@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003122, author = "Fang, Bao-Zhu and Han, Ming-Xian and Zhang, Ling-Yu and Jiao, Jian-Yu and Zhang, Xiao-Tong and Zhang, Zi-Tong and Wang, Yan and Nie, Guo-Xing and Li, Wen-Jun", title = "Nocardia aurea sp. nov., a novel actinobacterium isolated from a karstic subterranean environment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "1", pages = "159-164", doi = "https://doi.org/10.1099/ijsem.0.003122", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003122", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "polyphasic taxonomy", keywords = "Nocardia aurea sp. nov.", keywords = "karst cave", abstract = "A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia . DNA–DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars were arabinose, ribose and galactose. The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid. Mycolic acids were present. The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia , for which the name Nocardia aurea sp. nov. is proposed. The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).", }