains. Therefore, it is proposed that strain SJ-25T represents a novel species of the genus Glycomyces , for which the name Glycomyces dulcitolivorans sp. nov. is proposed. The type strain is SJ-25T (=CGMCC 4.7414T=DSM 105121T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002948