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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 5

sp. nov., a plant-beneficial bacterium isolated from litchi rhizosphere soil in Guangdong, China No Access

Abstract

A novel plant-beneficial bacterium strain, designated as JGH33, which inhibited sporangia germination, was isolated on Reasoner's 2A medium from a litchi rhizosphere soil sample collected in Gaozhou City, Guangdong Province, PR China. Cells of strain JGH33 were Gram-stain-positive, aerobic, non-motile, bent rods. The strain grew optimally at 30–37 °C and pH 6.0–8.0. Sequence similarity analysis based on 16S rRNA genes indicated that strain JGH33 exhibited highest sequence similarity to LC13 (99.2 %). The genomic DNA G+C content of the isolate was 69.1 mol%. The genome of JGH33 was 4.7 Mbp in size with the average nucleotide identity value of 83.45 % to the most related reference strains, which is lower than the species delineation threshold of 95 %. The digital DNA–DNA hybridization of the isolate resulted in a relatedness value of 24.9 % with its closest neighbour. The predominant respiratory quinone of JGH33 was MK-9(H). The major fatty acids were C anteiso (43.4 %), C iso (19.1 %) and C anteiso (19.3 %), and the featured component was C ω6 (1.01 %). The polar lipid composition of strain JGH33 included diphosphatidylglycerol, phosphatidylglycerol, dimannosylglyceride, phosphatidylinositol and glycolipids. On the basis of polyphasic taxonomy analyses data, strain JGH33 represents a novel species of the genus , for which the name sp. nov. is proposed, with JGH33 (=JCM 35868=GDMCC 1.3730) as the type strain.

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Keyword(s): litchi rhizosphere soil , plant-beneficial bacterium , polyphasic taxonomy , Sinomonas and strain JGH33T
Funding
This study was supported by the:
  • Guangdong Strategic Special Fund for Rural Revitalization (Award 2023-WBH-00-001)
    • Principle Award Recipient: HonghuiZhu
  • Guangdong Special Support Plan (Award 2021JC06N628)
    • Principle Award Recipient: HonghuiZhu
  • the open competition program of top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province (Award 2022SDZG09 and 2023SDZG09)
    • Principle Award Recipient: HonghuiZhu
  • Basic and Applied Basic Research Foundation of Guangdong Province (Award 2022A1515010314)
    • Principle Award Recipient: YangZhou
  • National Natural Science Foundation of China (Award 32370122)
    • Principle Award Recipient: YangZhou
© 2024 The Authors
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Sinomonas terricola sp. nov., a plant-beneficial bacterium isolated from litchi rhizosphere soil in Guangdong, China
Int J Syst Evol Microbiol 74, 006375 (2024); https://doi.org/10.1099/ijsem.0.006375
/content/journal/ijsem/10.1099/ijsem.0.006375
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