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Volume 69, Issue 11

sp. nov., a bacterium isolated from an arable soil sample of L. Free

Abstract

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain L4-6, was isolated from an arable soil sample of tobacco in Huize, south-western China and subjected to polyphasic taxonomic characterization. The cells showed oxidase-positive and catalase-positive reactions. Growth occurred at 20–35 °C, at pH 5.0–8.0 and with 0–2 % (w/v) NaCl, optimally at 30 °C, pH 6.0–7.0 and 0–1 % (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-8. The predominant cellular fatty acids (>10.0 %) were identified as summed feature 9 (iso-Cω9 or C 10-methyl), iso-C and iso-C. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified aminophospholipids and five unidentified aminolipids. The genomic DNA G+C content was 60.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain L4-6 should be affiliated to the genus and formed a clade with the most closely related organism JS12-10. 16S rRNA gene sequences similarity analysis showed that strain L4-6 was mostly closely related to JS12-10 (98.73 %) and DHOB07 (98.02 %). DNA–DNA hybridization data indicated that strain L4-6 represented a novel genomic species belonging to the genus . The polyphasic taxonomic characteristics indicated that the strain L4-6 represents a novel species of the genus , for which the name sp. nov. (type strain L4-6=CGMCC 1.16273=KCTC 62035) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Dyella tabacisoli sp. nov. , Nicotiana tabacum L and polyphasic taxonomy
© 2019 IUMS
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2019-11-01
2024-04-24
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Dyella tabacisoli sp. nov., a bacterium isolated from an arable soil sample of Nicotiana tabacum L.
Int J Syst Evol Microbiol 69, 3338 (2019); https://doi.org/10.1099/ijsem.0.003138
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