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Volume 70, Issue 3

sp. nov., isolated from peat swamp forest soil Free

Abstract

A novel actinobacterial strain, designated K81G1, was isolated from a soil sample collected in Kantulee peat swamp forest, Surat Thani Province, Thailand, and its taxonomic position was determined using a polyphasic approach. Optimal growth of strain K81G1 occurred at 28–30 °C, at pH 5.0–6.0 and without NaCl. Strain K81G1 had cell-wall chemotype IV (-diaminopimelic acid as the diagnostic diamino acid, and arabinose and galactose as diagnostic sugars) and phospholipid pattern type II, characteristic of the genus . It contained MK-9(H) as the predominant menaquinone, iso-C, C cyclo and C as the major cellular fatty acids, and phospholipids consisting of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol and two unidentified phospholipids. Based on 16S rRNA gene sequence similarity and phylogenetic analyses, strain K81G1 was most closely related to TBRC 6029 (97.8 % similarity), JCM 30562 (97.8 %) and DSM 45807 (97.6 %). Strain K81G1 exhibited low average nucleotide identity and digital DNA–DNA hybridization values with TBRC 6029 (76.4 %, 23.0 %), JCM 30562 (77.9 %, 24.6 %) and DSM 45807 (77.8 %, 24.3 %). The DNA G+C content of strain K81G1 was 69.7 mol%. Based on data from this polyphasic study, strain K81G1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K81G1 (=TBRC 10047=NBRC 113896).

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  • Published Online:
Keyword(s): actinomycete , Amycolatopsis , peat swamp forest and polyphasic taxonomy
© 2020 The Authors
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2019-12-18
2024-05-10
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Amycolatopsis acidicola sp. nov., isolated from peat swamp forest soil
Int J Syst Evol Microbiol 70, 1547 (2020); https://doi.org/10.1099/ijsem.0.003933
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