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Volume 74, Issue 3

sp. nov., isolated from the rhizosphere of multiple cash crops in China Open Access

Abstract

Three bacterial strains, FP250, FP821, and FP53, were isolated from the rhizosphere soil of oilseed rape, licorice, and habanero pepper in Anhui Province, Xinjiang Uygur Autonomous Region, and Jiangsu Province, PR China, respectively. All strains were shown to grow at 4–37 °C and pH 6.0–9.0, and in the presence of 0–4.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences or housekeeping genes (16S rRNA, , , and ) and phylogenomic analysis showed that strains FP250, FP821, and FP53 belong to the genus , and are closely related to DSM 13647, JCM 11938, 11K1, and DSM 13194. The DNA G+C content of strain FP205 was 59.8 mol%. The average nucleotide identity and digital DNA–DNA hybridization values of strain FP205 with the most closely related strain were 93.2 % and 51.4 %, respectively, which is well below the threshold for species differentiation. Strain FP205 contained summed feature 3 (C 6 and/or C 7), summed feature 8 (C 7 and/or C 6) as major fatty acids, and diphosphatidylglycerol along with phosphatidylethanolamine and aminophospholipid as major polar lipids. The predominant isoprenoid quinone was ubiquinone-9. Based on these phenotypic, phylogenetic, and chemotaxonomic results, strain FP205 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FP205 (=ACCC 62447=JCM 35687).

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Keyword(s): crops rhizosphere , plant growth-promoting rhizobacteria , polyphasic taxonomy and Pseudomonas
Funding
This study was supported by the:
  • the National Key R&D Program of China (Award 2022YFD1901300)
    • Principle Award Recipient: WeiHai-Lei
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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Pseudomonas hefeiensis sp. nov., isolated from the rhizosphere of multiple cash crops in China
Int J Syst Evol Microbiol 74, 006303 (2024); https://doi.org/10.1099/ijsem.0.006303
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