RT Journal Article SR Electronic(1) A1 Chen, Rou-Wen A1 Wang, Ke-Xin A1 Zhou, Xue-Feng A1 Long, Chao A1 Tian, Xin-Peng A1 Long, Li-JuanYR 2018 T1 Indioceanicola profundi gen. nov., sp. nov., isolated from Indian Ocean sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 12 SP 3707 OP 3712 DO https://doi.org/10.1099/ijsem.0.003016 PB Microbiology Society, SN 1466-5034, AB A novel basophilic bacterial strain, designated as SCSIO 08040T, was recovered from a deep-sea sediment sample collected from the Indian Ocean. The strain was Gram-stain-negative, vibrioid or spiral, light pink, 0.6–1.0 µm wide and 1.0–2.5 µm long. Growth occurred at 20–45 °C, pH 7–11 and <5 % (w/v) NaCl, with optimum growth at 28–37 °C, pH 7 and 0–3 % (w/v) NaCl. Catalase-, oxidase and urease-positive, nitrate reduction-negative. Analysis of 16S rRNA gene sequencing revealed that strain SCSIO 08040T had the highest similarity of 95.3 % to Rhodocista pekingensis 3-pT. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences showed that the novel isolate formed a distinct phylogenetic lineage in the family Rhodospirillaceae . The whole-cell hydrolysate contained meso–diaminopimelic acid, galactose, mannose and xylose. The total cellular fatty acid profile was dominated by C18:1ω7c and C19:0cycloω8c. Q-10 was the predominant ubiquinone. The major phospholipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The DNA G+C content of strain SCSIO 08040T was 66.82 mol%. Based on these polyphasic data, a new genus, Indioceanicola gen. nov., is proposed in the family Rhodospirillaceae with the type species Indioceanicola profundi sp. nov. and the type strain SCSIO 08040T (=DSM 105146T=CGMCC 1.15812T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003016