Aquabacterium tepidiphilum sp. nov., a moderately thermophilic bacterium isolated from a hot spring Khan, Inam Ullah and Habib, Neeli and Asem, Mipeshwaree Devi and Salam, Nimaichand and Xiao, Min and Zhou, En-Min and Zhi, Xiao-Yang and Li, Wen-Jun,, 69, 337-342 (2019), doi = https://doi.org/10.1099/ijsem.0.003103, publicationName = Microbiology Society, issn = 1466-5026, abstract= A Gram-stain-negative, aerobic, non-spore-forming and rod-shaped bacterium, designated YIM 730274T, was isolated from a sediment sample collected from a hot spring located in Tibet, PR China, and was characterized by using a polyphasic taxonomy approach. Cells were motile by means of a polar flagellum. The strain was oxidase- and catalase-positive, and contained polyalkanoates and polyphosphate as storage polymers. Growth occurred at 25–50 °C, at pH 6.0–8.5 and with 0.5–1.0 % NaCl. The major fatty acids (>10 %) were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The known polar lipids comprised of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine. The isoprenoid quinone was Q-8. The G+C content of genomic DNA was 70.7 mol%. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the strain forms a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Aquabacterium in the class Betaproteobacteria . The most closely related phylogenetic neighbours were Aquabacterium limnoticum ABP-4T (97.8 % 16S rRNA gene sequence identity) and Aquabacterium commune B8T (97.2 % 16SrRNA gene sequence identity). DNA–DNA relatedness values between YIM 730274T and A. limnoticum KCTC 23306T (46.4±0.4 %) and A. commune DSM 11901T (42.2±1.2 %) were well below the 70 % limit for species identification. YIM 730274T was distinguishable from other members of the genus Aquabacterium by the differences in phenotypic, chemotaxonomic and genotypic characteristics. YIM 730274T merits recognition as a representative of a novel species of the genus Aquabacterium . It is proposed that the isolate should be classified in the genus Aquabacterium as representing a novel species, Aquabacterium tepidiphilum sp. nov. The type strain is YIM 730274T (=KCTC 52716T=CCTCC AB 2016295T)., language=, type=