- Volume 59, Issue 6, 2009
Volume 59, Issue 6, 2009
- New Taxa
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- Proteobacteria
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Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan
More LessTwo novel Psychrobacter-like bacteria, strains KC 40T and KC 65, were isolated from a marine crustacean specimen collected from the Sea of Japan, and were characterized by using a polyphasic approach. Strains were selected on the basis of their ability to produce black–brown diffusible pigments on commonly used organic media, which appears to be a unique characteristic of recognized members of the genus Psychrobacter. Phylogenetic analyses based on both 16S rRNA and gyrB gene sequences showed that the novel isolates formed a separate cluster within the genus Psychrobacter. Strains KC 40T and KC 65 shared highest levels of 16S rRNA gene sequence similarity with Psychrobacter urativorans DSM 14009T (98.0 %), Psychrobacter pulmonis CCUG 46240T (97.9 %), Psychrobacter cibarius JG-219T (97.9 %), Psychrobacter faecalis Iso-46T (97.8 %), Psychrobacter aquimaris SW-210T (97.6 %), Psychrobacter namhaensis SW-242T (97.6 %) and Psychrobacter nivimaris 88/2-7T (97.6 %). DNA–DNA hybridization experiments revealed 84 % DNA–DNA relatedness between strains KC 40T and KC 65 but much lower levels of relatedness (7–35 %) between the novel strains and the type strains of recognized Psychrobacter species, confirming their assignment to a single novel species of the genus Psychrobacter. The two novel strains could be distinguished from recognized species of the genus Psychrobacter based on a combination of physiological and biochemical characteristics. On the basis of phenotypic and molecular properties, strains KC 40T and KC 65 are considered to represent a novel species of the genus Psychrobacter, for which the name Psychrobacter fulvigenes sp. nov. is proposed. The type strain is KC 40T (=KMM 3954T=NRIC 0746T=JCM 15525T).
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Salinisphaera hydrothermalis sp. nov., a mesophilic, halotolerant, facultatively autotrophic, thiosulfate-oxidizing gammaproteobacterium from deep-sea hydrothermal vents, and emended description of the genus Salinisphaera
A mesophilic, aerobic, facultatively chemolithoautotrophic bacterium, designated strain EPR70T, was isolated from hydrothermal fluids from diffuse-flow vents on the East Pacific Rise at ° 50′ N 10 ° 17′ W. Cells were Gram-negative rods, approximately 0.8–1.0 μm long and 0.3–0.5 μm wide. Strain EPR70T grew at 20–40 °C (optimum 30–35 °C), 1–25 % NaCl (optimum 2.5 %) and pH 5.0–7.5 (optimum pH 5.5). The shortest generation time observed for strain EPR70T was 42 min. Growth occurred under aerobic chemolithoautotrophic conditions in the presence of thiosulfate and CO2. Strain EPR70T grew heterotrophically with acetate or n-alkanes as sole carbon and energy sources, and in complex artificial seawater medium. Nitrate was not used as an electron acceptor. The G+C content of the genomic DNA was 64 mol%. Phylogenetic analysis of the 16S rRNA gene indicated that this organism is a member of the class Gammaproteobacteria, with Salinisphaera shabanensis E1L3AT as its closest relative (94 % sequence similarity). On the basis of phylogenetic analyses based on 16S rRNA, rbcL and alkB genes and physiological analysis, it is proposed that the organism represents a novel species within the genus Salinisphaera, for which the name Salinisphaera hydrothermalis sp. nov. is proposed. The type strain is EPR70T (=DSM 21483T =JCM 15514T).
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Phaselicystis flava gen. nov., sp. nov., an arachidonic acid-containing soil myxobacterium, and the description of Phaselicystidaceae fam. nov.
More LessA bacterial strain designated SBKo001T was isolated from a forest soil sample from Mt Makiling in Laguna, Philippines. It shows the general characteristics associated with myxobacteria, such as swarming of Gram-negative, rod-shaped vegetative cells, fruiting body formation and bacteriolytic activity. The strain is mesophilic, strictly aerobic and chemoheterotrophic and also exhibits resistance to various antibiotics. Major fatty acids are iso-C15 : 0, C17 : 1 2-OH and C20 : 4 (arachidonic acid). The G+C content of the genomic DNA is 69.2 mol%. A reference strain, NOSO-1 (=DSM 53757), isolated from the Etosha Basin in Namibia, shares nearly the same characteristics with SBKo001T. The identical 16S rRNA gene sequences of the two strains show 94 % identity to strains of the cellulose-degrading Byssovorax and Sorangium species. Phylogenetic analysis reveals a novel branch diverging from the Polyangiaceae, Sorangiineae, Myxococcales. Their uniqueness in morphological growth stages, unusual fatty acid profile, broad-spectrum antibiotic resistance and branch divergence from the Polyangiaceae imply that strains SBKo001T and NOSO-1 not only represent a novel genus and species, proposed here as Phaselicystis flava gen. nov., sp. nov., but also belong to a new family, Phaselicystidaceae fam. nov. The type strain of Phaselicystis flava is SBKo001T (=DSM 21295T =NCCB 100230T).
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Skermanella xinjiangensis sp. nov., isolated from the desert of Xinjiang, China
Strain 10-1-101T, isolated from a sand sample collected from the desert of Xinjiang, China, was a Gram-negative, aerobic, motile, rod-shaped bacterium. Colonies grown on 0.1× trypticase soy broth agar were circular, convex and light-pink-coloured. The major cellular fatty acids of the novel strain were C18 : 1 ω7c (72.71 %) and C16 : 0 (7.05 %). The DNA G+C content of strain 10-1-101T was 68.8 mol% and Q-10 was the major respiratory quinone. 16S rRNA gene sequence analysis indicated that strain 10-1-101T was related to type strains of the genus Skermanella, with sequence similarity values of 94.07 % with Skermanella aerolata DSM 18479T and 92.74 % with Skermanella parooensis DSM 9527T. On the basis of genotypic, phenotypic and phylogenetic data, this new strain represents a novel species of the genus Skermanella, for which the name Skermanella xinjiangensis sp. nov. is proposed. The type strain is 10-1-101T (=CCTCC AB 207153T=NRRL B-51273T).
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- Eukaryotic Micro-Organisms
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Trichomonascus apis sp. nov., a heterothallic yeast species from honeycomb
More LessFour strains of a novel heterothallic yeast species were isolated from pollen-storing cells of a honeycomb of honeybee (Apis mellifera) in Hungary. Analysis of the D1/D2 domain of the large-subunit (26S) rRNA gene sequences placed the strains in the Trichomonascus clade. The four strains share identical D1/D2 sequences and differ by 24 substitutions and nine indels from the genetically most closely related species, Blastobotrys attinorum. The name Trichomonascus apis sp. nov. is proposed for the novel species. The carbon-source assimilation spectrum of T. apis sp. nov. is rather broad. Unlike B. attinorum, it assimilates sucrose, trehalose, d-glucuronate and succinate and does not grow at 37 °C, thus enabling the two taxa to be distinguished. The type and isotype strains of Trichomonascus apis are NCAIM Y.01848T (=CBS 10922T =NRRL Y-48475T) and NCAIM Y.01849IT (=CBS 10923IT =NRRL Y-48476IT), respectively.
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- Evolution, Phylogeny And Biodiversity
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Phylogenetic analysis of Mycobacterium aurum and Mycobacterium neoaurum with redescription of M. aurum culture collection strains
More LessWe examined American Type Culture Collection (ATCC) strains of Mycobacterium aurum and Mycobacterium neoaurum by using multilocus DNA target sequencing. Apart from the type strain, all 10 ATCC M. aurum strains examined were classified incorrectly, with most being reclassified as belonging to the M. neoaurum–‘Mycobacterium lacticola’ relatedness group. All four M. neoaurum strains were tightly clustered, but heterogeneity was observed within the cluster. As a result of the incorrect annotation of the M. aurum strains, two commonly used methods of identification are compromised and two case reports implicating M. aurum as a human pathogen are probably incorrect, with the isolates probably belonging to the M. neoaurum–‘M. lacticola’ relatedness group. These findings together with a review of isolates identified at two large reference laboratories suggest that M. aurum is not a clinically significant isolate.
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Volumes and issues
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Volume 74 (2024)
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Volume 73 (2023)
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Volume 72 (2022 - 2023)
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Volume 71 (2020 - 2021)
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Volume 70 (2020)
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Volume 69 (2019)
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Volume 68 (2018)
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Volume 67 (2017)
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Volume 66 (2016)
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Volume 65 (2015)
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Volume 64 (2014)
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Volume 63 (2013)
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Volume 62 (2012)
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Volume 61 (2011)
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Volume 60 (2010)
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Volume 59 (2009)
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Volume 58 (2008)
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Volume 57 (2007)
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Volume 56 (2006)
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Volume 55 (2005)
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Volume 54 (2004)
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Volume 53 (2003)
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Volume 52 (2002)
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Volume 51 (2001)
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Volume 50 (2000)
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Volume 49 (1999)
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Volume 48 (1998)
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Volume 47 (1997)
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Volume 46 (1996)
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Volume 45 (1995)
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Volume 44 (1994)
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Volume 43 (1993)
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Volume 42 (1992)
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Volume 41 (1991)
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Volume 40 (1990)
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Volume 39 (1989)
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Volume 38 (1988)
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Volume 37 (1987)
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Volume 36 (1986)
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Volume 35 (1985)
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Volume 34 (1984)
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Volume 33 (1983)
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Volume 32 (1982)
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Volume 31 (1981)
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Volume 30 (1980)
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Volume 29 (1979)
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Volume 28 (1978)
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Volume 27 (1977)
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Volume 26 (1976)
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Volume 25 (1975)
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Volume 24 (1974)
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Volume 23 (1973)
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Volume 22 (1972)
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Volume 21 (1971)
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Volume 20 (1970)
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Volume 19 (1969)
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Volume 18 (1968)
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Volume 17 (1967)
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Volume 16 (1966)
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Volume 15 (1965)
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Volume 14 (1964)
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Volume 13 (1963)
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Volume 12 (1962)
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Volume 11 (1961)
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Volume 10 (1960)
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Volume 9 (1959)
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Volume 8 (1958)
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Volume 7 (1957)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)