- Volume 63, Issue Pt_8, 2013
Volume 63, Issue Pt_8, 2013
- Notification List
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Notification that new names and new combinations have appeared in volume 63, part 5, of the IJSEM
This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed according to the rules of priority (i.e. page number and order of valid publication of names in the original articles). Taxonomic opinions included in this list (i.e. the creation of synonyms or the emendation of circumscriptions) cannot be considered as validly published nor, in any other way, approved by the International Committee on Systematics of Prokaryotes and its Judicial Commission.
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- New Taxa
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- Actinobacteria
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Streptomyces chilikensis sp. nov., a halophilic streptomycete isolated from brackish water sediment
More LessA novel actinobacterial strain, designated RC 1830T, was isolated from the sediment of estuarine coastal brackish water lagoon of Chilika Lake, in Khurdha district of Odisha, India, and characterized using a polyphasic approach. Strain RC 1830T was halophilic and alkali-tolerant and found to hydrolyse chitin, starch, tributyrin, lecithin, Tween 80, cellulose, gelatin and casein. The diagnostic presence of ll-diaminopimelic acid, iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, C16 : 0, iso-C17 : 0, anteiso-C17 : 0 as major cellular fatty acids and MK-9(H4 and H6) as major menaquinones noticeably associated the strain to the genus Streptomyces . After comparison and analysis of the near complete 16S rRNA gene sequence with representative strains of other streptomycetes, it was evident that strain RC 1830T belonged to the genus Streptomyces , and exhibited the highest sequence similarities of 99.53 %, 99.25 %, 99.11 %, 99.10 % and 99. 06 % to Streptomyces fragilis DSM 40044T, Streptomyces coelicoflavus NBRC 15399T, Streptomyces flaveolus NBRC 3715T, Streptomyces lavenduligrisesus NBRC 13405T and Streptomyces eurythermus ATCC 14975T, respectively. Reconstruction of a phylogenetic tree for the genus Streptomyces revealed that strain RC 1830T formed a distinct phyletic line and clustered with its most closely related neighbour S. fragilis DSM 40044T. The DNA–DNA relatedness values between strain RC 1830T and the most closely related type strain S. fragilis DSM 40044T were determined to be 17.7±4.55 %. Additionally, morphological, biochemical and physiological tests were able to distinguish the strain from the most closely related type strain S. fragilis DSM 40044T and other closely related neighbours, S. coelicoflavus DSM 41471T and Streptomyces flaveolus DSM 40061T. Based on a range of phenotypic and genotypic properties, strain RC 1830T is suggested to represent a novel species of the genus Streptomyces for which the name Streptomyces chilikensis sp. nov. is proposed. The type strain is RC 1830T ( = JCM 18411T = DSM 42072T).
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Microbacterium saccharophilum sp. nov., isolated from a sucrose-refining factory
A Gram-stain-positive, non-motile, non-spore-forming, rod-shaped bacterium, designated strain K-1T, was isolated from soil at a sucrose refinery in Japan. The strain grew at 9–37 °C (optimum, 30 °C) and at pH 6–11 (optimum, pH 7.0). Phylogenetic analysis based on the full-length 16S rRNA gene sequence of strain K-1T revealed that it was a member of the genus Microbacterium . High 16S rRNA gene sequence similarities were found between strains K-1T and both Microbacterium pumilum NBRC 101279T (99.7 %) and Microbacterium deminutum NRRL B-24453T (99.5 %). However, the DNA–DNA hybridization values between strain K-1T and M. pumilum NBRC 101279T and M. deminutum NRRL B-24453T were only 12 % and 10 %, respectively. The DNA G+C content of strain K-1T was 73 mol%. The major fatty acids of strain K-1T were anteiso-C15 : 0 and anteiso-C17 : 0, and the major menaquinones were MK-12 and MK-13. The diamino acid in the cell-wall peptidoglycan was lysine. On the basis of these results, strain K-1T is considered to represent a novel species of the genus Microbacterium , for which the name Microbacterium saccharophilum sp. nov. is proposed. The type strain is K-1T ( = NBRC 108778T = NCIMB 14782T).
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Streptomyces halophytocola sp. nov., an endophytic actinomycete isolated from the surface-sterilized stems of a coastal halophyte Tamarix chinensis Lour.
A novel actinomycete, designated KLBMP 1284T, was isolated from the surface-sterilized stems of a coastal halophyte Tamarix chinensis Lour. collected from the city of Nantong, Jiangsu Province, east China. The strain was found to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces . Analysis of the 16S rRNA gene sequence of strain KLBMP 1284T revealed that the strain formed a distinct clade within the phylogenetic tree based on 16S rRNA gene sequences and the highest sequence similarity (99.43 %) was to Streptomyces sulphureus NRRL B-1627T. 16S rRNA gene sequence similarity to other species of the genus Streptomyces was lower than 97 %. Based on DNA–DNA hybridization values and comparison of morphological and phenotypic data, KLBMP 1284T could be distinguished from the closest phylogenetically related species, Streptomyces sulphureus NRRL B-1627T. Thus, based on these data, it is evident that strain KLBMP 1284T represents a novel species of the genus Streptomyces , for which the name Streptomyces halophytocola sp. nov. is proposed. The type strain is KLBMP 1284T ( = KCTC 19890T = NBRC 108770T).
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Longimycelium tulufanense gen. nov., sp. nov., a filamentous actinomycete of the family Pseudonocardiaceae
More LessA novel filamentous actinomycete strain, designated TRM 46004T, was isolated from sediment of Aiding Lake in Tulufan Basin (42° 64′ N 89° 26′ E), north-west China. The isolate was characterized using a polyphasic approach. The isolate formed abundant aerial mycelium with few branches and vegetative mycelium, occasionally twisted and coiled; spherical sporangia containing one to several spherical spores developed at the ends of short sporangiophores on aerial mycelium. The G+C content of the DNA was 65.2 mol%. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and xylose, galactose and ribose as the major whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H10). The major fatty acids were iso-C16 : 0 and anteiso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 46004T formed a distinct lineage within the family Pseudonocardiaceae and showed 91.7–96.1 % 16S rRNA gene sequence similarity with members of the family Pseudonocardiaceae . On the basis of the evidence from this polyphasic study, a novel genus and species, Longimycelium tulufanense gen. nov., sp. nov., are proposed. The type strain of Longimycelium tulufanense is TRM 46004T ( = CGMCC 4.5737T = NBRC 107726T).
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Cryobacterium levicorallinum sp. nov., a psychrophilic bacterium isolated from glacier ice
More LessIn this study, two psychrophilic bacterial strains were isolated from the China No. 1 glacier in Xinjiang, north-west China. Cells were Gram-positive rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Cryobacterium . Phylogenetic analysis showed that they clustered together and are most closely related to Cryobacterium luteum CGMCC 1.11210T, Cryobacterium flavum CGMCC 1.11215T, Cryobacterium psychrophilum CGMCC 1.4292T, Cryobacterium psychrotolerans CGMCC 1.5382T and Cryobacterium roopkundense CGMCC 1.10672T. The major cellular fatty acids of the novel strains were anteiso-C15 : 0, anteiso-C15 : 1 A, iso-C16 : 0 and iso-C15 : 0. Both strains contained diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid in the cell membrane. The results of DNA–DNA hybridization and physiological tests allowed the genotypic and phenotypic differentiation of strains Hh34T and Hh28 from related species. However, their high DNA–DNA relatedness showed that they belong to the same novel species. Strain Hh34T ( = NBRC 107883T = CGMCC 1.11211T) was selected as the type strain to represent this novel species, for which the name Cryobacterium levicorallinum sp. nov. is proposed.
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Methylobacterium tarhaniae sp. nov., isolated from arid soil
More LessA reddish-orange-pigmented, Gram-stain-negative, aerobic, facultatively methylotrophic strain, N4211T, isolated from arid soil, collected from Abuja, Nigeria, was analysed by using a polyphasic approach. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain N4211T belonged to the genus Methylobacterium . Strain N4211T was most closely related to Methylobacterium aquaticum GR16T (98.56 %), Methylobacterium platani PMB02T (97.95 %) and Methylobacterium variabile GR3T (97.2 %), and the phylogenetic similarities to all other species of the genus Methylobacterium with validly published names were less than 97.0 %. The major ubiquinones detected were Q-10. The major fatty acids were summed feature 7 (C18 : 1 cis11/t9/t6). The DNA G+C content was 67.3 mol%. DNA–DNA relatedness of strain N4211T and the most closely related strains M. aquaticum DSM 16371T and M. platani KCTC 12901T were 60.0 and 48.2 %, respectively. On the basis of phenotypic, phylogenetic and DNA–DNA hybridization data, strain N4211T is assigned to a novel species of the genus Methylobacterium for which the name Methylobacterium tarhaniae sp. nov. is proposed. The type strain is N4211T( = KCTC 23615T = DSM 25844T).
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Streptomyces wuyuanensis sp. nov., an actinomycete from soil
More LessA novel actinomycete, strain FX61T, was isolated from a saline sample collected from the Inner Mongolian Autonomous Region in China and subjected to a taxonomic study using a polyphasic approach. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The major fatty acids were iso-C16 : 0, anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 1 H, C16 : 0, iso-C14 : 0 and anteiso-C17 : 0. The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides and an unidentified phospholipid. The G+C content of the genomic DNA was 72 mol%. The 16S rRNA gene sequence of the isolate had greater than 98 % similarity with those of Streptomyces griseoincarnatus ATCC 23623T (98.2 %), Streptomyces labedae DSM 41446T (98.2 %), Streptomyces variabilis ATCC 19815T (98.2 %), Streptomyces erythrogriseus ATCC 27427T (98.2 %), Streptomyces matensis ATCC 23935T (98.2 %), Streptomyces althioticus ATCC 19724T (98.2 %) and Streptomyces luteosporeus ATCC 33049T (98.0 %), showing that the novel strain should be assigned to the genus Streptomyces . DNA–DNA hybridizations with the seven above-mentioned members of the genus Streptomyces showed 29.8, 28.5, 27.0, 25.5, 25.0, 23.5 and 22.0 % relatedness, respectively. On the basis of the phenotypic characteristics and genotypic distinctiveness, strain FX61T should be classified as a novel species of the genus Streptomyces , for which the name Streptomyces wuyuanensis sp. nov. is proposed. The type strain is FX61T ( = CGMCC 4.7042T = KCTC 29112T).
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Aquihabitans daechungensis gen. nov., sp. nov., an actinobacterium isolated from reservoir water
More LessA novel Gram-reaction-positive bacterium, strain CH22-21T, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Cells of strain CH22-21T were non-motile, ciliated short rods that formed creamy-white colonies on half-strength modified R2A agar. Chemotaxonomic results showed menaquinone MK-9(H6) as the predominant respiratory menaquinone, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside as major polar lipids, 16 : 1ω5c, 16 : 0, 17 : 1ω8c and 18 : 1ω9c as major fatty acids, and a DNA G+C content of 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order Acidimicrobiales , showing similarity values of <92.3 % with its closest phylogenetic neighbours. The combined genotypic and phenotypic data showed that strain CH22-21T could be distinguished from all genera within the order Acidimicrobiales and represented a novel species of a new genus in the family Iamiaceae , for which the name Aquihabitans dachungensis gen. nov., sp. nov. is proposed. The type strain of Aquihabitans dachungensis is CH22-21T ( = KCTC 19849T = JCM 17787T).
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Actinopolyspora lacussalsi sp. nov., an extremely halophilic actinomycete isolated from a salt lake
More LessA novel halophilic, filamentous actinobacterium, designated strain TRM 40139T, was isolated from a hypersaline habitat in Xinjiang Province, north-west China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-separated sub-branch within the radiation of the genus Actinopolyspora and the organism was related most closely to the type strains of Actinopolyspora alba (97.6 % similarity), Actinopolyspora xinjiangensis (97.6 %) and Actinopolyspora erythraea (97.1 %). However, it had relatively lower mean DNA–DNA relatedness values with the above strains (36.4, 31.3 and 26.1 %, respectively). Optimal growth occurred at 35 °C, at pH 7.0 and in the presence of 12 % (w/v) NaCl. The whole-cell sugar pattern consisted of xylose, glucose, ribose and arabinose. The major fatty acids were iso-C16 : 0 (28.0 %) and anteiso-C17 : 0 (27.6 %). The diagnostic phospholipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown phospholipids. The predominant menaquinones were MK-9(H4) (49.8 %) and MK-10(H4) (24.2 %). The G+C content of the genomic DNA was 66.4 mol%. Strain TRM 40139T therefore represents a novel species of the genus Actinopolyspora , for which the name Actinopolyspora lacussalsi sp. nov. is proposed. The type strain is TRM 40139T ( = KCTC 19657T = CCTCC AA 2012020T).
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Cellulomonas marina sp. nov., isolated from deep-sea water
More LessA bacterial strain FXJ8.089T was isolated from deep-sea water collected from the southwest Indian Ocean (49° 39′ E 37° 47′ S) at a depth of 2800 m, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FXJ8.089T belonged to the genus Cellulomonas and had the highest similarities with Cellulomonas oligotrophica (96.9 %) and Cellulomonas aerilata (96.6 %). It contained MK-9(H4) as the predominant menaquinone. The polar lipids were diphosphatidylglycerol and phosphatidylinositol mannosides. The cell-wall peptidoglycan type was A4β with an interpeptide bridge l-Orn–d-Glu. The cell-wall sugars were glucose, mannose and ribose. The DNA G+C content was 70.3 mol%. The strain also showed a number of physiological and biochemical characteristics that were distinct from the closely related species. Based on phenotypic and genotypic data, strain FXJ8.089T ( = CGMCC 4.6945T = DSM 24960T) represents a novel species of the genus Cellulomonas , for which the name Cellulomonas marina sp. nov. is proposed.
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Actinoplanes siamensis sp. nov., isolated from soil
A Gram-positive filamentous bacterial strain that developed large campanulate sporangia at the ends of sporangiophores on substrate mycelium was isolated from bamboo forest soil in Thailand. According to the results of a polyphasic taxonomic study, our isolate had typical characteristics of members of the genus Actinoplanes . The 16S rRNA gene sequence analysis also indicated that strain A-T 6646T belonged to the genus Actinoplanes , being most closely related to Actinoplanes liguriensis DSM 43865T (97.61 %) and Actinoplanes octamycinicus NBRC 14524T (97.52 %). The DNA–DNA relatedness values, which differentiate the new strain from the most closely related species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained xylose and arabinose. The predominant menaquinone was MK-9(H4). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and anteiso-C15 : 0. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, the isolate is proposed to represent a novel species to be named Actinoplanes siamensis sp. nov. The type strain is A-T 6646T ( = BCC 46194T = NBRC 109076T).
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- Archaea
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Thermococcus prieurii sp. nov., a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent
More LessA novel hyperthermophilic, anaerobic archaeon, strain Bio-pl-0405IT2T, was isolated from a hydrothermal chimney sample collected from the East Pacific Rise at 2700 m depth in the ‘Sarah Spring’ area (7° 25′ 24″ S 107° 47′ 66″ W). Cells were irregular, motile cocci (0.8–1.5 µm in diameter) and divided by constriction. Growth was observed at temperatures between 60 °C and 95 °C with an optimum at 80 °C. The pH range for growth was between pH 4.0 and pH 8.0 with an optimum around pH 7.0. Strain Bio-pl-0405IT2T grew at salt concentrations of 1–5 % (w/v) NaCl with an optimum at 2 %. The novel isolate grew by fermentation or sulphur respiration on a variety of organic compounds. It was a chemoorganoheterotrophic archaeon growing preferentially with yeast extract, peptone and tryptone as carbon and energy sources and sulphur and organic compounds as electron acceptors; it also grew on maltose and starch. Sulphur or l-cystine were required for growth and were reduced to hydrogen sulfide. The strain was resistant to rifampicin, chloramphenicol, vancomycin and kanamycin (all at 100 µg ml−1) but was sensitive to tetracycline. The G+C content of its genomic DNA was 53.6 mol%. Phylogenetic analysis of the almost complete 16S rRNA gene sequence (1450 bp) of strain Bio-pl-0405IT2T showed that the novel isolate belonged to the genus Thermococcus . DNA–DNA hybridization values with the two closest relatives Thermococcus hydrothermalis AL662T and Thermococcus celer JCM 8558T were below the threshold value of 70 %. On the basis of the physiological and genotypic distinctness, we propose a novel species, Thermococcus prieurii sp. nov. The type strain is Bio-pl-0405IT2T ( = CSUR P577T = JCM 16307T).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic archaeon from a Siberian permafrost-affected soil
More LessA methanogenic archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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- Bacteroidetes
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Mucilaginibacter herbaticus sp. nov., isolated from the rhizosphere of the medicinal plant Angelica sinensis
More LessA strictly aerobic, Gram-staining-negative, non-motile and rod-shaped bacterial strain, DR-9T, was isolated from rhizosphere soil of the medicinal herb Angelica sinensis. Strain DR-9T grew at 20–40 °C, at pH 4.0–9.0 and in the presence of 0–1 % (w/v) NaCl. The major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH), MK-7 was the major isoprenoid quinone, and phosphatidylethanolamine and an unidentified aminophospholipid were the major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain DR-9T formed a lineage within the genus Mucilaginibacter and was closely related to Mucilaginibacter polysacchareus DRP28T (96.1 % sequence similarity), Mucilaginibacter myungsuensis HMD1056T (95.9 % sequence similarity), Mucilaginibacter ximonensis XM-003T (95.8 %) and Mucilaginibacter boryungensis BDR-9T (95.1 %). The status of strain DR-9T as a representative of a separate species was confirmed by DNA hybridization, with 38.6, 36.3 and 29.9 % DNA–DNA relatedness with M. polysacchareus DRP28T, M. ximonensis XM-003T and M. boryungensis BDR-9T, respectively. The genomic DNA G+C content of strain DR-9T was 49.8 %. These data suggest that strain DR-9T should be considered as a representative of a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter herbaticus sp. nov. is proposed. The type strain is DR-9T ( = KACC 16469T = NBRC 108839T).
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Pontibacter ramchanderi sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment
More LessA Gram-stain-negative, motile, red pigmented, rod-shaped bacterium, designated strain LP43T, was isolated from hexachlorocyclohexane (HCH)-contaminated soil sediment (Lucknow, India). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate formed a cluster with the genus Pontibacter in the phylum Bacteroidetes with sequence similarities ranging from 92.9 to 97.0 % with species of the genus Pontibacter . The DNA G+C content of strain LP43T was 59.1 mol%. The polar lipid profile of strain LP43T showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids and unknown polar lipids. Strain LP43T contained MK-7 as the predominant menaquinone and sym-homospermidine as the major polyamine. The major cellular fatty acids of strain LP43T were, iso-C15 : 0 (15.74 %), iso-C15 : 0 3-OH (7.57 %), iso-C17 : 0 3-OH (7.32 %), summed feature 4 (iso-C17 : 1 I/anteiso-C17 : 1 B) (31.22 %) and summed feature 8 (C18 : 1ω7c/ C18 : 1ω6c) (7.60 %). Based on the results of DNA–DNA hybridization and phenotypic and genotypic characteristics, strain LP43T represents a novel species of the genus Pontibacter , for which the name Pontibacter ramchanderi is proposed. The type strain is LP43T ( = CCM 8406T = MCC 2019T).
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Chryseobacterium kwangjuense sp. nov., isolated from pepper (Capsicum annuum L.) root
More LessThe yellow-pigmented, Gram-stain-negative, rod-shaped bacterium KJ1R5T was isolated from the root of a pepper plant grown in a field in Kwangju, Korea. Strain KJ1R5T was characterized by physiological, biochemical, and molecular genetic analyses. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KJ1R5T was most closely related to members of the genus Chryseobacterium , and that the strain exhibited the highest similarities with type strains of Chryseobacterium vrystaatense (97.0 %) and Chryseobacterium rhizosphaerae (97.1 %). DNA–DNA hybridization reassociation values between strain KJ1R5T and type strains of C. vrystaatense KACC 11675T and C. rhizosphaerae KACC 14918T were 46.9 and 38.4 %, respectively. The DNA G+C content of KJ1R5T is 40.2 mol%. The predominant respiratory quinone of KJ1R5T was menaquinone MK-6; major cellular fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 1ω9c, and iso-C17 : 0 3-OH. On the basis of these phenotypic and genotypic characteristics, the strain significantly differed from representative strains belonging to the genus Chryseobacterium . Thus, we propose that strain KJ1R5T represents a novel species of the genus Chryseobacterium , named Chryseobacterium kwangjuense sp. nov. The type strain is KJ1R5T ( = KACC 13029T = JCM 15904T).
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Flavobacterium cutihirudinis sp. nov., isolated from the skin of the medical leech Hirudo verbana
More LessA Gram-staining-negative, non-endospore-forming, yellow-pigmented strain (E89T) was isolated from the skin of the medical leech Hirudo verbana obtained from a leech farm located in Biebertal, Germany. 16S rRNA gene sequencing analysis showed that the isolate was grouped in the genus Flavobacterium . Strain E89T was most closely related to Flavobacterium chilense LM-09-FpT (98.2 %), Flavobacterium chungangense CJ7T (98.1 %), and Flavobacterium oncorhynchi 631-08T (98.1 %). 16S rRNA gene sequence similarities to all other species of the genus Flavobacterium were ≤97.4 %. A menaquinone of the type MK-6 was found to be the predominant respiratory quinone and the polar lipid profile consisted of the major compounds phosphatidylethanolamine, phosphatidylserine, two unidentified aminolipids, one unknown phospholipid and two unknown lipids. The fatty acid profile was composed of iso-C15 : 0, C15 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) found in major amounts and several hydroxylated fatty acids in smaller amounts, among them iso-C15 : 0 3-OH and iso-C17 : 0 3-OH. All these data support the allocation of the isolate in the genus Flavobacterium . Physiological/biochemical characterization and DNA–DNA hybridizations with the type strains of the most closely related species allowed a clear phenotypic and genotypic differentiation of the strain. Based on these data, strain E89T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium cutihirudinis sp. nov. is proposed. The type strain is E89T ( = DSM 25795T = LMG 26922T = CIP 110374T).
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Flavobacterium yanchengense sp. nov., isolated from soil
More LessA Gram-stain-negative, non-spore-forming, rod-shaped bacterial strain, hgT, resembling members of the genus Flavobacterium , was isolated from soil, and subjected to a taxonomic study using a polyphasic approach. Strain hgT grew optimally at pH 7.0 and 30 °C in the presence of 1 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The DNA G+C content was 34 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain hgT belonged to the genus Flavobacterium . Levels of 16S rRNA gene sequence similarity between strain hgT and the type strains of species of the genus Flavobacterium were below 94.7 %. Strain hgT differed from phylogenetically related species of the genus Flavobacterium in several phenotypic characteristics. On the basis of phenotypic and phylogenetic distinctiveness, strain hgT ( = CCTCC AB 2012099T = KACC 16855T) was classified in the genus Flavobacterium as the type strain of a novel species, for which the name Flavobacterium yanchengense sp. nov. is proposed.
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Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand
A yellow, rod-shaped, Gram-negative, facultatively aerobic, gliding bacterium, designed strain P7-3-5T, was isolated from intertidal sand of the Yellow Sea, China. Analysis of 16S rRNA gene sequences revealed that strain P7-3-5T formed a distinct lineage within the family Flavobacteriaceae , sharing 94.2–96.9 % sequence similarity with type strains of species of the most closely related genera, including Hyunsoonleella , Jejuia , Marinivirga and Algibacter . The strain grew at 4–40 °C and with 0.5–5.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed gelatin and DNA. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0 and the major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine (PE), three unidentified aminolipids (AL1–3) and four unidentified lipids (L1–4). The genomic DNA G+C content of strain P7-3-5T was 32.1 mol%. Data from this polyphasic study suggest that strain P7-3-5T represents a novel species in a new genus in the family Flavobacteriaceae , for which the name Arenitalea lutea gen. nov., sp. nov. is proposed. The type strain of Arenitalea lutea is P7-3-5T ( = CGMCC 1.12213T = KACC 16457T).
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Volumes and issues
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Volume 74 (2024)
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Volume 73 (2023)
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Volume 72 (2022 - 2023)
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Volume 71 (2020 - 2021)
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Volume 70 (2020)
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Volume 69 (2019)
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Volume 68 (2018)
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Volume 67 (2017)
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Volume 66 (2016)
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Volume 65 (2015)
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Volume 64 (2014)
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Volume 63 (2013)
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Volume 62 (2012)
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Volume 61 (2011)
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Volume 60 (2010)
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Volume 59 (2009)
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Volume 58 (2008)
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Volume 57 (2007)
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Volume 56 (2006)
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Volume 55 (2005)
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Volume 54 (2004)
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Volume 53 (2003)
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Volume 52 (2002)
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Volume 51 (2001)
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Volume 50 (2000)
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Volume 49 (1999)
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Volume 48 (1998)
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Volume 47 (1997)
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Volume 46 (1996)
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Volume 45 (1995)
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Volume 44 (1994)
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Volume 43 (1993)
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Volume 42 (1992)
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Volume 41 (1991)
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Volume 40 (1990)
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Volume 39 (1989)
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Volume 38 (1988)
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Volume 37 (1987)
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Volume 36 (1986)
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Volume 35 (1985)
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Volume 34 (1984)
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Volume 33 (1983)
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Volume 32 (1982)
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Volume 31 (1981)
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Volume 30 (1980)
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Volume 29 (1979)
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Volume 28 (1978)
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Volume 27 (1977)
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Volume 26 (1976)
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Volume 25 (1975)
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Volume 24 (1974)
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Volume 23 (1973)
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Volume 22 (1972)
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Volume 21 (1971)
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Volume 20 (1970)
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Volume 19 (1969)
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Volume 18 (1968)
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Volume 17 (1967)
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Volume 16 (1966)
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Volume 15 (1965)
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Volume 14 (1964)
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Volume 13 (1963)
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Volume 12 (1962)
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Volume 11 (1961)
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Volume 10 (1960)
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Volume 9 (1959)
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Volume 8 (1958)
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Volume 7 (1957)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)