RT Journal Article SR Electronic(1) A1 Wu, Shijuan A1 Xia, Xian A1 Zhou, Zijie A1 Wang, Dan A1 Wang, GejiaoYR 2019 T1 Nocardioides gansuensis sp. nov., isolated from geopark soil JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 2 SP 390 OP 396 DO https://doi.org/10.1099/ijsem.0.003157 PB Microbiology Society, SN 1466-5034, AB A novel bacterial strain, designated WSJ-1T, was isolated from geopark soil in Gansu province, PR China. The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01T (97.10 %), Nocardioides aquiterrae GW-9T (97.08 %) and Nocardioides terrigena DS-17T (96.94 %). Strain WSJ-1T grouped with N. terrigena DS-17T and N. sediminis MSL-01T in 16S rRNA gene-based phylogenetic trees. The DNA–DNA relatedness of WSJ-1T/ N. sediminis JCM19559T and WSJ-1T/ N. aquiterrae JCM11813T were 44.8 and 29.2 %, respectively. Average nucleotide identity values of whole genome sequences of WSJ-1T/ N. terrigena KCTC19217T and WSJ-1T/ N. sediminis KCTC19271T were 78.83 and 78.83 %, respectively. Its genome size was 4.76 Mb, comprising 4517 predicted genes with a DNA G+C content of 70.9 %. Strain WSJ-1T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol. The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides , for which the name Nocardioides gansuensis sp. nov. is proposed. The type strain is WSJ-1T (=KCTC 49117T=CCTCC AB 2018027T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003157