RT Journal Article SR Electronic(1) A1 Fu, Tianyu A1 Jia, Chao A1 Fu, Liang A1 Zhou, Shun A1 Yao, Peng A1 Du, Rui A1 Sun, Hao A1 Yang, Zuosheng A1 Shi, Xiaochong A1 Zhang, Xiao-HuaYR 2018 T1 Marinifilum breve sp. nov., a marine bacterium isolated from the Yongle Blue Hole in the South China Sea and emended description of the genus Marinifilum JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 11 SP 3540 OP 3545 DO https://doi.org/10.1099/ijsem.0.003027 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, facultative anaerobic, non-motile, short-clavate and non-flagellated marine bacterium strain, designated JC075T, was isolated from the Yongle Blue Hole in the South China Sea. Based on the 16S rRNA gene sequence, strain JC075T was found to be closely related to Marinifilum albidiflavum FB208T (97.10 %), Marinifilum flexuosum DSM 21950T (96.43 %) and Marinifilum fragile JCM 15579T (95.58 %), with less than 90.24 % sequence similarity to other genera of the family Marinifilaceae . The growth temperature was in the range of 10–37 °C, and the optimum temperature was 16 °C. Optimal growth occurred at pH 7.0 and in the presence of 3 % (w/v) NaCl. The isoprenoid quinone of strain JC075T was identified as menaquinone-7 and the predominant fatty acids (>10 %) were iso-C15 : 0 (47.9 %), summed feature 9 (C17 : 1 or/and iso-C17 : 1 ω9c; 18.7 %) and iso-C17 : 0 3-OH (14.9 %). The major polar lipids were one phosphatidylethanolamine, one phospholipid, one aminophospholipid, one glycolipid, one aminolipid and two unidentified lipids. The DNA G+C content of strain JC075T was 35.8 mol%. On the basis of polyphasic analysis, strain JC075T was considered to represent a novel species of the genus Marinifilum, for which the name Marinifilum breve sp. nov. is proposed. The type strain is JC075T (=KCTC 15646T=MCCC 1K03477T=JCM 32401T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003027