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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 3

sp. nov. and sp. nov., two new species of the genus , isolated from deep-sea sediment samples No Access

Abstract

Two Gram-positive, non-motile, short rod-shaped actinomycete strains, designated as A18JL241 and Y20, were isolated from deep-sea sediment samples collected from the Southwest Indian Ocean and Western Pacific Ocean, respectively. Both of the isolates were able to grow within the temperature range of 5–40 °C, NaCl concentration range of 0–7  % (w/v) and at pH 6.0–12.0. The two most abundant cellular fatty acids of both strains were anteiso-C and anteiso-C. The major polar lipid contents of the two strains were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one unidentified glycolipid. These two strains shared common chemotaxonomic features comprising MK-10 and MK-12 as the respiratory quinones. The genomic DNA G+C contents of the two strains were 68.1 and 70.4  mol%, respectively. The 16S rRNA gene phylogeny showed that the novel strains formed two distinct sublines within the genus . Strain A18JL241 was most closely related to the type strain of KCTC 49593 (98.8 % sequence similarity), whereas strain Y20 formed a tight cluster with the type strain of NBRC 15075 (99.0 %). The orthologous average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values with the type strains of related species were in the range of 74.1–89.1  % and 19.4–36.9  %, respectively, which were below the recognized thresholds of 95–96 % ANI and 70 % dDDH for species definition. Based on the results obtained here, it can be concluded that strains A18JL241 and Y20 represent two novel species of the genus , for which the names sp. nov. (type strain A18JL241=JCM 33956=MCCC 1A16622) and sp. nov. (type strain Y20=JCM 33960=MCCC 1A16747) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): actinomycetes , Actinomycetota , Microbacterium , novel species , polyphasic taxonomy and sediment
Funding
This study was supported by the:
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (Award NO. 2019011)
    • Principle Award Recipient: GaiyunZhang
© 2024 The Authors
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2024-03-25
2024-04-27
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Microbacterium abyssi sp. nov. and Microbacterium limosum sp. nov., two new species of the genus Microbacterium, isolated from deep-sea sediment samples
Int J Syst Evol Microbiol 74, 006299 (2024); https://doi.org/10.1099/ijsem.0.006299
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