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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 4

sp. nov., isolated from the faeces of rhinoceros and reclassification of as comb. nov. No Access

Abstract

Strain Ran72, a novel Gram-stain-negative, obligately aerobic, non-motile, and rod-shaped bacterium, was isolated from the faeces of the rhinoceros species . The novel bacterial strain grew optimally in Reasoner's 2A medium under the following conditions: 0 % (w/v) NaCl, pH 7.5, and 30 °C. Based on phylogenetic analysis using 16S rRNA gene sequencing, strain Ran72 was found to be most closely related to F4 (98.4 %), DKR-2 (98.0 %), and H38 (97.4 %). A comprehensive genome-level comparison between strain Ran72 with F4, DKR-2, and H38 revealed average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity values of ≤74.9, ≤19.3, and ≤78.7 %, respectively. The major fatty acids were anteiso-C (22.3 %), with MK-6 being the predominant respiratory quinone. The major polar lipids of strain Ran72 were phosphatidylethanolamine, four unidentified aminolipids, and two unidentified lipids. Based on our chemotaxonomic, genotypic, and phenotype characterizations, strain Ran72 was identified as representing a novel species in the genus , for which the name sp. nov. is proposed, with the type strain Ran72 (=KACC 23136=JCM 36038). Based on the outcomes of our phylogenomic study, should be reclassified under the genus as comb. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): animal faeces , Bacteroidota , Kaistella , rhinoceros , taxonomy and Weeksellaceae
Funding
This study was supported by the:
  • Korea University grant (Award K2309811)
    • Principle Award Recipient: WoojunPark
© 2024 The Authors
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2024-04-11
2024-04-30
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Kaistella rhinocerotis sp. nov., isolated from the faeces of rhinoceros and reclassification of Chryseobacterium faecale as Kaistella faecalis comb. nov.
Int J Syst Evol Microbiol 74, 006338 (2024); https://doi.org/10.1099/ijsem.0.006338
/content/journal/ijsem/10.1099/ijsem.0.006338
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