@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003012, author = "Gerritsen, Jacoline and Umanets, Alexander and Staneva, Ivelina and Hornung, Bastian and Ritari, Jarmo and Paulin, Lars and Rijkers, Ger T. and de Vos, Willem M. and Smidt, Hauke", title = "Romboutsia hominis sp. nov., the first human gut-derived representative of the genus Romboutsia, isolated from ileostoma effluent", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "11", pages = "3479-3486", doi = "https://doi.org/10.1099/ijsem.0.003012", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003012", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "human intestine", keywords = "Romboutsia", keywords = "Peptostreptococcaceae", keywords = "Clostridium", keywords = "ileostoma effluent", abstract = "A Gram-stain-positive, motile, rod-shaped, obligately anaerobic bacterium, designated FRIFIT, was isolated from human ileostoma effluent and characterized. On the basis of 16S rRNA gene sequence similarity, strain FRIFIT was most closely related to the species Romboutsia ilealis CRIBT (97.7 %), Romboutsia lituseburensis DSM 797T (97.6 %) and Romboutsia sedimentorum LAM201T (96.6 %). The level of DNA–DNA relatedness between strain FRIFIT and R. ilealis CRIBT was 13.9±3.3 % based on DNA–DNA hybridization. Whole genome sequence-based average nucleotide identity between strain FRIFIT and closely related Romboutsia strains ranged from 78.4–79.1 %. The genomic DNA G+C content of strain FRIFIT was 27.8 mol%. The major cellular fatty acids of strain FRIFIT were saturated and unsaturated straight-chain C12–C19 fatty acids as well as cyclopropane fatty acids, with C16 : 0 being the predominant fatty acid. The polar lipid profile comprised five phospholipids and six glycolipids. These results, together with differences in phenotypic features, support the proposal that strain FRIFIT represents a novel species within the genus Romboutsia , for which the name Romboutsia hominis sp. nov. is proposed. The type strain is FRIFIT (=DSM 28814T=KCTC 15553T).", }