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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 3

sp. nov., isolated from sediment of tidal flat located in Zhoushan, China No Access

Abstract

A Gram-stain-negative, aerobic, motile with flagella and rod- or ovoid-shaped bacterium, designated GG15, was isolated from tidal flat sediment sampled in Zhoushan, Zhejiang Province. Strain GG15 grew at 20–40 °C (optimum, 30 °C), at pH 5.5–9.5 (optimum, pH 7.0–8.0) and with 1.0–10.0 % (w/v) NaCl (optimum, 1.5 %). Colony diameters ranged from 1 to 3 mm within the first week, reaching a maximum of 6–7 mm after 15 days of cultivation. Strain GG15 exhibited highest 16S rRNA gene sequence similarity to CCM 7856 (98.1 %), with similarity to other species within the genus ranging from 97.8 to 93.8 %. Similarity values to other genera were below 93.8 %. Strain GG15 exhibited positive activity for -glucosidase, trypsin and chymotrypsin, whereas the reference strain showed negative activity. Chemotaxonomic analyses indicated that strain GG15 contained Q-8 as the sole respiratory quinone, C (9.1 %), iso-C (30.9 %) and iso-C 3-OH (7.2 %) as the predominant fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, four unidentified glycolipids, one unidentified phospholipid, two unidentified aminolipids and two unidentified aminophospholipids as the main polar lipids. The genome of strain GG15 was 4 307 641 bp long, comprising 3861 protein-coding genes. The G+C content of strain GG15 was 61.5 mol% based on its genomic sequence. Strain GG15 showed low digital DNA–DNA hybridization (<70 %) and average nucleotide identity values (<95 %) with other species. As a result, a novel species within the genus , named sp. nov., is proposed. The type strain is GG15 (MCCC 1K08802=KCTC 8210).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Microbulbifer magnicolonia , Microbulbiferaceae , tidal flat and Zhoushan
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31900003)
    • Principle Award Recipient: CongSun
  • National Natural Science Foundation of China (Award 82002208)
    • Principle Award Recipient: Xiao-YunYu
  • Key R&D Program of Zhejiang (Award #2023C03011)
    • Principle Award Recipient: CongSun
  • Zhejiang Provincial Natural Science Foundation of China (Award LDT23D06025D06)
    • Principle Award Recipient: Xiao-MingXia
© 2024 The Authors
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2024-04-27
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Microbulbifer magnicolonia sp. nov., isolated from sediment of tidal flat located in Zhoushan, China
Int J Syst Evol Microbiol 74, 006310 (2024); https://doi.org/10.1099/ijsem.0.006310
/content/journal/ijsem/10.1099/ijsem.0.006310
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