%0 Journal Article %A Chen, Rou-Wen %A Wang, Ke-Xin %A Wang, Fa-Zuo %A He, Yuan-Qiu %A Long, Li-Juan %A Tian, Xin-Peng %T Rubrobacter indicoceani sp. nov., a new marine actinobacterium isolated from Indian Ocean sediment %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 11 %P 3487-3493 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003018 %K Rubrobacter indicoceani sp. nov. %K marine actinobacteria %K polyphasic taxonomy %K Rubrobacter %K Indian Ocean %I Microbiology Society, %X A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean. The strain was Gram-stain-positive, rod-shaped and salmon pink in colour. Good growth occurred on marine agar with 1–5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight. Sensitive to short ultraviolet radiation. Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter . Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter . The total cellular fatty acid profile was dominated by C16 : 0 12-methyl. MK-8 was the main menaquinone. The peptidoglycan type was A3α (l-Lys-l-Ala). The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids. Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids. Based on these polyphasic data, a new species, Rubrobacter indicoceani sp. nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003018