- Volume 61, Issue 11, 2011
Volume 61, Issue 11, 2011
- Validation List
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- 142
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List of new names and new combinations previously effectively, but not validly, published
The purpose of this announcement is to effect the valid publication of the following effectively published new names and new combinations under the procedure described in the Bacteriological Code (1990 Revision). Authors and other individuals wishing to have new names and/or combinations included in future lists should send three copies of the pertinent reprint or photocopies thereof, or an electronic copy of the published paper, to the IJSEM Editorial Office for confirmation that all of the other requirements for valid publication have been met. It is also a requirement of IJSEM and the ICSP that authors of new species, new subspecies and new combinations provide evidence that types are deposited in two recognized culture collections in two different countries. It should be noted that the date of valid publication of these new names and combinations is the date of publication of this list, not the date of the original publication of the names and combinations. The authors of the new names and combinations are as given below, and these authors’ names will be included in the author index of the present issue. Inclusion of a name on these lists validates the publication of the name and thereby makes it available in bacteriological nomenclature. The inclusion of a name on this list is not to be construed as taxonomic acceptance of the taxon to which the name is applied. Indeed, some of these names may, in time, be shown to be synonyms, or the organisms may be transferred to another genus, thus necessitating the creation of a new combination.
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- Notification List
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Notification that new names and new combinations have appeared in volume 61, part 8, of the IJSEM
This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed according to the Rules of priority (i.e. page number and order of valid publication of names in the original articles). Taxonomic opinions included in this List (i.e. the creation of synonyms or the emendation of circumscriptions) cannot be considered as validly published nor, in any other way, approved by the International Committee on Systematics of Prokaryotes and its Judicial Commission.
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- New Taxa
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- Proteobacteria
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Methylogaea oryzae gen. nov., sp. nov., a mesophilic methanotroph isolated from a rice paddy field
More LessA novel methanotroph, designated strain E10T, was isolated from a rice paddy field in Uruguay. Strain E10T grew on methane and methanol as sole carbon and energy sources. Cells were Gram-negative, non-motile, non-pigmented, slightly curved rods showing type I intracytoplasmic membranes arranged in stacks. The strain was neutrophilic and mesophilic; optimum growth occurred at 30–35 °C with no growth above 37 °C. The strain possessed only a particulate methane monooxygenase (pmoA). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was most closely related to the moderately thermophilic strains Methylocaldum szegediense OR2T (91.6 % sequence similarity) and Methylococcus capsulatus Bath (91.5 %). Comparative sequence analysis of pmoA genes also confirmed that strain E10T formed a new lineage among the genera Methylocaldum and Methylococcus with 89 and 84 % derived amino acid sequence identity to Methylococcus capsulatus Bath and Methylocaldum gracile VKM-14LT, respectively. The DNA G+C content was 63.1 mol% and the major cellular fatty acid was C16 : 0 (62.05 %). Thus, strain E10T ( = JCM 16910T = DSM 23452T) represents the type strain of a novel species within a new genus, for which the name Methylogaea oryzae gen. nov., sp. nov. is proposed.
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Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand
More LessA Gram-staining-negative, motile, agarolytic bacterium, designated M-M1T, was isolated from marine sand obtained from Geoje Island, South Sea, Korea, and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain M-M1T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. It did not grow in the presence of >7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M-M1T fell within the clade comprising members of the genus Thalassomonas, clustering with Thalassomonas agarivorans TMA1T, Thalassomonas loyana CBMAI 722T and Thalassomonas ganghwensis JC2041T, with which it exhibited 16S rRNA gene sequence similarity values of 96.4, 96.0 and 94.9 % respectively. Strain M-M1T exhibited 94.7–95.2 % 16S rRNA gene sequence similarity to the other species of the genus Thalassomonas. Strain M-M1T contained Q-8 as the predominant ubiquinone and C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c as the major fatty acids. The DNA G+C content was 44.2 mol%. Strain M-M1T could be differentiated from phylogenetically related species of the genus Thalassomonas by differences in some phenotypic properties. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain M-M1T is considered to represent a novel species of the genus Thalassomonas, for which the name Thalassomonas agariperforans sp. nov. is proposed. The type strain is M-M1T ( = KCTC 23343T = CCUG 60020T).
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Kaistia geumhonensis sp. nov. and Kaistia dalseonensis sp. nov., two members of the class Alphaproteobacteria
More LessTwo strains, designated B1-1T and B6-8T, were isolated from the Geumho River and the Dalseo Stream in Korea. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class Alphaproteobacteria, their closest relatives being Kaistia adipata KCTC 12095T, Kaistia granuli KCTC 12575T, Kaistia soli KACC 12605T and Kaistia terrae KACC 12910T with 16S rRNA gene sequence similarities of 95.3 –97.7 % to the two novel strains. Strains B1-1T and B6-8T shared a 16S rRNA gene sequence similarity value of 96.1 %. Cells of the two strains were Gram-reaction-negative, aerobic, non-motile, short rods or cocci. The predominant ubiquinone was Q-10. The major fatty acids were C16 : 0, C18 : 1ω7c, C18 : 0 and C19 : 0ω8c cyclo for strain B1-1T and C16 : 0, C18 : 1ω7c, C18 : 0, C18 : 1 2-OH, and C19 : 0ω8c cyclo for strain B6-8T. The G+C contents of the genomic DNA of the strains B1-1T and B6-8T were 61.6 and 66.5 mol%, respectively. Based on the results of this polyphasic study, strains B1-1T ( = KCTC 12849T = DSM 18799T) and B6-8T ( = KCTC 12850T = DSM 18800T) represent two novel species of the genus Kaistia, for which the names Kaistia geumhonensis sp. nov. and Kaistia dalseonensis sp. nov. are proposed, respectively.
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Rhizobium vallis sp. nov., isolated from nodules of three leguminous species
More LessFour bacterial strains isolated from root nodules of Phaseolus vulgaris, Mimosa pudica and Indigofera spicata plants grown in the Yunnan province of China were identified as a lineage within the genus Rhizobium according to the analysis of 16S rRNA gene sequences, sharing most similarity with Rhizobium lusitanum P1-7T (99.1 % sequence similarity) and Rhizobium rhizogenes IAM 13570T (99.0 %). These strains also formed a distinctive group from the reference strains for defined species of the genus Rhizobium in a polyphasic approach, including the phylogenetic analyses of the 16S rRNA gene and housekeeping genes (recA, atpD, glnII), DNA–DNA hybridization, BOX-PCR fingerprinting, phenotypic characterization, SDS-PAGE of whole-cell proteins, and cellular fatty acid profiles. All the data obtained in this study suggested that these strains represent a novel species of the genus Rhizobium, for which the name Rhizobium vallis sp. nov. is proposed. The DNA G+C content (mol%) of this species varied between 60.9 and 61.2 (T m). The type strain of R. vallis sp. nov. is CCBAU 65647T ( = LMG 25295T = HAMBI 3073T), which has a DNA G+C content of 60.9 mol% and forms effective nodules on Phaseolus vulgaris.
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Acidovorax radicis sp. nov., a wheat-root-colonizing bacterium
More LessStrain N35T was isolated from surface-sterilized wheat roots and is a Gram-negative, aerobic, motile straight rod. Strain N35T tested oxidase-positive and catalase-negative and grew optimally at pH 7.0, 30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis showed over 97 % sequence similarity to strains of the environmental species Acidovorax delafieldii, A. facilis, A. defluvii, A. temperans, A. caeni and A. soli, as well as Acidovorax valerianellae, A. anthurii and Simplicispira metamorpha. DNA–DNA hybridization between strain N35T and phylogenetically closely related type strains was 25.3–55.7 %, which clearly separates the strain from these closely related species. Additionally, phenotypic properties, such as substrate metabolism profiles as determined by a Biolog GN2 assay and cell-wall fatty acid profiles, particularly contents of the fatty acids C16 : 0, C16 : 1ω7c/t, C17 : 0, C17 : 0 cyclo, C18 : 0 cyclo and C19 : 0 cyclo, facilitated the differentiation of the newly isolated strain N35T from its closest relatives. The isolate underwent phenotypic variation at high frequency in laboratory media. The DNA G+C content was 64.9 mol%. We propose that strain N35T is classified as a representative of a novel species within the genus Acidovorax, and suggest the name Acidovorax radicis sp. nov. The type strain is strain N35T ( = DSM 23535T = LMG 25767T).
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Citreicella aestuarii sp. nov., isolated from a tidal flat
A Gram-negative bacterium, designated AD8T, was isolated from a tidal flat of the Taean coast in South Korea. Cells were strictly aerobic, motile, ovoid rods or rods, with two lateral flagella on one side. Strain AD8T was catalase- and oxidase-positive and able to oxidize thiosulfate and reduce nitrate to nitrite. Growth of strain AD8T was observed at 15–40 °C (optimum 25–30 °C), at pH 5.0–9.5 (optimum pH 6.0–7.5) and with 1.0–11.0 % (w/v) NaCl (optimum 2.0–4.0 %). The predominant fatty acids were C18 : 1ω7c and C19 : 0 cyclo ω8c. The G+C content of the genomic DNA was 67.5 mol% and the major quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AD8T belonged to the genus Citreicella and was most closely related to Citreicella marina CK-I3-6T within the class Alphaproteobacteria (96.9 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain AD8T represents a novel species within the genus Citreicella, for which the name Citreicella aestuarii sp. nov. is proposed. The type strain is AD8T ( = KACC 13699T = DSM 22011T).
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Halomonas rifensis sp. nov., an exopolysaccharide-producing, halophilic bacterium isolated from a solar saltern
More LessA polyphasic taxonomic study was conducted on strain HK31T, a moderately halophilic bacterium isolated from a solar saltern in Chefchaouen, Morocco. The strain was a Gram-reaction-negative, oxidase-positive rod, which was motile by means of peritrichous flagella. The strain required NaCl for growth and grew in salt concentrations (mixture of sea salts) of 0.5–20 % (w/v) (optimum 5–7.5 %, w/v), at 25–45 °C (optimum 32 °C) and at pH 5–10 (optimum pH 6–9). Strain HK31T did not produce acids from sugars and its metabolism was respiratory, using oxygen as terminal electron acceptor. The strain was positive for the accumulation of poly-β-hydroxyalkanoate granules and formed mucoid colonies due to the excretion of an exopolysaccharide. The DNA G+C content was 61.5 mol%. 16S rRNA gene sequence analysis indicated that it belonged to the genus Halomonas in the class Gammaproteobacteria. The most phylogenetically related species was Halomonas anticariensis, with which strain HK31T showed a 16S rRNA gene sequence similarity of 96.48 %. Its major fatty acids were C18 : 1ω7c, C16 : 0, C19 : 0 cyclo ω8c , C16 : 1ω7c/iso-C15 : 0 2-OH and C12 : 0 3-OH and the predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q-9). Based on the evidence provided in this study, strain HK31T ( = CECT 7698T = LMG 25695T) represents a novel species of the genus Halomonas, for which the name Halomonas rifensis is proposed.
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Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.)
More LessA novel bacterial strain, designated NRCPB10T, was isolated from rhizosphere soil of chickpea (Cicer arietinum L.) in Pusa, New Delhi, India. The 16S rRNA gene sequence of strain NRCPB10T showed highest similarity (98.9 %) to that of Rhizobium radiobacter NCPPB 2437T, followed by Rhizobium larrymoorei AF3-10T (97.7 %) and Rhizobium rubi IFO 13261T (97.4 %). Phylogenetic analysis of strain NRCPB10T based on the housekeeping genes recA and atpD confirmed its position as distinct from recognized Rhizobium species. Levels of DNA–DNA relatedness between strain NRCPB10T and R. radiobacter ICMP 5785T, R. larrymoorei LMG 21410T and R. rubi ICMP 6428T were 51.0, 32.6 and 27.3 %, respectively. Cellular fatty acids of strain NRCPB10T were C18 : 1ω7c (58.9 %), C16 : 0 (15.5 %), C19 : 0 cyclo ω8c (11.5 %), iso-C16 : 1 (5.8 %), C16 : 0 3-OH (4.5 %), C16 : 1ω7c (2.1 %) and C18 : 0 (1.3 %). The G+C content of the genomic DNA of strain NRCPB10T was 59.0 mol%. Strain NRCPB10T did not nodulate chickpea plants or induce tumours in tobacco plants. Phenotypic and physiological properties along with SDS-PAGE of whole-cell soluble proteins differentiated strain NRCPB10T from its closest phylogenetic neighbours. On the basis of data from the present polyphasic taxonomic study, strain NRCPB10T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pusense sp. nov. is proposed. The type strain is NRCPB10T ( = LMG 25623T = JCM 16209T = NCIMB 14639T).
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Methylothermus subterraneus sp. nov., a moderately thermophilic methanotroph isolated from a terrestrial subsurface hot aquifer
A novel methane-oxidizing bacterium, strain HTM55T, was isolated from subsurface hot aquifer water from a Japanese gold mine. Strain HTM55T was a Gram-negative, aerobic, motile, coccoid bacterium with a single polar flagellum and the distinctive intracytoplasmic membrane arrangement of a type I methanotroph. Strain HTM55T was a moderately thermophilic, obligate methanotroph that grew on methane and methanol at 37–65 °C (optimum 55–60 °C). The isolate grew at pH 5.2–7.5 (optimum 5.8–6.3) and with 0–1 % NaCl (optimum 0–0.3 %). The ribulose monophosphate pathway was operative for carbon assimilation. The DNA G+C content was 54.4 mol% and the major fatty acids were C16 : 0 (52.0 %) and C18 : 1ω7c (34.8 %). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain HTM55T was closely related to Methylothermus thermalis MYHTT (99.2 % 16S rRNA gene sequence similarity), which is within the class Gammaproteobacteria. However, DNA–DNA relatedness between strain HTM55T and Methylothermus thermalis MYHTT was ≤39 %. On the basis of distinct phylogenetic, chemotaxonomic and physiological characteristics, strain HTM55T represents a novel species of the genus Methylothermus, for which the name Methylothermus subterraneus sp. nov. is proposed. The type strain is HTM55T ( = JCM 13664T = DSM 19750T).
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Tolumonas osonensis sp. nov., isolated from anoxic freshwater sediment, and emended description of the genus Tolumonas
More LessA polyphasic taxonomic study was performed on a strain of an unknown Gram-negative, non-motile, saccharolytic, facultatively anaerobic bacterium, strain OCF 7T, isolated from anoxic freshwater sediment. The strain grew optimally at 22 °C and pH 7.5, and was able to grow under strictly anaerobic conditions. Major fermentation products from glucose metabolism were formate, acetate, ethanol and lactate. Comparative 16S rRNA gene sequence analysis indicated that strain OCF 7T was phylogenetically related to the type strain of Tolumonas auensis (97.2 % similarity) within the family Aeromonadaceae of the Gammaproteobacteria. However, OCF 7T did not produce toluene from phenylacetate, phenylalanine, phenoxyacetate, phenylsuccinate or phenylbutyrate in the presence of glucose. Phenol was not produced from tyrosine or phenoxyacetate in the presence of glucose. Dominant fatty acids of this micro-organism included C16 : 0, C18 : 1ω7c and C16 : 1ω7c (and/or iso-C15 : 0 2-OH). Major polar lipids were phosphatidylglycerol and phosphatidylethanolamine, and the respiratory quinone was menaquinone MK-8. The genomic DNA G+C content of strain OCF 7T was 52.1 mol%. Based on phylogenetic and phenotypic evidence, strain OCF 7T should be classified as a representative of a novel species of Tolumonas, for which the name Tolumonas osonensis sp. nov. is proposed; the type strain is OCF 7T ( = DSM 22975T = ATCC BAA-1908T). An emended description of the genus Tolumonas is also given.
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Nitratireductor aquimarinus sp. nov., isolated from a culture of the diatom Skeletonema costatum, and emended description of the genus Nitratireductor
More LessTwo Gram-negative-staining, aerobic bacterial strains, designated CL-SC21T and CL-SC22, were isolated from a culture of the diatom Skeletonema costatum (Korean Marine Microalgae Culture Center, KMMCC strain B-396) established from the East Sea, Korea. The two novel strains shared 99.9 % 16S rRNA gene sequence similarity. Analysis of the 16S rRNA gene sequences showed an affiliation with the genus Nitratireductor, with the strains sharing 96.5–97.5 % similarity with the type strains of recognized species of the genus Nitratireductor and being most closely related to Nitratireductor aquibiodomus NL21T. Phylogenetic analyses of the 16S rRNA gene sequences showed that strain CL-SC21T together with strain CL-SC22 belonged to the genus Nitratireductor and formed a robust clade among closely related Nitratireductor species. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, two unidentified aminophospholipids, an unidentified aminolipid, an unidentified phospholipid and five unidentified lipids. Ubiquinone 10 was the major quinone. The major cellular fatty acids of strains CL-SC21T and CL-SC22 were C18 : 1ω7c (70.6–72.3 %) and C19 : 0ω8c cyclo (10.9–11.8 %). The genomic DNA G+C contents of strains CL-SC21T and CL-SC22 were 56.7 and 57.1 mol%, respectively. The level of DNA–DNA relatedness between strains CL-SC21T and CL-SC22 was 86 % (reciprocal 91 %), indicating that the two isolates represented a single species. However, levels of DNA–DNA relatedness between N. aquibiodomus NL21T and strains CL-SC21T and CL-SC22 were 28 % (reciprocal 45 %) and 25 % (reciprocal 50 %), respectively. Phylogenetic analysis and the results of biochemical tests showed that strains CL-SC21T and CL-SC22 were different from all recognized species of the genus Nitratireductor. Thus, strains CL-SC21T and CL-SC22 represent a novel species of the genus Nitratireductor, for which the name Nitratireductor aquimarinus sp. nov. is proposed. The type strain is CL-SC21T ( = KCCM 90090T = JCM 17288T).
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Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant
More LessA Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11T, was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11T belonged to the family Brucellaceae, class Alphaproteobacteria, and was most closely related to Ochrobactrum ciceri Ca-34T (97.9 % sequence similarity) and Ochrobactrum pituitosum CCUG 50899T (96.4 %). Comparative sequence analyses of the additional phylogenetic marker genes dnaK, groEL and gyrB confirmed the affiliation of strain MJ11T to the genus Ochrobactrum. The G+C content of the genomic DNA of strain MJ11T was 59.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with C18 : 1ω7c (62.6 %) and C19 : 0 cyclo ω8c (14.2 %) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11T to the genus Ochrobactrum. The DNA–DNA relatedness between strain MJ11T and Ochrobactrum ciceri DSM 22292T was 29±7 %, clearly showing that the isolate constitutes a new genospecies. Strain MJ11T could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11T represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum daejeonense sp. nov. is proposed. The type strain is MJ11T ( = KCTC 22458T = JCM 16234T).
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Desulfovibrio piezophilus sp. nov., a piezophilic, sulfate-reducing bacterium isolated from wood falls in the Mediterranean Sea
A novel sulfate-reducing bacterium, designated C1TLV30T, was isolated from wood falls at a depth of 1693 m in the Mediterranean Sea. Cells were motile vibrios (2–4×0.5 µm). Strain C1TLV30T grew at temperatures between 15 and 45 °C (optimum 30 °C) and at pH 5.4–8.6 (optimum 7.3). It required NaCl for growth (optimum at 25 g NaCl l−1) and tolerated up to 80 g NaCl l−1. Strain C1TLV30T used as energy sources: lactate, fumarate, formate, malate, pyruvate and ethanol. The end products from lactate oxidation were acetate, H2S and CO2 in the presence of sulfate as terminal electron acceptor. Besides sulfate, thiosulfate and sulfite were also used as terminal electron acceptors, but not elemental sulfur, fumarate, nitrate or nitrite. Strain C1TLV30T possessed desulfoviridin and was piezophilic, growing optimally at 10 MPa (range 0–30 MPa). The membrane lipid composition of this strain was examined to reveal an increase in fatty acid chain lengths at high hydrostatic pressures. The G+C content of the genomic DNA was 49.6 % and the genome size was estimated at 3.5±0.5 Mb. Phylogenetic analysis of the SSU rRNA gene sequence indicated that strain C1TLV30T was affiliated to the genus Desulfovibrio with Desulfovibrio profundus being its closest phylogenetic relative (similarity of 96.4 %). On the basis of SSU rRNA gene sequence comparisons and physiological characteristics, strain C1TLV30T ( = DSM 21447T = JCM 1548T) is proposed to be assigned to a novel species of the genus Desulfovibrio, Desulfovibrio piezophilus sp. nov.
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Reclassification of Aurantimonas altamirensis (Jurado et al. 2006), Aurantimonas ureilytica (Weon et al. 2007) and Aurantimonas frigidaquae (Kim et al. 2008) as members of a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov., Aureimonas ureilytica comb. nov. and Aureimonas frigidaquae comb. nov., and emended descriptions of the genera Aurantimonas and Fulvimarina
More LessMembers of the genera Aurantimonas and Fulvimarina have largely been described on the basis of 16S rRNA gene sequence analyses, biochemical tests and limited chemotaxonomic data. Examination of the polar lipid compositions of members of these two genera indicated that although the patterns were similar with regards the phospholipid and amino lipid compositions, there were clear differences in the presence or absence of the glycolipid sulfoquinovosyldiacylglycerol (SQDG). This glycolipid was absent in members of the species Aurantimonas coralicida and Fulvimarina pelagi but was present in Aurantimonas altamirensis, Aurantimonas ureilytica and Aurantimonas frigidaquae. These results are also consistent with the 16S rRNA sequence-based grouping. We therefore propose that the genus Aurantimonas be divided further to reflect this additional information. Thus, it is recommended that Aurantimonas altamirensis, Aurantimonas ureilytica and Aurantimonas frigidaquae are transferred to a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov. (type strain S21BT = CECT 7138T = CIP 109525T = KCTC 22106T = LMG 23375T = DSM 21988T), Aureimonas ureilytica comb. nov. (type strain 5715S-12T = KACC 11607T = DSM 18598T = CIP 109815T) and Aureimonas frigidaquae comb. nov. (type strain CW5T = KCTC 12893T = JCM 14755T = DSM 21987T). The type species of the new genus is Aureimonas altamirensis. Additionally, emended descriptions of the genera Aurantimonas and Fulvimarina are provided.
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Luteimonas lutimaris sp. nov., isolated from a tidal flat
More LessA Gram-staining-negative, strictly aerobic bacterium, designated strain G3T, was isolated from a tidal flat of the Taean coast in South Korea. Cells were moderately halotolerant and non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain G3T was observed between 15 and 40 °C (optimum 30 °C) and between pH 5.5 and 9.0 (optimum pH 6.5–7.5). Strain G3T contained Q-8 as the predominant lipoquinone and iso-C15 : 0, iso-C17 : 1ω9c, iso-C16 : 0 and iso-C11 : 0 as the major fatty acids. The G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain G3T formed a tight phylogenetic lineage with Luteimonas mephitis B1953/27.1T within the genus Luteimonas and was most closely related to L. mephitis B1953/27.1T with 98.0 % 16S rRNA gene sequence similarity. The DNA–DNA relatedness between strain G3T and L. mephitis B1953/27.1T was 35.2±3.3 %. On the basis of chemotaxonomic data and molecular properties, strain G3T represents a novel species of the genus Luteimonas, for which the name Luteimonas lutimaris sp. nov. is proposed. The type strain is G3T ( = KACC 14929T = JCM 16916T).
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Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi
Three endophytic bacterial isolates were obtained in Italy from olive knots caused by Pseudomonas savastanoi pv. savastanoi. Phenotypic tests in combination with 16S rRNA gene sequence analysis indicated a phylogenetic position for these isolates in the genera Erwinia or Pantoea, and revealed two other strains with highly similar 16S rRNA gene sequences (>99 %), CECT 5262 and CECT 5264, obtained in Spain from olive knots. Rep-PCR DNA fingerprinting of the five strains from olive knots with BOX, ERIC and REP primers revealed three groups of profiles that were highly similar to each other. Multilocus sequence analysis (MLSA) based on concatenated partial atpD, gyrB, infB and rpoB gene sequences indicated that the strains constituted a single novel species in the genus Erwinia. The strains showed general phenotypic characteristics typical of the genus Erwinia and whole genome DNA–DNA hybridization data confirmed that they represented a single novel species of the genus Erwinia. The strains showed DNA G+C contents ranging from 54.7 to 54.9 mol%. They could be discriminated from phylogenetically related species of the genus Erwinia by their ability to utilize potassium gluconate, l-rhamnose and d-arabitol, but not glycerol, inositol or d-sorbitol. The name Erwinia oleae sp. nov. (type strain DAPP-PG 531T = LMG 25322T = DSM 23398T) is proposed for this novel taxon.
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Enterobacter mori sp. nov., associated with bacterial wilt on Morus alba L.
Two isolates of mulberry-pathogenic bacteria isolated from diseased mulberry roots were investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated strains R18-2T and R3-3 to the genus Enterobacter, with Enterobacter asburiae, E. amnigenus, E. cancerogenus, E. cloacae subsp. cloacae, E. cloacae subsp. dissolvens and E. nimipressuralis as their closest relatives. Cells of the isolates were Gram-negative, facultatively anaerobic rods, 0.3–1.0 µm wide and 0.8–2.0 µm long, with peritrichous flagella, showing a DNA G+C content of 55.1±0.5 mol%. Calculation of a similarity index based on phenotypic features and fatty acid methyl ester (FAME) analysis suggested that these isolates are members of E. cancerogenus or E. asburiae or a closely related species. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by the presence of lysine decarboxylase activity and their ability to utilize d-arabitol. DNA–DNA relatedness also distinguished the two isolates from phylogenetically closely related Enterobacter strains. Based on these data, it is proposed that the isolates represent a novel species of the genus Enterobacter, named Enterobacter mori sp. nov. The type strain is R18-2T ( = CGMCC 1.10322T = LMG 25706T).
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- Actinobacteria
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Solirubrobacter ginsenosidimutans sp. nov., isolated from soil of a ginseng field
More LessA Gram-reaction-positive, non-motile, non-spore-forming, aerobic rod, designated BXN5-15T, was isolated from the soil of a ginseng field on Baekdu Mountain in China. Strain BXN5-15T grew optimally at 30 °C and pH 6.0–7.0 in the absence of NaCl on R2A agar. Strain BXN5-15T displayed β-glucosidase activity, which allowed it to transform ginsenoside Rb1 (one of the dominant active components of ginseng) via Rd to minor ginsenoside F2. On the basis of 16S rRNA gene sequence analysis, strain BXN5-15T was shown to belong to the genus Solirubrobacter. The closest phylogenetic relatives were Solirubrobacter soli Gsoil 355T (98.4 % 16S rRNA gene sequence similarity) and Solirubrobacter pauli B33D1T (96.4 %). Lower sequence similarities (<96.0 %) were found with all of the other recognized members of the order Solirubrobacterales. The predominant quinone was MK-7(H4). The major fatty acids (>10 %) were C18 : 1ω9c, iso-C16 : 0 and C18 : 3ω6,9,12c. The G+C content of the genomic DNA was 70.6 mol%. DNA−DNA relatedness between strain BXN5-15T and S. soli KCTC 12628T was 23.3 %. On the basis of genotypic, phenotypic and chemotaxonomic data, strain BXN5-15T represents a novel species within the genus Solirubrobacter, for which the name Solirubrobacter ginsenosidimutans sp. nov. is proposed. The type strain is BXN5-15T ( = KACC 20671T = LMG 24459T).
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Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces
Strains HM2-1 and HM2-2T were isolated from the faeces of a healthy infant and were characterized by determining their phenotypic and biochemical features and phylogenetic positions based on partial 16S rRNA gene sequence analysis. They were Gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, and catalase-negative non-motile rods. They did not grow at 15 or 45 °C in anaerobic bacterial culture medium, and their DNA G+C content was in the range 56–59 mol%. In enzyme activity tests, strains HM2-1 and HM2-2T were positive for α/β-galactosidases and α/β-glucosidases but negative for β-glucuronidase and cystine arylamidase. An analysis of the cell-wall composition of strains HM2-1 and HM2-2T revealed the presence of glutamic acid, alanine and lysine. The presence of fructose-6-phosphate phosphoketolase shows that isolates HM2-1 and HM2-2T are members of the genus Bifidobacterium. These two isolates belong to the same species of the genus Bifidobacterium. Strain HM2-2T was found to be related to Bifidobacterium catenulatum JCM 1194T (97.4 % 16S rRNA gene sequence identity: 1480/1520 bp), Bifidobacterium pseudocatenulatum JCM 1200T (97.2 %: 1472/1514 bp), Bifidobacterium dentium ATCC 27534T (96.7 %: 1459/1509 bp) and Bifidobacterium angulatum ATCC 27535T (96.5 %: 1462/1515 bp). The predominant cellular fatty acids of strains HM2-1 and HM2-2T were 16 : 0 and 18 : 1ω9c, with proportions greater than 18 % of the total. Phylogenetic analyses involving phenotypic characterization, DNA–DNA hybridization and partial 16S rRNA gene sequencing proves that the strains represent a novel species of the genus Bifidobacterium, for which the name Bifidobacterium kashiwanohense sp. nov. is proposed. The type strain is HM2-2T ( = JCM 15439T = DSM 21854T).
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Actinopolymorpha pittospori sp. nov., an endophyte isolated from surface-sterilized leaves of an apricot tree (Pittosporum phylliraeoides)
More LessA member of the genus Actinopolymorpha, designated PIP 143T, was isolated from the leaves of an Australian native apricot tree (Pittosporum phylliraeoides). The isolate was a Gram-reaction-positive, aerobic actinobacterium, with a well-developed substrate mycelium that fragmented into small rods. Phylogenetic evaluation based on 16S rRNA gene sequences placed the isolate in the family Nocardioidaceae. Strain PIP 143T was most closely related to Actinopolymorpha cephalotaxi I06-2230T (98.7 %) and Actinopolymorpha rutila YIM 45725T (98.1 %). Chemotaxonomic data, including cell-wall components, menaquinones and fatty acids, confirmed the affiliation of strain PIP 143T to the genus Actinopolymorpha. Phylogenetic analysis and physiological and biochemical studies, in combination with DNA–DNA hybridization studies, allowed the differentiation of strain PIP 143T from its closest phylogenetic neighbours with validly published names. Therefore, a novel species is proposed, with the name Actinopolymorpha pittospori sp. nov. The type strain is PIP 143T ( = DSM 45354T = ACM 5288T = NRRL B-24810T).
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Actinomycetospora rishiriensis sp. nov., isolated from a lichen
An actinomycete, strain RI109-Li102T, was isolated from a lichen sample obtained from Rishiri Island in Japan. Cells of strain RI109-Li102T were Gram-positive, aerobic and non-motile and formed bud-like spore chains. The isolate grew with 0–3 % (w/v) NaCl, at pH 5–9 and at 10–30 °C (optimum 30 °C). The whole-cell hydrolysate contained meso-diaminopimelic acid, arabinose and galactose. The predominant menaquinone was MK-8(H4) and the diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were iso-C16 : 0 and iso-C16 : 1 H. Comparative 16S rRNA gene sequence analysis revealed that strain RI109-Li102T was most closely related to Actinomycetospora corticicola 014-5T (99.0 % rRNA gene sequence similarity) and Actinomycetospora chiangmaiensis YIM 0006T (98.4 %). However, DNA–DNA hybridization assays, as well as physiological and biochemical analyses, showed that strain RI109-Li102T could be differentiated from its closest phylogenetic relatives. It is proposed that strain RI109-Li102T ( = NBRC 106356T = KCTC 19782T) be classified as the type strain of a novel species, with the name Actinomycetospora rishiriensis sp. nov.
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Nocardiopsis flavescens sp. nov., an actinomycete isolated from marine sediment
More LessA Gram-positive, aerobic bacterium, strain SA6T, was isolated from marine sediment taken at a depth of 20 cm on the seashore of Lianyungang, Jiangsu Province, China. Strain SA6T contained meso-diaminopimelic acid, no diagnostic sugars, type PIII phospholipids, and MK-10(H2) and MK-10(H4) as the predominant menaquinones. The organism showed a range of chemical and morphological properties consistent with its classification in the genus Nocardiopsis. The almost-complete 16S rRNA gene sequence of strain SA6T was aligned with corresponding sequences of representatives of the genus Nocardiopsis and related taxa by using two tree-making algorithms. Strain SA6T formed a distinct phyletic line within the evolutionary radiation occupied by the genus Nocardiopsis and was related most closely to the type strain of Nocardiopsis lucentensis. Strain SA6T could be distinguished from its nearest phylogenetic relatives in the genus Nocardiopsis based on DNA–DNA relatedness data and a combination of phenotypic properties. Strain SA6T should therefore be assigned to the genus Nocardiopsis as a representative of a novel species, for which the name Nocardiopsis flavescens sp. nov. is proposed. The type strain is SA6T ( = CGMCC 4.5723T = JCM 17424T).
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Streptomyces sundarbansensis sp. nov., an actinomycete that produces 2-allyloxyphenol
More LessA novel actinomycete producing 2-allyloxyphenol, designated strain MS1/7T, was isolated from sediments of the Sundarbans mangrove forest, India. Growth on International Streptomyces Project (ISP) media 2, 3, 4, 5 and 7 produced olive green to grey aerial hyphae that carried smooth-surfaced spores in a flexuous (Rectiflexibiles) arrangement. The strain contained ll-diaminopimelic acid, but no diagnostic sugars in whole-cell hydrolysates. Hexa-, octa- and a minor amount of tetra-hydrogenated menaquinones with nine isoprene units [MK-9 (H4, H6, H8 and H10)] were present as isoprene analogues. Diagnostic phospholipids were phosphatidylethanolamine and diphosphatidylglycerol. The predominant fatty acids were anteiso-C15 : 0 (34.80 %), iso-C16 : 0 (16.45 %), C16 (10.53 %) and anteiso-C17 : 0 (10.92 %). The strain showed greater than 99 % 16S rRNA gene sequence similarity to the type strains of several recognized species of the genus Streptomyces, but in the phylogenetic tree based on 16S rRNA gene sequences it formed a distinct phyletic line and demonstrated closest relationships to viomycin-producers (Streptomyces californicus NRRL B-1221T, Streptomyces floridae MTCC 2534T and Streptomyces puniceus NRRL B-2895T). However, strain MS1/7T could be distinguished from these and other closely related species based on low levels of DNA–DNA relatedness (<44 %) and disparate physiological features, principally amino acid utilization and growth in NaCl. Strain MS1/7T is therefore suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces sundarbansensis sp. nov. is proposed. The type strain is MS1/7T ( = MTCC 10621T = DSM 42019T).
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Williamsia phyllosphaerae sp. nov., isolated from the surface of Trifolium repens leaves
More LessA Gram-positive-staining, non-endospore-forming actinobacterium, designated C7T, was isolated from the leaf surface of Trifolium repens. On the basis of 16S rRNA gene sequence analysis, strain C7T was shown to belong to the genus Williamsia and was most closely related to Williamsia maris SJS0289/JS1T (98.0 % 16S rRNA gene sequence similarity), Williamsia deligens IMMIB RIV-956T (96.4 %) and Williamsia serinedens IMMIB SR-4T (95.7 %). The quinone system consisted predominantly of the menaquinones MK-9(H2), MK-8(H2) and MK-7(H2). The major components in the polar lipid profile were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. Mycolic acids were present. These chemotaxonomic traits and the major fatty acids, which were C16 : 1ω7c, C16 : 0, C18 : 0, C18 : 1ω9c and tuberculostearic acid, supported the affiliation of strain C7T with the genus Williamsia. Physiological and biochemical analysis revealed clear differences between strain C7T and its closest phylogenetic neighbours. Therefore, strain C7T represents a novel species, for which the name Williamsia phyllosphaerae sp. nov. is proposed. The type strain is C7T ( = CCUG 60465T = CCM 7855T).
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Streptomyces indicus sp. nov., an actinomycete isolated from deep-sea sediment
Yingxue Luo, Jing Xiao, Yin Wang, Jing Xu, Shujie Xie and Jun XuThe taxonomic position of an actinomycete isolated from deep-sea sediment from the Indian Ocean was determined by using a polyphasic approach. The presence of iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0 as the major cellular fatty acids, ll-diaminopimelic acid as the characteristic diamino acid, and MK-9(H4, H6 and H8) as the major menaquinones supported the affiliation of strain IH32-1T to the genus Streptomyces. Comparison of 16S rRNA gene sequences showed that strain IH32-1T exhibited highest similarities to the type strains of Streptomyces globosus (97.6 %) and Streptomyces toxytricini (97.6 %). However, DNA–DNA relatedness values between strain IH32-1T and the type strains of S. globosus and S. toxytricini were determined as 55.2±4.7 and 38.3±2.5 %, respectively. Based on its chemotaxonomic, phenotypic and genotypic characteristics, strain IH32-1T is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces indicus sp. nov. is proposed. The type strain is IH32-1T ( = DSM 42001T = CGMCC 4.5727T).
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Nonomuraea maritima sp. nov., isolated from coastal sediment
More LessA novel actinomycete, strain FXJ7.203T, was isolated from a coastal sediment sample collected in Bohai Bay, China. In 16S rRNA gene sequence analyses, strain FXJ7.203T always formed a unique monophyletic line within the genus Nonomuraea and was most closely related to Nonomuraea turkmeniaca (97.9 %), Nonomuraea candida (97.8 %), Nonomuraea helvata (97.7 %) and Nonomuraea rubra (97.5 %). Morphological and chemotaxonomic characteristics supported the allocation of the strain to the genus Nonomuraea. The polar lipid profile showed that its phospholipid type was PIV. The major fatty acids were iso-C16 : 0, C17 : 1ω6c, iso-C16 : 1 G, 10-methyl C17 : 0 and iso-C15 : 0. The major menaquinone was MK-9(H4) with minor amounts of MK-9(H2), MK-9(H0) and MK-9(H6). The results of physiological and biochemical tests allowed differentiation of strain FXJ7.203T from closely related species. Based on the genotypic and phenotypic data, strain FXJ7.203T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea maritima sp. nov. is proposed, with strain FXJ7.203T ( = CGMCC 4.5681T = NBRC 106687T) as the type strain.
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- Firmicutes and Related Organisms
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Clostridium chromiireducens sp. nov., isolated from Cr(VI)-contaminated soil
More LessA Cr(VI)-resistant, Gram-positive, spore-forming, obligate anaerobe, designated GCAF-1T, was isolated from chromium-contaminated soil by its ability to reduce Cr(VI) in low concentrations. Mixed acid fermentation during growth on glucose resulted in accumulation of acetate, butyrate, formate and lactate. Morphological studies indicated the presence of peritrichous flagella, pili and an S-layer. The major cellular fatty acids (>5 %) were C16 : 0, C14 : 0, summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c), C18 : 1ω7c, C16 : 1ω9c, summed feature 4 (comprising iso-C17 : 1 I and/or anteiso-C17 : 1 B) and C18 : 1ω9c. The DNA G+C content of strain GCAF-1T was 30.7 mol%. Phylogenetic interference indicated that strain GCAF-1T clustered with group I of the genus Clostridium. Of strains within this cluster, strain GCAF-1T shared the highest 16S rRNA gene sequence similarities (98.1–98.9 %) with Clostridium beijerinckii DSM 791T, C. saccharobutylicum NCP 262T, C. saccharoperbutylacetonicum N1-4T, C. puniceum DSM 2619T and C. roseum DSM 51T. However, strain GCAF-1T could be clearly distinguished from its closest phylogenetic neighbours by low levels of DNA–DNA relatedness (<50 %) and some phenotypic features. Based on the evidence presented here, strain GCAF-1T ( = DSM 23318T = KCTC 5935T) represents a novel species of the genus Clostridium, for which the name Clostridium chromiireducens sp. nov. is proposed.
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Paenibacillus sacheonensis sp. nov., a xylanolytic and cellulolytic bacterium isolated from tidal flat sediment
A Gram-negative-staining, rod-shaped bacterium, designated strain SY01T, was isolated from tidal flat sediment from Sacheon Bay, South Korea. Strain SY01T was characterized with respect to its phenotypic and phylogenetic characteristics. The novel strain was spore-forming, motile, catalase-negative and oxidase-positive. Optimal growth of the strain occurred at 30 °C and pH 7.0. The DNA G+C content was 56.1 mol%. The predominant menaquinone was MK-7. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown lipids were detected in the polar lipid profile. Anteiso-C15 : 0 (47.2 %), iso-C15 : 0 (18.9 %) and iso-C16 : 0 (10.5 %) were the major cellular fatty acids of strain SY01T. The highest 16S rRNA gene sequence similarities were found with Paenibacillus phyllosphaerae PALXIL04T (95.9 %), Paenibacillus tarimensis SA-7-6T (94.6 %) and Paenibacillus mendelii C/2T (94.4 %). Based on the phylogenetic, chemotaxonomic and physiological characteristics presented in this study, strain SY01T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus sacheonensis sp. nov. is proposed. The type strain is SY01T ( = DSM 23054T = KACC 14895T).
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Weissella ceti sp. nov., isolated from beaked whales (Mesoplodon bidens)
During an investigation into the microbiota of beaked whales (Mesoplodon bidens), nine isolates were obtained from different organs of four animals. The isolates were Gram-positive-staining, catalase-negative, short rod-shaped or coccoid organisms. A phylogenetic analysis based on 16S rRNA gene sequences of these isolates allocated them to the genus Weissella, showing 96.3 % and 96.0 % 16S rRNA gene sequence similarity with Weissella viridescens NRIC 1536Tand Weissella minor NRIC 1625T, respectively. On the basis of phenotypic, physiological and phylogenetic evidence, it is proposed that the new isolates from whales represent a novel species of the genus Weissella, Weissella ceti sp. nov. The type strain of Weissella ceti is 1119-1A-09T ( = CECT 7719T = CCUG 59653T).
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Paenibacillus wooponensis sp. nov., isolated from wetland freshwater
More LessA rod-shaped, endospore-forming, Gram-reaction-positive bacterium, designated strain WPCB018T, was isolated from a fresh water sample collected from Woopo wetland, Korea. The isolate was identified as a member of the genus Paenibacillus on the basis of phenotypic characteristics and phylogenetic inference based on 16S rRNA gene sequence analysis. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and unknown aminophospholipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C15 : 0 (32.2 %), C16 : 0 (20.1 %) and C18 : 0 (18.1 %). The DNA G+C content was 56.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WPCB018T belongs to a cluster comprising species of the genus Paenibacillus, its closest neighbours being Paenibacillus humicus PC-147T (97.5 %) and Paenibcillus pasadenensis SAFN-007T (96.2 %). Genomic DNA–DNA hybridizations performed with strain WPCB018T and type strains of the species P. humicus, P. pinihumi, P. phyllosphaerae, P. pasadenensis and P. tarimensis showed relatedness values of only 10, 17, 18, 19 and 20 %, respectively. On the basis of phenotypic, molecular and genetic evidence, strain WPCB018T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus wooponensis sp. nov. is proposed. The type strain of the novel species is WPCB018T ( = KCTC 13280T = JCM 16350T).
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- Bacteroidetes
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Gramella gaetbulicola sp. nov., a member of the family Flavobacteriaceae isolated from foreshore soil
A Gram-reaction-negative, yellow-pigmented, gliding, rod-shaped, aerobic bacterium (RA5-111T) was isolated from foreshore soil. The taxonomic status of the novel isolate was determined using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain RA5-111T could be assigned to the genus Gramella, with sequence similarities of 97.7, 97.3 and 96.2 % to the type strains of Gramella echinicola, Gramella portivictoriae and Gramella marina, respectively. Chemotaxonomic and phenotypic characteristics also supported the affiliation of strain RA5-111T with the genus Gramella. The genomic DNA G+C content was 39.1 mol%. The isolate contained MK-6 as the predominant menaquinone, iso-C15 : 0, iso-C17 : 0 3-OH and a summed feature (iso-C15 : 0 2-OH and/or C16 : 1ω7c) as major fatty acids, and phosphatidylethanolamine and unknown phospholipids as the polar lipids. DNA–DNA relatedness, phenotypic, genotypic and chemotaxonomic data clearly indicate that the isolate represents a novel species of the genus Gramella, for which the name Gramella gaetbulicola sp. nov. is proposed. The type strain is RA5-111T ( = KCTC 23022T = JCM 16528T = NBRC 106272T).
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Elizabethkingia anophelis sp. nov., isolated from the midgut of the mosquito Anopheles gambiae
More LessThe taxonomic position, growth characteristics and antibiotic resistance properties of a slightly yellow-pigmented bacterial strain, designated R26T, isolated from the midgut of the mosquito Anopheles gambiae, were studied. The isolate produced rod-shaped cells, which stained Gram-negative. The bacterium had two growth optima at 30–31 °C and 37 °C. Strain R26T demonstrated natural antibiotic resistance to ampicillin, chloramphenicol, kanamycin, streptomycin and tetracycline. 16S rRNA gene sequence analysis revealed that the isolate showed 98.6 % sequence similarity to that of Elizabethkingia meningoseptica ATCC 13253T and 98.2 % similarity to that of Elizabethkingia miricola GTC 862T. The major fatty acids of strain R26T were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c/t). Strain R26T contained only menaquinone MK-6 and showed a complex polar lipid profile consisting of diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and unknown polar lipids and glycolipids. DNA–DNA hybridization experiments with E. meningoseptica CCUG 214T ( = ATCC 13253T) and E. miricola KCTC 12492T ( = GTC 862T) gave relatedness values of 34.5 % (reciprocal 41.5 %) and 35.0 % (reciprocal 25.7 %), respectively. DNA–DNA hybridization results and some differentiating biochemical properties indicate that strain R26T represents a novel species, for which the name Elizabethkingia anophelis sp. nov. is proposed. The type strain is R26T ( = CCUG 60038T = CCM 7804T).
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Flavobacterium phragmitis sp. nov., an endophyte of reed (Phragmites australis)
A Gram-staining-negative bacterium, designated strain BLN2T, was isolated from within the roots of reeds (Phragmites australis) in Beijing Cuihu Wetland (China) and characterized using a polyphasic taxonomic approach. The cells were yellow-pigmented, rod-shaped, strictly aerobic and devoid of flagella, but showed gliding motility. Strain BLN2T produced yellow, translucent, circular and convex colonies, with optimal growth at 30 °C and pH 7.0. The major respiratory quinone was menaquinone 6 (MK-6) and the predominant fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 3-OH, C16 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH. The G+C content of the genomic DNA was 34.8 mol%. The 16S rRNA gene sequence analysis showed that strain BLN2T belonged to the genus Flavobacterium and was most closely related to Flavobacterium anhuiense CGMCC 1.6859T (97.0 % sequence similarity). The DNA–DNA relatedness between strain BLN2T and F. anhuiense CGMCC 1.6859T was 25.7 %. Based on the phenotypic data and phylogenetic inference presented, it is concluded that strain BLN2T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium phragmitis sp. nov. is proposed. The type strain is BLN2T ( = DSM 23314T = CGMCC 1.10370T).
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Flavobacterium chungbukense sp. nov., isolated from soil
More LessA yellow-pigmented, Gram-staining-negative, non-motile, strictly aerobic and rod-shaped bacterium, designated CS100T, was isolated from soil in Chungbuk, Korea. Phylogenetic analysis and comparative studies based on the 16S rRNA gene sequence showed that strain CS100T belonged to the genus Flavobacterium in the family Flavobacteriaceae. Strain CS100T showed the highest sequence similarities to Flavobacterium glaciei JCM 13953T (97.6 %) and Flavobacterium johnsoniae KACC 11410T (97.1 %). Sequence similarity to other members of the genus Flavobacterium was 91.5–97.0 %. Growth occurred at 4–30 °C, at pH 5.0–9.0 and in the presence of 0–2 % (w/v) NaCl. Flexirubin-type pigments were produced. Menaquinone-6 (MK-6) was the major respiratory quinone and the major fatty acids were iso-C15 : 0 (17.3 %), summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c, 15.5 %) and C16 : 0 (11.8 %). The DNA G+C content was 36.4 mol%. Strain CS100T hydrolysed skimmed milk and gelatin, but not chitin or pectin, and showed oxidase and catalase activities. DNA–DNA relatedness was 3.0 % with F. glaciei JCM 13953T and 11.5 % with F. johnsoniae KACC 11410T. On the basis of the evidence from this study, strain CS100T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium chungbukense sp. nov. is proposed. The type strain is CS100T ( = KACC 15048T = JCM 17386T).
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- Archaea
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Halobellus clavatus gen. nov., sp. nov. and Halorientalis regularis gen. nov., sp. nov., two new members of the family Halobacteriaceae
More LessFour halophilic archaeal strains, designated TNN18T, TBN12, TNN28T and TBN19, were isolated from brines sampled from two artificial marine solar salterns in eastern China. Strains TNN18T and TNN28T were isolated from the Tainan marine solar saltern, whereas strains TBN12 and TBN19 were from the Taibei marine solar saltern. Colonies of the four strains were red-pigmented and their cells were pleomorphic, motile, Gram-reaction-negative rods. Strains TNN18T and TBN12 were able to grow at 25–50 °C (optimum 37 °C), in 10–30 % (w/v) NaCl (optimum 15 %), with 0–1.0 M MgCl2 (optimum 0.05 M) and at pH 5.5–9.0 (optimum pH 7.0–7.5), while strains TNN28T and TBN19 were able to grow at 20–50 °C (optimum 37 °C), in 15-30 % (w/v) NaCl (optimum 18–20 %), in 0.005–1.0 M MgCl2 (optimum 0.01–0.3 M) and at pH 6.0–9.0 (optimum pH 7.0–7.5). Cells of these strains lyse in distilled water; minimal NaCl concentrations to prevent cell-lysis are 10 % (w/v) for strains TNN18T and TBN12 and 12 % (w/v) for strains TNN28T and TBN19. The major polar lipids of strains TNN18T and TBN12 were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and one major glycolipid (GL1), which was chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). Minor amounts of other lipids (GL0, GL2, GL3 and GL4) were also detectable. The polar lipid profiles of strains TNN28T and TBN19 contained PG, PGP-Me, GL1, which was chromatographically identical to S-DGD-1, and three to four minor unidentified glycolipids (GL2–GL5). Phylogenetic analyses revealed that strains TNN18T and TBN12 formed a distinct clade with strains of the closest related species, Haloquadratum walsbyi (91.5–91.8 % 16S rRNA gene sequence similarity) and strains TNN28T and TBN19 formed a distinct clade with strains of the species Halosimplex carlsbadense (89.9–93.3 % similarity) and two members of the genus Halorhabdus (92.5–93.3 % similarity). The DNA G+C contents of strains TNN18T, TBN12, TNN28T and TBN19 were 61.5, 62.4, 61.9 and 61.5 mol%, respectively. DNA–DNA hybridization values between strains TNN18T and TBN12, and strains TNN28T and TBN19 were 82.9 % and 88.2 %, respectively. The phenotypic, chemotaxonomic and phylogenetic properties suggest that the four strains represent two novel species of two new genera within the family Halobacteriaceae, for which the names Halobellus clavatus gen. nov., sp. nov. (type strain TNN18T = CGMCC 1.10118T = JCM 16424T) and Halorientalis regularis gen. nov., sp. nov. (type strain TNN28T = CGMCC 1.10123T = JCM 16425T) are proposed.
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- Other Bacteria
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Fervidobacterium riparium sp. nov., a thermophilic anaerobic cellulolytic bacterium isolated from a hot spring
A novel obligately anaerobic, extremely thermophilic, organotrophic bacterium, strain 1445tT, was isolated from a hot spring on Kunashir Island (Kuril Islands, Russia). Cells were motile rods (0.4–0.5×1.0–3.0 µm). The temperature range for growth at pH 7.8 was 46–80 °C, with optimum growth at 65 °C. The pH range for growth at 65 °C was pH 5.7–9.0, with optimum growth at pH 7.8. Growth was not observed at or below 40 °C, at or above 84 °C, at or below pH 5.4 or at or above pH 9.5. The isolate degraded a wide range of substrates including starch, cellulose and cellulose derivatives. Elemental sulfur stimulated growth, but sodium sulfate, sulfite and thiosulfate did not. DNA G+C content was 31 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed that strain 1445tT belonged to the genus Fervidobacterium. 16S rRNA gene sequence similarities with strains of other species of the genus Fervidobacterium were 94.9–98.3 %; the type strain of Fervidobacterium gondwanense was the closest relative of strain 1445tT. DNA–DNA hybridization of strain 1445tT and F. gondwanense AB39T revealed a relatedness value of 20 %. Based on phylogenetic data and physiological properties of the isolate, a novel species, designated Fervidobacterium riparium sp. nov., is proposed with strain 1445tT ( = DSM 21630T = VKM B-2549T) as the type strain.
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- International Committee On Systematics Of Prokaryotes
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- Minutes
Volumes and issues
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Volume 74 (2024)
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Volume 73 (2023)
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Volume 72 (2022 - 2023)
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Volume 71 (2020 - 2021)
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Volume 70 (2020)
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Volume 69 (2019)
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Volume 68 (2018)
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Volume 67 (2017)
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Volume 66 (2016)
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Volume 65 (2015)
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Volume 64 (2014)
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Volume 63 (2013)
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Volume 62 (2012)
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Volume 61 (2011)
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Volume 60 (2010)
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Volume 59 (2009)
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Volume 58 (2008)
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Volume 57 (2007)
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Volume 56 (2006)
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Volume 55 (2005)
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Volume 54 (2004)
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Volume 53 (2003)
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Volume 52 (2002)
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Volume 51 (2001)
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Volume 50 (2000)
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Volume 49 (1999)
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Volume 48 (1998)
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Volume 47 (1997)
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Volume 46 (1996)
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Volume 45 (1995)
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Volume 44 (1994)
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Volume 43 (1993)
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Volume 42 (1992)
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Volume 41 (1991)
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Volume 40 (1990)
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Volume 39 (1989)
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Volume 38 (1988)
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Volume 37 (1987)
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Volume 36 (1986)
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Volume 35 (1985)
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Volume 34 (1984)
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Volume 33 (1983)
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Volume 32 (1982)
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Volume 31 (1981)
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Volume 30 (1980)
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Volume 29 (1979)
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Volume 28 (1978)
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Volume 27 (1977)
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Volume 26 (1976)
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Volume 25 (1975)
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Volume 24 (1974)
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Volume 23 (1973)
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Volume 22 (1972)
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Volume 21 (1971)
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Volume 20 (1970)
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Volume 19 (1969)
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Volume 18 (1968)
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Volume 17 (1967)
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Volume 16 (1966)
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Volume 15 (1965)
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Volume 14 (1964)
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Volume 13 (1963)
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Volume 12 (1962)
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Volume 11 (1961)
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Volume 10 (1960)
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Volume 9 (1959)
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Volume 8 (1958)
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Volume 7 (1957)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)