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Volume 74, Issue 4

sp. nov., isolated from fresh water No Access

Abstract

A novel Gram-stain-negative, yellow-pigmented, short rod-shaped bacterial strain, HBC34, was isolated from a freshwater sample collected from Daechung Reservoir, Republic of Korea. The results of 16S rRNA gene sequence analysis indicated that HBC34 was affiliated with the genus and shared the highest sequence similarity to the type strains of (98.01 %), (97.87 %) and (97.59 %). The average nucleotide identity (ANI) and digital DNA–DNA hybridisation (dDDH) values between HBC34 and species of the genus with validly published names were below 84.01 and 28.1 %, respectively. These values were lower than the accepted species-delineation thresholds, supporting its recognition as representing a novel species of the genus . The major fatty acids (>10 % of the total fatty acids) were identified as summed feature 8 (Cω7 and/or Cω6) and summed feature 3 (Cω7 and/or Cω6). The main polar lipids were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, two phospholipids and two unidentified polar lipids. The respiratory quinone was Q-10. The genomic DNA G+C content of HBC34 was 64.04 %. The polyphasic evidence supports the classification of HBC34 as the type strain of a novel species of the genus for which the name sp. nov is proposed. The type strain is HBC34 (= KCTC 8002= LMG 33140).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cyanobacterial bloom , periphyton , Sphingobium and Sphingobium cyanobacteriorum
Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology (Award KGM5252322)
    • Principle Award Recipient: Chi-YongAhn
  • National Research Foundation (Award 2023R1A2C1003308)
    • Principle Award Recipient: Chi-YongAhn
  • Korea Environmental Industry and Technology Institute (Award 2022003050004)
    • Principle Award Recipient: Chi-YongAhn
© 2024 The Authors
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2024-04-17
2024-04-30
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Sphingobium cyanobacteriorum sp. nov., isolated from fresh water
Int J Syst Evol Microbiol 74, 006339 (2024); https://doi.org/10.1099/ijsem.0.006339
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