@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003036, author = "Wu, Minghan and Zong, Yuanyuan and Guo, Wei and Wang, Gejiao and Li, Mingshun", title = "Paenibacillus montanisoli sp. nov., isolated from mountain area soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "11", pages = "3569-3575", doi = "https://doi.org/10.1099/ijsem.0.003036", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003036", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Paenibacillus", keywords = "polytaxonomy", keywords = "mountain soil", keywords = "Bacillaceae", abstract = "A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T, was isolated from Dafan, Hubei Province, China. Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %). Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P. taihuensis THMBG22T (88.4 %) and P. sacheonensis DSM SY01T (85.5 %). The DNA–DNA hybridization values between strain RA17T and the two type strains, P. taihuensis THMBG22T and P. rhizoryzae ACCC 1ZS3-5T, were 36.8 and 22.9 %, respectively. Its genome size was 6.17 Mb, comprising 5677 predicted genes with a DNA G+C content of 52.82 mol %. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, four aminophospholipids, an unidentified phospholipid and an unidentified polar lipid. The major quinone was menaquinone 7. The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid. The low DNA–DNA hybridization values, physiological and biochemical differences, such as growth at 4 °C, acid production from inositol, lack of α-chymotrypsin activity, no casein hydrolysis, and negative for acid production from d-fructose, melibiose and sucrose, could distinguish strain RA17T from its closely related species. Consequently, strain RA17T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus montanisoli sp. nov. is proposed, with RA17T (=KCTC 33894T=CCTCC AB 2017053T) as the type strain.", }