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Volume 69, Issue 11

gen. nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production Free

Abstract

Two isolates of heterotrophic, facultatively anaerobic, marine bacteria, designated DM1 and DM2, were recovered from a lagoon sediment sample of Dongsha Island, Taiwan. Cells were Gram-reaction-negative rods. Nearly all of the cells were non-motile and non-flagellated during the late exponential to early stationary phase of growth, while a few of the cells exhibited motility with monotrichous flagellation. The two isolates required NaCl for growth and grew optimally at about 30 °C, 2–3 % NaCl and pH 7–8. They grew aerobically and could achieve anaerobic growth by fermenting -glucose or other carbohydrates with production of acids and the gases, including CO and H. Ubiquinone Q-8 was the only respiratory quinone. Cellular fatty acids were predominated by C, Cω7 and Cω7. The major polar lipid was phosphatidylethanolamine. Strains DM1 and DM2 had DNA G+C contents of 52.0 and 51.8 mol%, respectively, as determined by HPLC analysis. Phylogenetic analyses based on 16S rRNA gene sequences clearly indicated that the two isolates formed a distinct genus-level lineage in the family of the class and was an outgroup with respect to a stable supragenic clade comprising species of the genera , and . The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of a novel species and genus inside the family , for which the name gen. nov., sp. nov. is proposed. The type strain is DM2 (=BCRC 81069=JCM 32096).

  • Received:
  • Accepted:
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Keyword(s): Aeromonadaceae , Dongshaea marina , Gammaproteobacteria , Oceanimonas , Oceanisphaera and Zobellella
© 2019 IUMS
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2019-11-01
2024-04-25
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Dongshaea marina gen. nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production
Int J Syst Evol Microbiol 69, 3318 (2019); https://doi.org/10.1099/ijsem.0.003080
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