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Volume 69, Issue 3

Planctomonas deserti gen. nov., sp. nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert Free

Abstract

A Gram-staining-positive, aerobic, irregular coccoid- to ovoid-shaped, non-spore-forming and motile bacterium, designated strain 13S1-3, was isolated from a soil sample from the rhizosphere of Tamarix collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China. The strain was examined by a polyphasic approach to clarify its taxonomic position. Strain 13S1-3 grew optimally at 28–30 °C, pH 7.0 and with 0–1 % (w/v) NaCl. The cell-wall peptidoglycan was of the B2γ type and contained d-alanine, d-glutamic acid, glycine, d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid. Ribose, xylose, glucose and galactose were detected as cell-wall sugars. The acyl type of the muramic acid was acetyl. The predominant menaquinones were MK-12, MK-11, MK-13 and MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified phospholipid. The major whole-cell fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The DNA G+C content was 70.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that 13S1-3 represented a member of the family Microbacteriaceae and showed the highest level of 16S rRNA gene sequence similarity with Frondihabitans australicus E1HC-02 (97.11 %). Phylogenetic trees revealed that 13S1-3 formed a distinct lineage with respect to closely related genera within the family Microbacteriaceae . On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, 13S1-3 is distinguishable from phylogenetically related genera in the family Microbacteriaceae , and represents a novel species of a new genus, for which the name Planctomonas deserti gen. nov., sp. nov. is proposed. The type strain is 13S1-3 (=KCTC 49115=CGMCC 1.16554).

  • Received:
  • Accepted:
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Keyword(s): actinobacteria , Microbacteriaceae , Planctomonas deserti , strain 13S1-3T and taxonomy
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2018-11-29
2024-04-19
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Planctomonas deserti gen. nov., sp. nov., a new member of the family Microbacteriaceae isolated from soil of the Taklamakan desert
Int J Syst Evol Microbiol 69, 616 (2019); https://doi.org/10.1099/ijsem.0.003095
/content/journal/ijsem/10.1099/ijsem.0.003095
/content/journal/ijsem/10.1099/ijsem.0.003095
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