@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003079, author = "Li, Chan and Shi, Kaixiang and Zhang, Yuxiao and Wang, Gejiao", title = "Nocardioides silvaticus sp. nov., isolated from forest soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "1", pages = "68-73", doi = "https://doi.org/10.1099/ijsem.0.003079", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003079", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "fatty acid", keywords = "Nocardioides", keywords = "Taxonomic Description", keywords = "polar lipids", keywords = "DNA hybridization", abstract = "A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34T was isolated from forest soil. According to 16S rRNA gene sequence analysis, strain S-34T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696T (97.3 %) and Nocardioides panacisoli Gsoil 346T (97.0 %), Nocardioides litorisoli X-2T (96.5 %) and Nocardioides immobilis FLL521T (96.4 %). Phylogenetic trees showed that strain S-34T fell within the cluster containing strain S-34T and N. immobilis FLL521T. The levels of DNA–DNA relatedness between strain S-34T and N. thalensis CCTCC AB 2016296T and between strain S-34T and N. panacisoli KCTC 19470T were 50.6 and 58.8 %, respectively. The genome orthoANI value between strain S-34T and N. immobilis CCTCC AB 2017083T was 82.4 %. Strain S-34T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1 ω8c, C17:1 ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids. The genome length of strain S-34T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%. Strain S-34T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA–DNA hybridization, polar lipids and some biochemical differences. Therefore, strain S-34T represents a novel species of the genus Nocardioides , for which the name Nocardioides silvaticus sp. nov. is proposed. The type strain is S-34T (=KCTC 49137T=CCTCC AB 2018079T).", }