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Volume 74, Issue 5

sp. nov. and sp. nov., isolated from marine brown algae Open Access

Abstract

Two Gram-stain-negative, rod-shaped bacteria, designated as strains KJ10-1 and KJ40-1, were isolated from marine brown algae. Both strains were catalase-positive, oxidase-positive, and facultative aerobic. Strain KJ10-1 exhibited optimal growth at 25 °C, pH 7.0, and 3 % NaCl, whereas strain KJ40-1 showed optimal growth at 25 °C, pH 7.0, and 2 % NaCl. The respiratory quinones of strain KJ10-1 were ubiquinone-8, ubiquinone-7, menaquinone-7, and methylated menaquinone-7, while the respiratory quinone of strain KJ40-1 was only ubiquinone-8. As major fatty acids, strain KJ10-1 contained C, C ω8, iso-C, and summed feature 3 (C 7 and/or C 6) and strain KJ40-1 contained C and summed features 3 and 8 (C 7 and/or C 6). The major polar lipids in strain KJ10-1 were phosphatidylethanolamine, phosphatidylglycerol, and an unidentified aminolipid, whereas those in strain KJ40-1 were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C contents of strains KJ10-1 and KJ40-1 were 42.1 and 40.8 mol%, respectively. Based on 16S rRNA gene sequences, strains KJ10-1 and KJ40-1 exhibited the closest relatedness to MMS16-UL250 (98.6 %) and S-1 (95.4 %), respectively. Phylogenetic analyses, based on both 16S rRNA and 92 housekeeping genes, showed that the strains formed distinct phylogenic lineages within the genera and . Digital DNA–DNA hybridization and orthologous average nucleotide identity values between strain KJ10-1 and other species, as well as between strain KJ40-1 and other species, were below the thresholds commonly accepted for prokaryotic species delineation. Based on the phenotypic, chemotaxonomic, and phylogenetic data, strains KJ10-1 and KJ40-1 represent novel species of the genera and , respectively, for which the names sp. nov. and sp. nov. are proposed, respectively. The type strains of and are KJ10-1 (=KACC 22589=JCM 35409) and KJ40-1 (=KACC 22588=JCM 35410), respectively.

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Keyword(s): brown algae , new taxa , Pseudomonadota , Shewanella phaeophyticola and Vibrio algarum
Funding
This study was supported by the:
  • National Institute of Biological Resources (KR)
    • Principle Award Recipient: CheOk Jeon
  • Chung-Ang University
    • Principle Award Recipient: JaejoonJung
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-05-10
2024-05-20
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Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov., isolated from marine brown algae
Int J Syst Evol Microbiol 74, 006378 (2024); https://doi.org/10.1099/ijsem.0.006378
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