@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003111, author = "Phurbu, Dorji and Pema, Yangzom and Ma, Chao and Lu, Huibin and Li, Huabing and Tian, Yanling and Xing, Peng", title = "Nitrincola tibetensis sp. nov., isolated from Lake XuguoCo on the Tibetan Plateau", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "1", pages = "123-128", doi = "https://doi.org/10.1099/ijsem.0.003111", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003111", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "alkaliphilic", keywords = "genotypic and phenotypic analysis", keywords = "Nitrincola tibetensis", keywords = "Tibetan Plateau", keywords = "Lake XuguoCo", abstract = "A novel Gram-stain-negative, motile and rod-shaped bacterium, designated xg18T, was isolated from Lake XuguoCo on the Tibetan Plateau. The strain was able to grow optimally at 0–2 % NaCl and tolerate up to 6 % NaCl. Growth occurred at pH 7.0–11.0 (optimum, pH 9.0–10.0) and 15–40 °C (optimum, 37 °C). Vitamins were not required for growth. The major polar lipids of strain xg18T were phosphatidyl ethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. The major fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The G+C content of genomic DNA was 46.1 mol%. Analysis of 16S rRNA sequences showed that strain xg18T belongs to the genus Nitrincola , with Nitrincola alkalisediminis MEB087T (KC822363, 98.6 %) as its closest neighbour. The DNA–DNA relatedness value of strain xg18T with its closest phylogenetic neighbour, N. alkalisediminis JCM 19317T, was 43.1±3.2 %. Strain xg18T was clearly distinguishable from the type strain of the genus Nitrincola through phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics comparisons. Based on its phenotypic and chemotaxonomic characteristics, strain xg18T could be classified as a representative of a novel species of the genus for which the name Nitrincola tibetensis sp. nov. is proposed. The type strain is xg18T (=CICC 24457T=KCTC 62401T).", }