- Volume 59, Issue 4, 2009
Volume 59, Issue 4, 2009
- New Taxa
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- Proteobacteria
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Paracoccus aestuarii sp. nov., isolated from tidal flat sediment
A Gram-negative micro-organism, designated strain B7T, was isolated from tidal flat sediment and subjected to a polyphasic taxonomic study involving morphological, physiological, biochemical and 16S rRNA gene sequence analyses. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain B7T belonged to the genus Paracoccus and was closely related phylogenetically to Paracoccus marcusii MH1T (97.5 % sequence similarity), Paracoccus marinus KKL-A5T (97.5 %), Paracoccus haeundaensis BC74171T (97.3 %), Paracoccus carotinifaciens E-396T (97.3 %), Paracoccus homiensis DD-R11T (97.2 %), Paracoccus seriniphilus MBT-A4T (96.9 %) and other type strains of the genus Paracoccus (95.2–96.7 %). The G+C content of the genomic DNA and the major isoprenoid quinone of the type strain were 62.0 mol% and ubiquinone-10, respectively. The major fatty acid components were C18 : 1 ω7c (68.9 %) and C18 : 0 (18.1 %); this profile, with C18 : 1 ω7c as the predominant fatty acid, was characteristic of members of the genus Paracoccus. The 16S rRNA gene sequence analysis, DNA–DNA hybridization studies and physiological and biochemical tests identified genotypic and phenotypic differences between strain B7T and recognized Paracoccus species. On the basis of these data, therefore, strain B7T represents a novel species of the genus Paracoccus, for which the name Paracoccus aestuarii sp. nov. is proposed. The type strain is B7T (=KCTC 22049T=DSM 19484T=JCM 15119T).
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Devosia albogilva sp. nov. and Devosia crocina sp. nov., isolated from a hexachlorocyclohexane dump site
More LessTwo bacterial strains, IPL15T and IPL20T, isolated from a hexachlorocyclohexane dump site in India, were characterized by using a polyphasic approach. Based on 16S rRNA gene sequence analysis, both strains belonged to the genus Devosia; highest sequence similarities of strain IPL15T were observed with Devosia neptuniae J1T and Devosia geojensis BD-c194T (96.2 % in each case) and the highest sequence similarity of strain IPL20T was observed with Devosia soli GH2-10T (98.6 %). Phylogenetic analysis showed the distinct lineages of strains IPL15T and IPL20T among members of the genus Devosia. The presence of C18 : 0 3-OH and C10 : 0 3-OH fatty acids supported their respective positions within the genus Devosia. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization results, it is concluded that strains IPL15T and IPL20T represent two distinct species of the genus Devosia for which the names Devosia albogilva sp. nov. and Devosia crocina sp. nov., respectively, are proposed. The type strains are Devosia albogilva IPL15T (=CCM 7427T=MTCC 8594T) and Devosia crocina IPL20T (=CCM 7425T=MTCC 8590T).
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Nautella italica gen. nov., sp. nov., isolated from a marine electroactive biofilm
Five isolates obtained from a marine electroactive biofilm grown on a stainless steel cathode were investigated by using a polyphasic taxonomic approach. Analyses of whole-cell fatty acid methyl esters and 16S rRNA gene sequences showed that the isolates belonged to the Roseobacter lineage of the class Alphaproteobacteria. Both phenotypic and genotypic analyses demonstrated that the five new isolates constituted a single species that did not represent a recognized member of the Roseobacter lineage. Therefore the five isolates represent a novel genus and species, for which the name Nautella italica gen. nov., sp. nov. is proposed. The type strain is LMG 24365T (=CCUG 55857T). The DNA G+C content of the type strain is 61 mol%.
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Pelagibius litoralis gen. nov., sp. nov., a marine bacterium in the family Rhodospirillaceae isolated from coastal seawater
More LessA Gram-negative, strictly aerobic, slightly curved rod-shaped bacterial strain, designated CL-UU02T, was isolated from coastal seawater off the east coast of Korea. 16S rRNA gene sequence analysis revealed a clear affiliation of this novel strain with the family Rhodospirillaceae. Strain CL-UU02T formed a robust cluster with the type strains of species of the genus Rhodovibrio at 16S rRNA gene sequence similarity levels of 89.9–90.4 %. Strain CL-UU02T shared no more than 89 % 16S rRNA gene sequence similarity with the type strains of other species in the family Rhodospirillaceae. Strain CL-UU02T was able to grow in the presence of 2–6 % sea salts, and grew optimally at 28–30 °C and pH 7–8. The DNA G+C content of strain CL-UU02T was 66.3 mol%. On the basis of phylogenetic analyses and chemotaxonomic and physiological data, strain CL-UU02T is considered to represent a novel species of a new genus in the family Rhodospirillaceae, for which the name Pelagibius litoralis gen. nov., sp. nov. is proposed. The type strain of Pelagibius litoralis is CL-UU02T (=KCCM 42323T=JCM 15426T).
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Mesorhizobium metallidurans sp. nov., a metal-resistant symbiont of Anthyllis vulneraria growing on metallicolous soil in Languedoc, France
A polyphasic taxonomic approach was used to characterize 31 rhizobial isolates obtained from Anthyllis vulneraria, a metallicolous legume species, growing close to a zinc mine in the south of France (Saint Laurent le Minier). Comparative analysis of nearly full-length 16S rRNA gene sequences showed that these Gram-negative bacteria belonged to the genus Mesorhizobium and that they were related most closely to Mesorhizobium tianshanense ORS 2640T. The phylogenetic relationships of these isolates with other Mesorhizobium species were confirmed by sequencing and analysis of the recA and atpD genes, which were used as alternative chromosomal markers. These novel mesorhizobial strains tolerated high concentrations of heavy metals: 16–32 mM Zn and 0.3–0.5 mM Cd. DNA–DNA hybridizations revealed >73 % relatedness between the strains isolated from A. vulneraria, but only 19–33 % relatedness between these and the type strains of M. tianshanense and Mesorhizobium mediterraneum. These results, together with other phenotypic characteristics, support the conclusion that these isolates represent a single, novel species of the genus Mesorhizobium, for which the name Mesorhizobium metallidurans sp. nov. is proposed. The type strain is STM 2683T (=CFBP 7147T=LMG 24485T).
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Salinivibrio siamensis sp. nov., from fermented fish (pla-ra) in Thailand
A Gram-negative, facultatively anaerobic, moderately halophilic bacterium, strain ND1-1T, was isolated from fermented fish (pla-ra) in Thailand. The cells were curved rods, motile and non-endospore-forming. The novel strain grew optimally at 37 °C, at pH 8 and in the presence of 9−10 % (w/v) NaCl. The predominant respiratory lipoquinone was Q-8. The major cellular fatty acids were C16 : 0 and C12 : 0. Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 49.0 mol%. Comparative 16S rRNA gene sequence analyses indicated that strain ND1-1T was closely related to Salinivibrio costicola, which comprises three subspecies, and Salinivibrio proteolyticus with gene sequence similarities of 98.3–98.6 %. Strain ND1-1T showed low levels of DNA–DNA relatedness with S. costicola subsp. costicola JCM 15095T (33.2 %), S. costicola subsp. alcaliphilus DSM 16359T (38.4 %), S. costicola subsp. vallismortis JCM 15096T (59.7 %), and S. proteolyticus AF-2004T (42.1 %). On the basis of the physiological and biochemical characteristics and the molecular data presented, strain ND1-1T should be classified as a novel species of the genus Salinivibrio for which the name Salinivibrio siamensis sp. nov. is proposed. The type strain is ND1-1T (=JCM 14472T=PCU 301T=TISTR 1810T).
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Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment
More LessA Gram-negative, arsenate-respiring and arsenite-oxidizing marine bacterium, NKSG1T, was isolated from hydrothermal sediment at Santorini, Greece. Strain NKSG1T was a facultatively anaerobic, motile, non-spore-forming, rod-shaped bacterium. Growth occurred optimally at 35–40 °C, between pH 5.5 and 9.0 and with 0.5–16 % NaCl. Energy was conserved by the aerobic oxidation of a range of complex substrates, carbohydrates and organic acids, or anaerobically by arsenate reduction, nitrate reduction coupled to the oxidation of organic carbon or lactate fermentation. Oxidation of arsenite and anaerobic nitrate-dependent oxidation of Fe(II) were facilitated by the presence of an organic carbon source. The DNA G+C content was 58.1 mol%. The major respiratory quinone was Q-9. The significant fatty acids were 16 : 1ω9c, summed feature 3 (iso-15 : 0 2-OH/16 : 1ω7c), 16 : 0 and 18 : 1ω9c. Analysis of 16S rRNA gene sequences showed that strain NKSG1T fits within the phylogenetic cluster of the genus Marinobacter and is most closely related to Marinobacter koreensis DD-M3T (99.3 % similarity). The degree of relatedness with M. koreensis DSM 17924T based on DNA–DNA hybridization was 56 %. The results of a polyphasic study indicated that strain NKSG1T is a representative of a novel species within the genus Marinobacter, for which the name Marinobacter santoriniensis sp. nov. is proposed. The type strain is NKSG1T (=DSM 21262T =NCIMB 14441T=ATCC BAA-1649T). The capacity for arsenic reduction or oxidation has not been demonstrated previously for this genus.
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- Eukaryotic Micro-Organisms
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Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera
More LessMixed trypanosomatid infections (a simultaneous presence of two or more parasites in the same host) have long been suspected to represent an obstacle for recovering cultures that would faithfully represent original species descriptions. However, without the means to directly compare the parasites in the host and in culture, this would remain just a possibility. Here we have used PCR-based genotyping of spliced leader RNA gene repeats to analyse several novel species of insect trypanosomatids isolated from heteropteran hosts and to compare them with the parasites that had been detected in the gut smears of the same hosts. We have found that, whereas the original infections were dominated by some blastocrithidia-like parasites, most of the respective axenic cultures contained novel species of Crithidia and Leptomonas. Therefore, we concluded that, in each case, this replacement was caused by differences in cultivation properties between the original predominant blastocrithidia and the less fastidious parasite that was later recovered in culture. The properties of the new organisms, including their morphology and ultrastructure, as well as their phylogenetic affinities within the family, were investigated and used to describe five novel species.
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Cryptococcus bromeliarum sp. nov., an orange-coloured basidiomycetous yeast isolated from bromeliads in Brazil
More LessDuring a survey of yeasts associated with the phylloplane of several bromeliad species in Itapuã Park in southern Brazil, we isolated four orange-coloured strains which were found to represent a novel anamorphic tremellaceous (Tremellales, Agaricomycotina, Basidiomycota) yeast species, Cryptococcus bromeliarum sp. nov. (type strain BI20T =CBS 10424T =NRRL Y-48112T). PCR-fingerprinting profiles of the four strains with primers M13 and (GTG)5 were almost identical, which suggested conspecificity among the isolates. On the basis of D1/D2 26S rDNA sequence analysis, C. bromeliarum is phylogenetically closely related to other orange-coloured Cryptococcus species, namely Cryptococcus armeniacus, C. amylolyticus, C. tibetensis and C. cistialbidi, but differed from these species by at least six nucleotide substitutions and was thus considered a separate species. Physiological differences from C. armeniacus, C. amylolyticus and C. cistialbidi included the inability of C. bromeliarum to assimilate citrate and to form starch-like compounds. Differentiation from C. tibetensis can be achieved by the ability of the latter to assimilate ethylamine.
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Jaminaea angkorensis gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales
More LessIn the course of molecular characterization of yeasts isolated from decaying leaves collected in Cambodia, anamorphic yeast strains were detected that clustered with the Microstromatales in molecular phylogenetic analyses of the chromosomal regions coding for the D1/D2 domain of the large-subunit 26S rRNA gene, the small-subunit 18S rRNA gene and the internal transcribed spacer ITS1-5.8S rRNA gene-ITS2. In the trees obtained, the isolates formed a distinct, basal branch of Microstromatales supported with high bootstrap values. As the isolates could not be identified with any known genus, we have described them as Jaminaea angkorensis gen. et sp. nov. (type strain C5bT=CBS 10918T=CCY 88-1-1T). In the chromosomal region coding for the 18S rRNA gene, the type strain of the novel species contained a group IB intron that was similar in location and sequence to introns found in certain species of Exobasidiales and Entylomatales. As no similar introns have been detected in Microstromatales, the new genus might represent a phylogenetic link connecting these three orders.
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- International Committee On Systematics Of Prokaryotes
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- Minutes
Volumes and issues
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Volume 74 (2024)
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Volume 73 (2023)
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Volume 72 (2022 - 2023)
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Volume 71 (2020 - 2021)
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Volume 70 (2020)
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Volume 69 (2019)
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Volume 68 (2018)
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Volume 67 (2017)
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Volume 66 (2016)
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Volume 65 (2015)
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Volume 64 (2014)
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Volume 63 (2013)
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Volume 62 (2012)
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Volume 61 (2011)
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Volume 60 (2010)
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Volume 59 (2009)
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Volume 58 (2008)
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Volume 57 (2007)
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Volume 56 (2006)
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Volume 55 (2005)
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Volume 54 (2004)
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Volume 53 (2003)
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Volume 52 (2002)
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Volume 51 (2001)
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Volume 50 (2000)
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Volume 49 (1999)
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Volume 48 (1998)
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Volume 47 (1997)
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Volume 46 (1996)
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Volume 45 (1995)
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Volume 44 (1994)
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Volume 43 (1993)
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Volume 42 (1992)
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Volume 41 (1991)
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Volume 40 (1990)
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Volume 39 (1989)
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Volume 38 (1988)
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Volume 37 (1987)
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Volume 36 (1986)
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Volume 35 (1985)
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Volume 34 (1984)
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Volume 33 (1983)
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Volume 32 (1982)
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Volume 31 (1981)
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Volume 30 (1980)
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Volume 29 (1979)
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Volume 28 (1978)
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Volume 27 (1977)
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Volume 26 (1976)
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Volume 25 (1975)
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Volume 24 (1974)
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Volume 23 (1973)
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Volume 22 (1972)
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Volume 21 (1971)
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Volume 20 (1970)
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Volume 19 (1969)
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Volume 18 (1968)
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Volume 17 (1967)
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Volume 16 (1966)
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Volume 15 (1965)
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Volume 14 (1964)
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Volume 13 (1963)
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Volume 12 (1962)
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Volume 11 (1961)
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Volume 10 (1960)
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Volume 9 (1959)
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Volume 8 (1958)
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Volume 7 (1957)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)