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Volume 74, Issue 5

sp. nov., a novel black yeast isolated from the gut of amphipods in Thailand No Access

Abstract

The genus is polymorphic, able to transition between yeast, hyphal and pseudohyphal forms. Species of the genus are ubiquitous fungi that are distributed in various environments around the world. During a survey of fungal diversity in the gut of amphipods ( and undescribed Dogielinotid amphipods) from the Amphawa estuary, Samut Songkhram province, Thailand, five black yeast strains (DMKU-MG01, DMKU-MG07, DMKU-MG08, DMKU-HG10 and DMKU-FG04) were identified as representing a novel taxon on the basis of a combination of morphological and molecular phylogenetic features. The five strains did not produce filamentous hyphae or pseudohyphae. Only budding yeast cells were observed. On the basis of the phenotypic characteristics and the results of molecular analyses of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, the five strains were identified as representing a novel species via applied nucleotide pairwise analysis. They differed from the most closely related species by 3.54 % nucleotide substitutions (20 nucleotide substitutions in 572 bp) in the D1/D2 domains of the LSU rRNA gene. Moreover, the sequences of the ITS region of the five strains differed from those of the most closely related species , by 7.44–9.62 % nucleotide substitutions, and , by 7.2–7.53 % nucleotide substitutions. The results of phylogenetic analyses based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene confirmed that the five black yeast strains represented a single novel species of the genus . In this study, sp. nov. is proposed to accommodate these strains. The holotype is TBRC 15626 and the isotype is PYCC9020. The MycoBank accession number of the novel species is MB 851477.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): amphipods , black yeast , digestive tract , Exophiala amphawaensis and novel fungi
Funding
This study was supported by the:
  • Graduate Scholarship from Faculty of Science, Kasetsart University (Award -)
    • Principle Award Recipient: HarantoTita
  • Fundamental Fund Basic research (Kasetsart University (KU) in fiscal year 2566) (Award FF(KU)19.66)
    • Principle Award Recipient: PiyangkunLueangjaroenkit
© 2024 The Authors
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2024-05-19
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Exophiala amphawaensis sp. nov., a novel black yeast isolated from the gut of amphipods in Thailand
Int J Syst Evol Microbiol 74, 006363 (2024); https://doi.org/10.1099/ijsem.0.006363
/content/journal/ijsem/10.1099/ijsem.0.006363
/content/journal/ijsem/10.1099/ijsem.0.006363
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