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Volume 69, Issue 2

Aquabacterium tepidiphilum sp. nov., a moderately thermophilic bacterium isolated from a hot spring Free

Abstract

A Gram-stain-negative, aerobic, non-spore-forming and rod-shaped bacterium, designated YIM 730274, was isolated from a sediment sample collected from a hot spring located in Tibet, PR China, and was characterized by using a polyphasic taxonomy approach. Cells were motile by means of a polar flagellum. The strain was oxidase- and catalase-positive, and contained polyalkanoates and polyphosphate as storage polymers. Growth occurred at 25–50 °C, at pH 6.0–8.5 and with 0.5–1.0 % NaCl. The major fatty acids (>10 %) were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The known polar lipids comprised of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine. The isoprenoid quinone was Q-8. The G+C content of genomic DNA was 70.7 mol%. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the strain forms a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Aquabacterium in the class Betaproteobacteria . The most closely related phylogenetic neighbours were Aquabacterium limnoticum ABP-4 (97.8 % 16S rRNA gene sequence identity) and Aquabacterium commune B8 (97.2 % 16SrRNA gene sequence identity). DNA–DNA relatedness values between YIM 730274 and A. limnoticum KCTC 23306 (46.4±0.4 %) and A. commune DSM 11901 (42.2±1.2 %) were well below the 70 % limit for species identification. YIM 730274 was distinguishable from other members of the genus Aquabacterium by the differences in phenotypic, chemotaxonomic and genotypic characteristics. YIM 730274 merits recognition as a representative of a novel species of the genus Aquabacterium . It is proposed that the isolate should be classified in the genus Aquabacterium as representing a novel species, Aquabacterium tepidiphilum sp. nov. The type strain is YIM 730274 (=KCTC 52716=CCTCC AB 2016295).

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Keyword(s): Aquabacterium tepidiphilum sp. nov , hot spring , polyphasic taxonomy approach , sediment sample and Tibet, China
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2018-11-07
2024-04-19
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Aquabacterium tepidiphilum sp. nov., a moderately thermophilic bacterium isolated from a hot spring
Int J Syst Evol Microbiol 69, 337 (2019); https://doi.org/10.1099/ijsem.0.003103
/content/journal/ijsem/10.1099/ijsem.0.003103
/content/journal/ijsem/10.1099/ijsem.0.003103
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