- Volume 52, Issue 6, 2002
Volume 52, Issue 6, 2002
- Articles
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Characterization of a novel halophilic archaeon, Halobiforma haloterrestris gen. nov., sp. nov., and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov.
More LessStrain 135(T), a novel red-pigmented, aerobic, extremely halophilic member of the Archaea showing rod, coccus and slightly pleomorphic morphology, was isolated from hypersaline soil close to Aswan (Egypt). This organism is neutrophilic, motile and requires at least 2.2 M NaCl, but no MgCl2, for growth and exhibits optimal growth at 42 degrees C. Polar lipid analysis revealed the presence of sulfated triglycosyl diether and triglycosyl diether as the sole glycolipids as well as the absence of the glycerol diether analogue of phosphatidyl glycerosulfate. C20:C20 and C20:C25 core lipids are present in almost equal proportions. The G+C content of the DNA is 66.9 mol%. 16S rDNA analysis revealed that strain 135(T) was a member of the phyletic group defined by the family Halobacteriaceae, but there was a low degree of similarity to other members of this family. Highest similarity values of 96.4 and 93.8-94.3% were obtained to the 16S rDNA of Natronobacterium nitratireducens and Natronobacterium gregoryi, Natronococcus occultus and Natronococcus amylolyticus. Strain 135(T) is able to accumulate polyhydroxybutyrate as intracellular reserve material. On the basis of the data presented, strain 135(T) should be placed in a new genus, Halobiforma gen. nov. as Halobiforma haloterrestris sp. nov. The type strain is strain 135(T) (= DSM 13078(T) = JCM 11627(T)). Moreover, the transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. is proposed.
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Characterization of Serratia isolates from soil, ecological implications and transfer of Serratia proteamaculans subsp. quinovora Grimont et al. 1983 to Serratia quinivorans corrig., sp. nov.
More LessEleven strains of Serratia were isolated from different soils and the guts of invertebrates and characterized by their sensitivity to eight indigenous bacteriophages. They were also classified according to bacteriocin production and sensitivity, BiOLOG plate and API 20E strip profiles and 16S rRNA sequence information. One strain was thus identified as Serratia plymuthica, another as Serratia fonticola. The remaining strains were shown to be closely related to Serratia proteamaculans subsp. quinovora Grimont et al. 1983 after DNA-DNA cross-hybridization demonstrated relatedness greater than 70% with the type strain of this subspecies. From an ecological perspective, our results illustrated the wide variation in sensitivity that closely related Serratia strains have towards various indigenous soil phages and that these phages have broad host ranges within the genus. Furthermore, the phage and bacteriocin interactions within the Serratia strains examined were intricate and did not reflect phylogenetic relationships. These results together imply that complex interactions will occur in soil within the natural community of Serratia strains and their bacteriophages. DNA-DNA cross-hybridization and phenotypic characterization showed that S. proteamaculans subsp. quinovora strains formed a cohesive group at the species level. It is therefore concluded that these strains should be designated as Serratia quinivorans corrig., sp. nov.
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Nocardiopsis metallicus sp. nov., a metal-leaching actinomycete isolated from an alkaline slag dump.
A taxonomic study was carried out on a metal-mobilizing, alkaliphilic bacterium from an alkaline slag dump, strain KBS6(T). The strain produced substrate and aerial mycelia. Growth occurred in the pH range 7.0-10.5, with an optimum at pH 8.5. A salt concentration of up to 10% was tolerated, and various organic substrates were used for growth. The results of a 16S rDNA sequence comparison revealed that strain KBS6(T) belongs to the genus Nocardiopsis. DNA-DNA hybridization with the two closest relatives, Nocardiopsis exhalans and Nocardiopsis prasina, gave similarity values of 18.2 and 44.1%, respectively, which indicated that strain KBS6(T) represents a novel species of the genus Nocardiopsis. This is consistent with the morphological, physiological and chemotaxonomic data. Because of the ability of this micro-organism to solubilize metals, the name Nocardiopsis metallicus sp. nov. is proposed for strain KBS6(T) (= DSM 44598(T) = NRRL B-24159(T)), this being the type strain.
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Phylogenetic relationships amongst tetrahymenine ciliates inferred by a comparison of telomerase RNAs.
More LessThe phylogenetic relationships between ciliate species in the suborder Tetrahymenina, order Hymenostomata, was investigated by comparing their telomerase RNA (TER) sequences. This relatively small RNA is an integral part of telomerase, the ribonucleoprotein enzyme that catalyses the synthesis of telomeric DNA. Despite a relatively rapid rate of primary sequence divergence, conserved functional and structural elements within TERs facilitate the accurate alignment of truly homologous nucleotides. The tetrahymenine phylogeny derived from distance analysis of TER sequences is largely consistent with those based on rRNA and histone sequences.
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Cryptococcus statzelliae sp. nov. and three novel strains of Cryptococcus victoriae, yeasts isolated from Antarctic soils.
More LessA morphological and physiological characterization of yeast strains CBS 8908, CBS 8915, CBS 8920, CBS 8925(T) and CBS 8926, isolated from Antarctic soils, was performed. Phylogenetic analyses of the sequences of the D1/D2 regions and the adjacent internal transcribed spacer (ITS) regions of the large-subunit rDNA of these strains placed them into the Tremellales clade of the Hymenomycetes. The sequence data identified strains CBS 8908, CBS 8915 and CBS 8920 as belonging to the species Cryptococcus victoriae. Strains CBS 8925(T) and CBS 8926 were found to represent an unique clade within the Hymenomycetes, with Dioszegia crocea CBS 6714(T) being their closest phylogenetic relative. Fatty acid composition and proteome fingerprint data for these novel strains were also obtained. No sexual state was observed. A novel basidiomycetous species, Cryptococcus statzelliae, is proposed for strains CBS 8925(T) and CBS 8926.
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Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov.
More LessMore than 50 ballistoconidium-forming yeast strains, isolated from plant leaves collected in Yunnan, China, were identified as Sporobolomyces roseus Kluyver & van Niel by conventional methods. However, comparison of the internal transcribed spacer (ITS) region and 265 rDNA D1/D2 domain sequences indicated that these strains represented more than one species. Type or authentic strains of the synonyms of Sporobolomyces roseus and the closely related species Sporidiobolus pararoseus Fell & Tallman were employed in the rDNA sequence comparison. Sporobolomyces boleticola Ramírez, Sporobolomyces pollaccii Verona & Ciferri, Sporobolomyces roseus var. madurae Janke and Torulopsis somala Verona were confirmed to be conspecific with Sporobolomyces roseus. Another synonym of this species, Sporobolomyces salmoneus Derx, was located together with Sporobolomyces marcillae Santa Maria in a separate clade. Two synonyms of Sporidiobolus pararoseus, Sporobolomyces carnicolor Yamasaki & Fujii (nom. inval.) and Sporobolomyces japonicus Iizuka & Goto, were revealed to represent two distinct species. The name Sporobolomyces carnicolor is validated, with strain CBS 4215(T) as the type strain. A novel species represented by five of the selected Yunnan strains was confirmed, for which the name Sporobolomyces phaffii sp. nov. is proposed (type strain CH 2.052(T) = AS 2.2137(T) = JCM 11491(T) = CBS 9129(T)). This study also indicates that yeast species with similar ITS sequences may have quite different D1/D2 sequences.
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Citeromyces siamensis sp. nov., a novel halotolerant yeast isolated in Thailand.
More LessTwo halotolerant yeast strains, H130(T) and H149, were isolated from dry salted squid and fermented soybeans, respectively, in Thailand. Both isolates grew by multilateral budding, produced asci that had one roughened spherical ascospore and contained ubiquinone Q-8. These characteristics were shared by Citeromyces matritensis, the only species of the genus Citeromyces. Strains H130(T) and H149 were differentiated from C matritensis by their ability to assimilate L-sorbose and L-lysine and to grow at 37 degrees C. The novel isolates were more tolerant to higher concentrations of cations (3 M NaCl or 0.8 M LiCI) and to higher osmotic pressure (60% glucose) than C. matritensis. A phylogenetic analysis of 18S rRNA gene sequence data indicated that the two novel isolates represented a sister species to C. matritensis. Furthermore, DNA-DNA hybridization data indicated that the isolates were clearly distinct from the type strain of C. matritensis (IFO 0954(T). Based on the above characteristics, strains H130(T) and H149 are proposed to represent a novel species within the genus Citeromyces, Citeromyces siamensis; the type strain is H130(T) (= IFO 11052(T) = JCM 11522(T) = TISTR 5777(T) = CBS 9153(T)).
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Necessary corrections to the Approved Lists of Bacterial Names according to Rule 40d (formerly Rule 46). Request for an opinion.
More LessAccording to Rule 40d (formerly Rule 46), the authorship and dates of ten subspecies cited in the Approved Lists of Bacterial Names should be corrected. It is requested that such changes be approved by the Judicial Commission.
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Volumes and issues
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Volume 74 (2024)
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Volume 73 (2023)
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Volume 72 (2022 - 2023)
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Volume 71 (2020 - 2021)
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Volume 70 (2020)
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Volume 69 (2019)
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Volume 68 (2018)
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Volume 67 (2017)
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Volume 66 (2016)
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Volume 65 (2015)
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Volume 64 (2014)
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Volume 63 (2013)
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Volume 62 (2012)
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Volume 53 (2003)
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Volume 50 (2000)
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Volume 48 (1998)
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Volume 47 (1997)
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Volume 46 (1996)
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Volume 45 (1995)
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Volume 43 (1993)
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Volume 41 (1991)
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Volume 36 (1986)
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Volume 35 (1985)
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Volume 34 (1984)
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Volume 33 (1983)
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Volume 32 (1982)
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Volume 31 (1981)
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Volume 30 (1980)
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Volume 29 (1979)
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Volume 28 (1978)
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Volume 27 (1977)
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Volume 26 (1976)
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Volume 25 (1975)
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Volume 24 (1974)
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Volume 23 (1973)
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Volume 22 (1972)
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Volume 21 (1971)
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Volume 19 (1969)
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Volume 16 (1966)
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Volume 14 (1964)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)