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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 4

gen. nov., sp. nov., a novel taxon within the family isolated from sediment of a river No Access

Abstract

A Gram-stain-negative bacterium, designated XZ-24, was isolated from sediment of a river in Mianyang city, Sichuan province, PR China. Cells (1.0–2.0 µm long and 0.4–0.5 µm in width) were strictly aerobic, non-spore-forming, rod shaped, prosthecate and motile by means of a polar flagellum. Growth occurred at 10–37 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum pH 7.0) and with 0–3.0 % (w/v) NaCl (optimum 1.0 % NaCl). The results of phylogenetic analysis based on genomes and 16S rRNA gene sequences indicated that XZ-24 formed a distinct phyletic branch within the family and was most closely related to members of the genera , and with 95.3–96.5 % 16S rRNA gene sequence similarities. The average amino acid identities (AAI) between XZ-24 and species of the family were 47.0–64.5 %, which were below the genus boundary (70 %). The predominant cellular fatty acids were summed feature 8 (Cω7 and/or Cω6), C, Cω7 11-methyl and summed feature 3 (Cω7 and/or Cω6), the isoprenoid quinone was Q-10, and the major polar lipids were 1,2-di-O-acyl-3-O-α--glucopyranuronosyl glycerol; 1,2-di-O-acyl-3-O-[-glucopyranosyl-(1→4)-α- glucopyranuronosyl] glycerol and phosphatidylglycerol. The genome size of XZ-24 was 2.64 Mb with a DNA G+C content of 68.9 %. On the basis of the evidence presented in this study, strain XZ-24 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. (Type strain XZ-24=CCTCC AB 20 23 094=KCTC 8038) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , average amino acid identity , Peiella sedimenti gen. nov., sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • National Key Research and Development Program of China (Award 2023YFD1500904-2)
    • Principle Award Recipient: JianHe
  • Special Fund for Science and Technology Program of Jiangsu Province (Award BM2022019)
    • Principle Award Recipient: JianHe
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 32170128)
    • Principle Award Recipient: JianHe
© 2024 The Authors
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Peiella sedimenti gen. nov., sp. nov., a novel taxon within the family Caulobacteraceae isolated from sediment of a river
Int J Syst Evol Microbiol 74, 006344 (2024); https://doi.org/10.1099/ijsem.0.006344
/content/journal/ijsem/10.1099/ijsem.0.006344
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