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Volume 69, Issue 2

Alcanivorax profundi sp. nov., isolated from deep seawater of the Mariana Trench Free

Abstract

A Gram-stain-negative, rod-shaped, non-motile, strictly aerobic strain, designated as MTEO17, was isolated from a 1000 m deep seawater sample of the Mariana Trench. Growth was observed at 10–45 °C (optimum, 37 °C), in the presence of 0.0–12.0 % NaCl (w/v; optimum, 3.0 %) and at pH 6.0–10.0 (optimum, pH 7.0–8.0). Phylogenetic analysis, based on the 16S rRNA gene sequence, revealed that strain MTEO17 belonged to the genus Alcanivorax and showed the highest sequence similarity of 97.9 % to Alcanivorax nanhaiticus MCCC 1A05629. The estimated average nucleotide identity and DNA–DNA hybridization values between strain MTEO17 and A. nanhaiticus MCCC 1A05629 were 78.98 and 23.80 %, respectively. The significant dominant fatty acids were C16 : 0, summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The polar lipids comprised two phosphatidylethanolamines, one phosphatidylglycerol, one unidentified phospholipid and four unidentified polar lipids. The DNA G+C content of strain MTEO17 was 57.5 %. On the basis of the polyphasic evidence, strain MTEO17 is proposed to represent a novel species of the genus Alcanivorax , for which the name Alcanivorax profundi sp. nov. is proposed. The type strain is MTEO17 (=KCTC 52694=MCCC 1K03252).

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Keyword(s): Alcanivorax profundi , Mariana Trench and seawater
© 2019 IUMS
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2018-12-13
2024-04-25
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Alcanivorax profundi sp. nov., isolated from deep seawater of the Mariana Trench
Int J Syst Evol Microbiol 69, 371 (2019); https://doi.org/10.1099/ijsem.0.003145
/content/journal/ijsem/10.1099/ijsem.0.003145
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