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Volume 68, Issue 11

Blastococcus litoris sp. nov., isolated from sea-tidal flat sediment Free

Abstract

A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea. Cells were aerobic, catalase-negative, oxidase-positive, non-motile and cocci, occurring singly, in pairs or in tetrads, and often tending to form aggregates. The strain grew at 4–45 °C (optimum, 28–37 °C), at pH 4.0–11.0 (pH 7.0–9.0) and in the presence of 0–11 % (w/v) NaCl (0–3 %). Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus . The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. Whole-cell sugar analysis of strain GP-S2-8 revealed rhamnose, glucose and mannose as characteristic sugars. The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0. The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids. The DNA G+C content was 74.2 mol%. DNA–DNA relatedness values between strain GP-S2-8 and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %. On the basis of the phenotypic differences and DNA–DNA relatedness data, the isolate represents a new species of the genus Blastococcus , for which the name Blastococcus litoris sp. nov. is proposed. The type strain is GP-S2-8 (=KCCM 43275=JCM 32354=DSM 106127=KCTC 49078).

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  • Accepted:
  • Published Online:
Keyword(s): Actinobacteria , beach sediment , Blastococcus and sea-tidal flat
© 2018 IUMS
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2018-09-14
2024-04-19
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Blastococcus litoris sp. nov., isolated from sea-tidal flat sediment
Int J Syst Evol Microbiol 68, 3435 (2018); https://doi.org/10.1099/ijsem.0.003004
/content/journal/ijsem/10.1099/ijsem.0.003004
/content/journal/ijsem/10.1099/ijsem.0.003004
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