- Volume 58, Issue 10, 2008
Volume 58, Issue 10, 2008
- Notification List
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Notification that new names and new combinations have appeared in volume 58, part 7, of the IJSEM
This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed according to the Rules of priority (i.e. page number and order of valid publication of names in the original articles). Taxonomic opinions included in this List (i.e. the creation of synonyms or the emendation of circumscriptions) cannot be considered as validly published nor, in any other way, approved by the International Committee on Systematics of Prokaryotes and its Judicial Commission.
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- New Taxa
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- Actinobacteria
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Nocardioides koreensis sp. nov., Nocardioides bigeumensis sp. nov. and Nocardioides agariphilus sp. nov., isolated from soil from Bigeum Island, Korea
More LessThree Gram-positive, coccoid to rod-shaped actinobacteria, designated strains MSL-09T, MSL-19T and MSL-28T, were isolated from a soil sample collected from Bigeum Island in Korea, and were subjected to a polyphasic taxonomic analysis. All three isolates grew optimally at pH 7.5–9.0 and 28–30 °C. 16S rRNA gene analysis revealed that all three strains belonged to the genus Nocardioides, with strains MSL-09T, MSL-19T and MSL-28T respectively showing the highest sequence similarity to Nocardioides aquaticus DSM 11439T (96.25 %), Nocardioides aquiterrae GW-9T (95.75 %) and Nocardioides terrigena DS-17T (95.61 %). Chemotaxonomically, they contain ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and diphosphatidylglycerol, phosphatidylglycerol and some unknown lipids as the polar lipids found in the cell wall. iso-C16 : 0 is a major fatty acid. The G+C content of the DNA was respectively 69.9, 69.3 and 69.4 mol% for strains MSL-09T, MSL-19T and MSL-28T. Based on morphological, physiological, biochemical and chemotaxonomic characters presented in this study, the three strains represent novel species of the genus Nocardioides. The names Nocardioides koreensis sp. nov. (type strain MSL-09T =KCTC 19272T =DSM 19266T), Nocardioides bigeumensis sp. nov. (type strain MSL-19T =KCTC 19290T =DSM 19320T) and Nocardioides agariphilus sp. nov. (type strain MSL-28T =KCTC 19276T =DSM 19323T) are proposed.
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Agrococcus jejuensis sp. nov., isolated from dried seaweed
More LessA novel marine actinomycete, designated strain SSW1-48T, was isolated from a dried seaweed sample collected from a beach in Jeju, Republic of Korea. Cells of the organism were non-motile, non-endospore-forming, Gram-positive and rod-shaped. Colonies were circular, smooth, translucent and yellow-coloured. The diagnostic diamino acid in the peptidoglycan was 2,4-diaminobutyric acid and the murein was of the acetyl type. Mycolic acids were absent. The predominant menaquinones were MK-10 and MK-9. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. The G+C content of the DNA was 73.0 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct branch between Agrococcus lahaulensis and Agrococcus casei in the family Microbacteriaceae. The values for 16S rRNA gene sequence similarity between strain SSW1-48T and type strains of recognized Agrococcus species were in the range 95.5–97.5 % and those between this bacterium and other representatives of the family Microbacteriaceae were in the range 93.7–96.4 %. On the basis of the combined data from the phenotypic and phylogenetic analyses, strain SSW1-48T represents a novel species of the genus Agrococcus, for which the name Agrococcus jejuensis sp. nov. is proposed. The type strain is SSW1-48T (=KCTC 19198T=JCM 14256T).
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Reclassification of Streptomyces flavidofuscus as a synonym of Nocardiopsis dassonvillei subsp. dassonvillei
More LessDuring the course of quality control studies of the collection of the NITE Biological Resource Center (NBRC), phylogenetic analysis based on 16S rRNA gene sequences of actinomycetes revealed that Streptomyces flavidofuscus NBRC 15404T formed a cluster with Nocardiopsis dassonvillei and Nocardiopsis synnemataformans. Strain NBRC 15404T contained meso-diaminopimelic acid as a cell-wall diamino acid and DNA–DNA hybridization studies also showed that S. flavidofuscus NBRC 15404T was a close relative of N. dassonvillei subsp. dassonvillei NBRC 14626T. Based on chemotaxonomic, phenotypic and genetic analysis of the type strain, Streptomyces flavidofuscus should be reclassified as a later heterotypic synonym of Nocardiopsis dassonvillei subsp. dassonvillei.
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Phycicoccus aerophilus sp. nov., isolated from air
A Gram-positive, short rod-shaped, non-spore-forming bacterium, designated 5516T-20T, was isolated from an air sample. The results of 16S rRNA gene sequence analyses showed that strain 5516T-20T belonged to the family Intrasporangiaceae, having the highest sequence similarities (97.5 and 96.1 %, respectively) with respect to the type strains of Phycicoccus dokdonensis and Phycicoccus jejuensis. The value for DNA–DNA hybridization between 5516T-20T and P. dokdonensis DS-8T was 41 %. Strain 5516T-20T contained menaquinone MK-8(H4) as the major isoprenoid quinone, possessed phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol as the polar lipids and contained glucose and ribose as whole-cell sugars. The major fatty acids were C17 : 1 ω8c, iso-C16 : 0, iso-C15 : 0, C17 : 0 and iso-C14 : 0. The G+C content of the DNA from strain 5516T-20T was 70.5 mol%. On the basis of the data from the polyphasic taxonomic study, strain 5516T-20T represents a novel species within the genus Phycicoccus, for which the name Phycicoccus aerophilus sp. nov. is proposed. The type strain is 5516T-20T (=KACC 20658T =DSM 18548T).
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Curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field
More LessA Gram-positive, non-motile, pale-yellow, short rod-shaped bacterium, strain DCY26T, was isolated from soil of a ginseng field in South Korea and was investigated to determine its taxonomic position. The organism grew optimally at 30–37 °C. The G+C content of its DNA was 65.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DCY26T was related most closely to species of the genus Curtobacterium, in the family Microbacteriaceae. Strain DCY26T showed highest 16S rRNA gene sequence similarity to Curtobacterium pusillum DSM 20527T (96.3 %), Curtobacterium luteum DSM 20542T (96.2 %), Curtobacterium flaccumfaciens LMG 3645T (96.2 %), Curtobacterium citreum DSM 20528T (96.1 %), Curtobacterium albidum DSM 20512T (96.1 %) and Curtobacterium herbarum DSM 14013T (95.3 %). The predominant menaquinone of strain DCY26T was MK-9. Other chemotaxonomic data also supported the affiliation of strain DCY26T to the genus Curtobacterium. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain DCY26T is considered to represent a novel species of the genus Curtobacterium, for which the name Curtobacterium ginsengisoli sp. nov. is proposed. The type strain is DCY26T (=KCTC 13163T =JCM 14773T).
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Mycobacterium arosiense sp. nov., a slowly growing, scotochromogenic species causing osteomyelitis in an immunocompromised child
A yellow-pigmented, scotochromogenic, slowly growing mycobacterial strain, designated T1921T, was isolated from the disseminated osteomyelitic lesions of a 7-year-old child with an underlying partial gamma interferon receptor alpha-1 deficiency. Hybridization by the line probe assay indicated the presence of a Mycobacterium species. Sequencing of the 16S rRNA gene, the internally transcribed spacer (ITS) region and the hsp65 and rpoB genes revealed that strain T1921T could be differentiated from all recognized species of the genus Mycobacterium. Phylogenetic analysis based on the 16S rRNA gene indicated that strain T1921T was related most closely to Mycobacterium intracellulare, whereas analysis based on the ITS and hsp65 and rpoB genes indicated that it was most closely related to Mycobacterium avium. Phenotypic tests were not able to differentiate strain T1921T from similar slowly growing mycobacteria. Strain T1921T is considered to represent a novel species of the genus Mycobacterium, for which the name Mycobacterium arosiense sp. nov. is proposed. The type strain is T1921T (=DSM 45069T =ATCC BAA-1401T).
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Phycicoccus bigeumensis sp. nov., a mesophilic actinobacterium isolated from Bigeum Island, Korea
More LessAn actinomycete strain, designated MSL-03T, was isolated from a soil sample collected from Bigeum Island in the Republic of Korea. The coccoid cells of the organism were Gram-positive and non-motile and did not form endospores. The colonies were yellow, small, circular with entire margins and contained meso-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The major menaquinone was MK-8(H4). The polar lipids detected were diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids were iso-C15 : 0, iso-C16 : 0, C17 : 0, anteiso-C15 : 0, 10-methyl C17 : 0, iso-C14 : 0 and C17 : 1 ω8c. The DNA G+C content was 73.4 mol%. Phylogenetic analyses based on the 16S rRNA gene sequence positioned strain MSL-03T in a clade comprising Phycicoccus jejuensis KSW2-15T and Phycicoccus dokdonensis DS-8T. The organism showed the highest level of sequence similarity with respect to P. dokdonensis DS-8T (99.58 % over 1424 nt), with differences in 64 positions out of 1488. The levels of DNA–DNA relatedness with its closest neighbours (<70 %) showed that the isolate represents a distinct genomic species. On the basis of the phenotypic and genotypic results, strain MSL-03T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus bigeumensis sp. nov. is proposed. The type strain is strain MSL-03T (=KCTC 19266T =DSM 19264T).
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Marihabitans asiaticum gen. nov., sp. nov., a meso-diaminopimelic acid-containing member of the family Intrasporangiaceae
More LessStrain HG667T, isolated from surface seawater collected at the Kesennuma ferry port in Miyagi Prefecture, Japan, was found to be a Gram-positive, catalase-positive bacterium comprising irregular short rods and cocci. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major menaquinone was MK-8(H4). Mycolic acids were not detected. The G+C content of the DNA was 70 mol%. Analysis of the 16S rRNA gene sequence revealed that the strain represents a novel lineage within the family Intrasporangiaceae, order Actinomycetales, being associated with the genus Kribbia. On the basis of morphological, biochemical and chemotaxonomic properties of the strain, together with phylogenetic data relating to the 16S rRNA gene sequence, HG667T represents a novel genus and species in the family Intrasporangiaceae, for which the name Marihabitans asiaticum gen. nov., sp. nov. is proposed. The type strain of Marihabitans asiaticum is HG667T (=MBIC07497T =DSM 18935T).
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Kineococcus gynurae sp. nov., isolated from a Thai medicinal plant
More LessA novel, Gram-positive, motile, coccus-shaped, orange-pigmented organism, designated strain KKD096T, was isolated from the roots of a Thai medicinal plant, Gynura pseudochina DC. var. hispida Thwaites. Growth of strain KKD096T occurred at temperatures of 14–34 °C, at pH 5.0–9.0 and at NaCl concentrations up to 7 % (w/v). Whole-cell hydrolysates contained arabinose and galactose as the characteristic sugars. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The glycan moiety of the murein contained acetyl residues. The predominant menaquinone was MK-9(H2); mycolic acids were not detected. The genomic DNA G+C content was 73.3 mol%. The major cellular fatty acid was anteiso-C15 : 0 (81.42 % of the total). Strain KKD096T was assigned to the genus Kineococcus on the basis of 16S rRNA gene sequence analysis; it was most closely related to Kineococcus radiotolerans DSM 14245T (97.1 % similarity). DNA–DNA hybridization revealed 39.4 % relatedness between these two taxa. On the basis of the genotypic and phenotypic data presented, strain KKD096T is considered to represent a novel species of the genus Kineococcus, for which the name Kineococcus gynurae sp. nov. is proposed. The type strain is KKD096T (=TISTR 1856T=NRRL B-24568T=BCC 26245T=NBRC 103943T).
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Actinopolymorpha rutila sp. nov., isolated from a forest soil
More LessA Gram-positive, aerobic actinomycete, designated strain YIM 45725T, was isolated from a forest soil sample in Yunnan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was a member of the family Nocardioidaceae and was related most closely to Actinopolymorpha singaporensis IM 7744T (99.4 % 16S rRNA gene sequence similarity). Chemotaxonomic data, including cell-wall components, menaquinones and polar lipids, supported the affiliation of strain YIM 45725T to the genus Actinopolymorpha. The results of physiological and biochemical investigations and DNA–DNA hybridization experiments demonstrated that strain YIM 45725T differed from the single recognized species of the genus Actinopolymorpha. Strain YIM 45725T is therefore considered to represent a novel species of the genus Actinopolymorpha, for which the name Actinopolymorpha rutila sp. nov. is proposed. The type strain is YIM 45725T (=CCTCC AA 206004T=DSM 18448T).
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- Archaea
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Halomicrobium katesii sp. nov., an extremely halophilic archaeon
Two extremely halophilic archaea, strains Al-5T and K-1, were isolated from Lake Tebenquiche (Atacama Saltern, Chile) and Ezzemoul sabkha (Algeria), respectively. Cells of the two strains were short-rod-shaped and Gram-negative; colonies were orange-pigmented. They grew optimally at 37–40 °C and pH 7.0–7.5 in the presence of 25 % (w/v) NaCl. Magnesium was not required. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerophosphate methyl ester, the absence of phosphatidylglycerosulfate, and the presence of sulfated diglycosyl diether and diether diglycosyl as the sole glycolipids. DNA G+C contents of strains Al-5T and K-1 were 52.4 and 52.9 mol% (T m method), respectively. 16S rRNA gene sequence comparison with database sequences showed that strains Al-5T and K-1 were most closely related to Halomicrobium mukohataei DSM 12286T (similarities of 97.5 and 96.9 %, respectively). DNA–DNA hybridization indicated that strains Al-5T and K-1 were members of a single species. However, DNA–DNA relatedness to Halomicrobium mukohataei was 55.7±2.5 %. A comparative analysis of phenotypic characteristics and DNA–DNA hybridization between the isolates and Halomicrobium mukohataei DSM 12286T supported the conclusion that Al-5T and K-1 represent a novel species within the genus Halomicrobium, for which the name Halomicrobium katesii sp. nov. is proposed. The type strain is Al-5T (=CECT 7257T=DSM 19301T).
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Natrinema gari sp. nov., a halophilic archaeon isolated from fish sauce in Thailand
Two Gram-negative, rod-shaped, halophilic archaea, designated strains HIS40-3T and HDS3-1, were isolated from anchovy fish sauce (nam-pla) collected from two different locations in Thailand. The two strains were able to grow at 20–60 °C (optimum 37–40 °C), at 1.7–5.1 M NaCl (optimum 2.6–3.4 M NaCl) and at pH 5.5–8.5 (optimum pH 6.0–6.5). Hypotonic treatment with less than 1.7 M NaCl caused cell lysis. The major polar lipids of the isolates were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, two glycolipids and one unidentified lipid. The DNA G+C contents were 64.0–65.4 mol%. In addition to phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strains HIS40-3T and HDS3-1 were related most closely to species of the genus Natrinema. Levels of 16S rRNA gene sequence similarity between strains HIS40-3T and HDS3-1 and the type strains of recognized Natrinema species were 99.1–96.6 %. The two novel strains could be distinguished from recognized Natrinema species on the basis of low levels of DNA–DNA relatedness and differences in whole-cell protein patterns and phenotypic properties. Levels of 16S rRNA gene sequence similarity and DNA–DNA relatedness between the two strains were 99.7 and 77.7 %, respectively, suggesting that they should be classified as representing a single species. Based on these taxonomic data, strains HIS40-3T and HDS3-1 are considered to represent a novel species of the genus Natrinema, for which the name Natrinema gari sp. nov. is proposed. The type strain is HIS40-3T (=BCC 24370T =JCM 14663T =PCU 303T).
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- Bacteroidetes
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Flavobacterium resistens sp. nov., isolated from stream sediment
More LessAn aerobic, yellow-pigmented, Gram-negative bacterium, designated strain BD-b365T, was isolated from sediment of the Hakjang stream in Busan, South Korea. Growth was observed at 15–40 °C (optimum 20–30 °C) and at pH 6.0–9.5 (optimum pH 7.0–8.0). Cells were non-spore-forming rods that showed gliding motility and contained branched and hydroxy fatty acids. The G+C content of the genomic DNA was 35.4 mol%. The major respiratory quinone was menaquinone-6 (MK-6). The major polar lipid of strain BD-b365T was phosphatidylethanolamine. Comparative 16S rRNA gene sequence analysis showed that strain BD-b365T formed a distinct phyletic line within the genus Flavobacterium. Based on levels of 16S rRNA gene sequence similarity, the novel strain was related most closely to Flavobacterium aquidurense WB 1.1-56T, but the level of DNA–DNA relatedness between these two strains was only 9.6 %. On the basis of phenotypic and genotypic data, it is clear that strain BD-b365T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium resistens sp. nov. is proposed. The type strain is BD-b365T (=KCTC 22078T =DSM 19382T).
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Eudoraea adriatica gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae
More LessA novel aerobic, non-motile, Gram-negative bacterium, designated AS06/20aT, was isolated from coastal waters of the Adriatic Sea and subjected to polyphasic taxonomic analysis. Cells were rod-shaped and formed non-pigmented punctiform colonies on agar plates. The novel isolate grew heterotrophically on a variety of carbon compounds, including organic acids, carbohydrates, polyols, amino acids and complex organic substrates. Cells grew at 15–33 °C (optimum 30 °C), pH 6.5–8.5 (optimum pH 7.5–8.0) and between 2 and 6 % (w/v) NaCl (optimum 2 %). The dominant fatty acids (>5 %) detected in strain AS06/20aT were iso-C15 : 1 G, iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 1 ω9c and iso-C17 : 0 3-OH. The major respiratory quinone was MK-6. The G+C content of the genomic DNA was 38.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AS06/20aT fell within the family Flavobacteriaceae in the phylum Bacteroidetes. Comparative 16S rRNA gene sequence analysis indicated that strain AS06/20aT was most closely related to members of the genera Zeaxanthinibacter and Robiginitalea, sharing 92–93 % 16S rRNA gene sequence similarity with representatives of these genera. It is proposed, from the results of the polyphasic taxonomic analysis, that the strain should be placed in a new genus, Eudoraea gen. nov., with Eudoraea adriatica sp. nov. as the type species. The type strain is AS06/20aT (=DSM 19308T=CIP 109577T=OOB 358T).
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Bizionia argentinensis sp. nov., isolated from surface marine water in Antarctica
A marine bacterial strain, designated strain JUB59T, was isolated from surface seawater in Antarctica and subsequently characterized. Cells were found to be Gram-negative, non-motile rods forming butyrous, shiny, yellowish orange colonies on marine agar. Growth occurred at 2–28 °C (optimally at 22–25 °C) but not at 30 °C; Na+ ions were required, but 9 % NaCl (w/v) was not tolerated. Phylogenetic analysis, based on comparisons of the complete 16S rRNA gene sequence of the novel isolate with the sequences of closely related strains, showed that strain JUB59T belonged to the family Flavobacteriaceae, representing a novel species of the genus Bizionia. The highest levels of sequence similarity were found with respect to Bizionia myxarmorum ADA-4T (97.4 %) and Bizionia algoritergicola APA-1T (97.1 %). However, the DNA–DNA relatedness of strain JUB59T with respect to these two strains was low (15.9–17.3 and 19.3–22.1 %, respectively). The predominant fatty acids of strain JUB59T were iso-15 : 1ω10c (18.1 %), iso-15 : 0 (17.3 %), anteiso-15 : 0 (13.9 %), iso-17 : 0 3-OH (9.2 %), 15 : 0 (6.0 %) and iso-16 : 0 3-OH (5.3 %). The main polar lipids were phosphatidylethanolamine, an aminolipid, an amino-positive phospholipid and two unidentified lipids. MK-6 was the major respiratory quinone (>90 %) and the DNA G+C content was 34 mol%. On the basis of the data obtained, strain JUB59T represents a novel species of the genus Bizionia, for which the name Bizionia argentinensis sp. nov. is proposed. The type strain is JUB59T (=DSM 19628T=CCM-A-29 1259T).
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- Other Bacteria
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Leptospira weilii serovar Topaz, a new member of the Tarassovi serogroup isolated from a bovine source in Queensland, Australia
This paper reports on a Leptospira isolate of bovine origin and its identification as belonging to a previously unknown serovar, for which the name Topaz is proposed. The isolate (94-79970/3) was cultured from bovine urine from a north Queensland dairy farm in Australia. Strain 94-79970/3 grew at 30 °C in Ellinghausen McCullough Johnson Harris (EMJH) medium but failed to grow at 13 °C in EMJH medium or in the presence of 8-azaguanine. Serologically, strain 94-79970/3 produced titres against the Leptospira borgpetersenii serovar Tarassovi, the reference strain for the Tarassovi serogroup; however, no significant titres to any other serovars within the serogroup were obtained. Using 16S rRNA and DNA gyrase subunit B gene analysis, strain 94-79970/3 was identified as a member of the species Leptospira weilii. We propose that the serovar be named Topaz, after the location where the original isolate was obtained. The reference strain for this serovar is 94-79970/3 (=KIT 94-79970/3=LT722).
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Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochaete isolated from a deep-sea hydrothermal vent chimney
A novel thermophilic, anaerobic bacterium, strain RASENT, was isolated from a deep-sea hydrothermal vent chimney sample collected in the Iheya North field, Okinawa Trough, Japan, at a water depth of 982 m. The cells were motile, Gram-negative and helical with hooked ends, 0.23–0.28×15–27 μm in size with an approximate wavelength of 1.1–1.5 μm. Growth of the strain was observed at 37–60 °C (optimum 50 °C), in 2.5–3.5 % (w/v) NaCl (optimum 2.5–3 % NaCl) and at pH 6.0–7.5 (optimum pH 7.0). The strain grew on yeast extract only of the substrates examined in this study. The G+C content of the genomic DNA was 27.1 mol%. Major fatty acids for the strain were C16 : 0, C18 : 1(Δ9) trans, C18 : 0 and C18 : 1(Δ9) cis. Based on comparative 16S rRNA gene sequence analysis, strain RASENT formed a deeply branching lineage within the phylum Spirochaetes and had only low levels of sequence similarity with other species of the phylum (range of similarity 72.1–80.6 %). Hence, we propose the name Exilispira thermophila gen. nov., sp. nov. The type strain of Exilispira thermophila is strain RASENT (=JCM 14728T =NBRC 103205T =KCTC 5595T).
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Leptospira wolffii sp. nov., isolated from a human with suspected leptospirosis in Thailand
A single Leptospira strain (designated Khorat-H2T) was isolated from the urine of an adult male patient with suspected leptospirosis from the province of Nakornrachasima, Thailand. The isolate showed typical Leptospira motility and morphology under dark-field microscopy. Cells were 10–13 μm long and 0.2 μm in diameter, with a wavelength of 0.5 μm and an amplitude of approximately 0.3 μm. Phenotypically, strain Khorat-H2T did not grow at 13 °C but grew at 30 and 37 °C and in the presence of 8-azaguanine. Serological identification using the microscopic agglutination test revealed that strain Khorat-H2T had no cross-reaction with any recognized Leptospira serogroups. Phylogenetic analysis of the 16S rRNA gene sequence placed the novel strain within the radiation of the genus Leptospira, with sequence similarities of 88.1–97.7 % to recognized Leptospira species. DNA–DNA hybridization against the type strains of the three most closely related Leptospira species was used to confirm the results of the 16S rRNA sequence analysis. The G+C content of strain Khorat-H2T was 41.8 mol%. On the basis of phenotypic, serological and phylogenetic data, strain Khorat-H2T represents a novel species of the genus Leptospira, for which the name Leptospira wolffii sp. nov. is proposed. The type strain is Khorat-H2T (=WHO LT1686T =KIT Khorat-H2T).
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Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge
The taxonomic positions of two environmental isolates from South Korea were established using a combination of genotypic and phenotypic data. The organisms, designated PB314T and Ho-08T, were Gram-negative, rod-shaped and non-spore-forming and had chemotaxonomic properties consistent with their classification in the genus Deinococcus 16S rRNA gene tree, the highest sequence similarities being shown to the type strains of Deinococcus grandis (96.3–96.7 %) and Deinococcus indicus (96.3–96.4 %). The isolates shared relatively high 16S rRNA gene sequence similarity (98.1 %) but had a DNA–DNA relatedness value of only 22 %. Chemotaxonomic data revealed that both strains possess quinone system MK-8 as the predominant compound, C16 : 1 ω7c and C16 : 0 as major fatty acids and ornithine as a diamino acid in the peptidoglycan structure, corroborating our assignment of the strains to the genus Deinococcus. The results of phylogenetic analyses based on 16S rRNA gene sequences, DNA–DNA relatedness values and physiological and biochemical tests clearly demonstrated that the two strains represent distinct species. On the basis of these data, two novel species, Deinococcus aquaticus sp. nov. (type strain PB314T =KCTC 12552T =NBRC 101311T) and Deinococcus caeni sp. nov. (type strain Ho-08T =KCTC 12553T =NBRC 101312T), are proposed.
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