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Volume 74, Issue 4

sp. nov., sp. nov. and sp. nov., rare actinobacteria from Miocene lacustrine sediment of the Sokolov Coal Basin, Czech Republic Open Access

Abstract

The taxonomic position of three actinobacterial strains, BCCO 10_0061, BCCO 10_0798, and BCCO 10_0856, recovered from bare soil in the Sokolov Coal Basin, Czech Republic, was established using a polyphasic approach. The multilocus sequence analysis based on 100 single-copy genes positioned BCCO 10_0061 in the same cluster as , strain BCCO 10_0798 in the same cluster as , , , and , and strain BCCO 10_0856 clustered together with and . Morphological and chemotaxonomic characteristics of these strains support their assignment to the genus . In all three strains, MK-9(H) accounted for more than 80 % of the isoprenoid quinone. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The whole-cell sugars were rhamnose, ribose, mannose, glucose, and galactose. The major fatty acids (>10 %) were iso-C, anteiso-C, iso-C, and C. The polar lipids were diphosphatidylglycerol, methyl-phosphatidylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol. The genomic DNA G+C content of strains (mol%) was 68.8 for BCCO 10_0061, 69.2 for BCCO 10_0798, and 68.5 for BCCO 10_0856. The combination of digital DNA–DNA hybridization results, average nucleotide identity values and phenotypic characteristics of BCCO 10_0061, BCCO 10_0798, and BCCO 10_0856 distinguishes them from their closely related strains. Bioinformatic analysis of the genome sequences of the strains revealed several biosynthetic gene clusters (BGCs) with identities >50 % to already known clusters, including BGCs for geosmin, coelichelin, ε-poly--lysine, and erythromycin-like BGCs. Most of the identified BGCs showed low similarity to known BGCs (<50 %) suggesting their genetic potential for the biosynthesis of novel secondary metabolites. Based on the above results, each strain represents a novel species of the genus , for which we propose the name sp. nov. for BCCO 10_0061 (=DSM 116175), sp. nov. for BCCO 10_0798 (=DSM 116176), and sp. nov. for BCCO 10_0856 (=DSM 116177).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomycetes , Lentzea , polyphasic taxonomy and post-mining sites
Funding
This study was supported by the:
  • Leibniz Association through the Collaborative Excellence (Award K445/2022)
    • Principle Award Recipient: YvonneMast
  • Deutsches Zentrum für Infektionsforschung (Award TTU 09.819)
    • Principle Award Recipient: YvonneMast
  • Ministerstvo školství, mládeže a tělovýchovy České republiky (Award OP VVV CZ.02.2.69/0.0/0.0/18_054/0014649, ABERA - Anchoring the Biological Centre AV ČR, v. v. i. in the European Research Area)
    • Principle Award Recipient: ApplicableNot
  • Ministerstvo školství, mládeže a tělovýchovy České republiky (Award OP VVV CZ.02.1.01/0.0/0.0/18_046/0016045 and Czech-BioImaging LM2023050)
    • Principle Award Recipient: ApplicableNot
  • Akademie věd České republiky (Award Strategie AV21)
    • Principle Award Recipient: ChroňákováAlica
  • Ministerstvo Zdravotnictví České Republiky (Award No. 17-30091A)
    • Principle Award Recipient: ChroňákováAlica
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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Lentzea sokolovensis sp. nov., Lentzea kristufekii sp. nov. and Lentzea miocenica sp. nov., rare actinobacteria from Miocene lacustrine sediment of the Sokolov Coal Basin, Czech Republic
Int J Syst Evol Microbiol 74, 006335 (2024); https://doi.org/10.1099/ijsem.0.006335
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