RT Journal Article SR Electronic(1) A1 Ambika Manirajan, Binoy A1 Suarez, Christian A1 Ratering, Stefan A1 Rusch, Volker A1 Geissler-Plaum, Rita A1 Cardinale, Massimiliano A1 Schnell, SylviaYR 2018 T1 Spirosoma pollinicola sp. nov., isolated from pollen of common hazel (Corylus avellana L.) JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 10 SP 3248 OP 3254 DO https://doi.org/10.1099/ijsem.0.002973 PB Microbiology Society, SN 1466-5034, AB A Gram-negative bacterium, strain HA7T, was isolated from the microhabitat of common hazel (Corylus avellana L.) pollen. HA7T was found to be an aerobic, rod-shaped, catalase-positive, oxidase-negative bacterium with an optimum growth temperature of 25 °C and pH of 7. The nearly complete 16S rRNA gene sequence of HA7T strain showed the closest similarities to Spirosoma linguale DSM 74T (97.4 %) and Spirosoma fluviale DSM 29961T (97.43 %). The major fatty acids (>5 %) were C16 : 1ω5c, summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0 and iso-C17 : 0 3-OH. The major polar lipids were an unidentified aminophospholipid and phosphatidylethanolamine. The major respiratory quinone detected was menaquinone MK-7 (95 %). The draft genome sequence included 8 794 837 bases, which contained 3665 predicted coding sequences and had a G+C content of 47.9 mol%. The genome-based comparison between HA7T and S. linguale DSM 74T and S. fluviale DSM 29961T with pairwise average nucleotide identity indicated a clear distinction, between 76.2–76.3 %. Moreover, the digital DNA–DNA relatedness of HA7T to these strains was 26.5 and 25.1 %. Based on the differential genotypic, phenotypic and chemotaxonomic properties to closely related type strains, strain HA7T ought to be assigned with the status of a new species, for which the name Spirosoma pollinicola sp. nov. is proposed. The type strain is HA7T (DSM 105799T=LMG 30282T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002973