@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003157, author = "Wu, Shijuan and Xia, Xian and Zhou, Zijie and Wang, Dan and Wang, Gejiao", title = "Nocardioides gansuensis sp. nov., isolated from geopark soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "2", pages = "390-396", doi = "https://doi.org/10.1099/ijsem.0.003157", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003157", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Nocardioidaceae", keywords = "geopark soil", keywords = "Nocardioides gansuensis", abstract = "A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China. The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01T (97.10 %), Nocardioides aquiterrae GW-9T (97.08 %) and Nocardioides terrigena DS-17T (96.94 %). Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees. The DNA–DNA relatedness of WSJ-1T/ N. sediminis JCM19559T and WSJ-1T/ N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively. Average nucleotide identity values of whole genome sequences of WSJ-1T/ N. terrigena KCTC19217T and WSJ-1T/ N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively. Its genome size was 4.76 Mb, comprising 4517 predicted genes with a DNA G+C content of 70.9 %. Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol. The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides , for which the name Nocardioides gansuensis sp. nov. is proposed. The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T).", }