- Volume 64, Issue Pt_5, 2014
Volume 64, Issue Pt_5, 2014
- New Taxa
-
- Proteobacteria
-
-
Sphingomonas oligoaromativorans sp. nov., an oligotrophic bacterium isolated from a forest soil
More LessA halo- and organo-sensitive oligotrophic bacterium, designated strain SY-6T, was isolated from humus forest soil at Gyeryong mountain in Korea. Cells of the strain were Gram-negative, strictly aerobic, non-motile rods and the strain formed yellow-pigmented colonies on 100-fold-diluted nutrient broth. Strain SY-6T grew at pH 6.0–7.0 (optimal growth at pH 7.0), at 10–37 °C (optimal growth at 28 °C) and at salinities of 0–0.5 % (w/v) NaCl, growing optimally at 0.01 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain SY-6T was shown to belong to the genus Sphingomonas and showed the closest phylogenetic similarity to Sphingomonas polyaromaticivorans B2-7T (96.7 %). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone and polyamine were Q-10 and sym-homospermidine, respectively. The major fatty acids were C18 : 1ω7c and C16 : 0. The DNA G+C content of the novel isolate was 65.3 mol%. On the basis of the evidence from this polyphasic study, strain SY-6T represents a novel species of the genus Sphingomonas , for which the name Sphingomonas oligoaromativorans sp. nov. is proposed. The type strain is SY-6T ( = KACC 12948T = NBRC 105508T).
-
-
-
Description of a Gram-negative bacterium, Sphingomonas guangdongensis sp. nov.
A Gram-stain-negative bacterial strain, designated 9NM-8T, was isolated from an abandoned lead-zinc ore in Mei county, Meizhou, Guangdong province, PR China. The isolate was orange-pigmented, aerobic, oxidase- and catalase-positive, motile with lophotrichous flagella and rod-shaped. Strain 9NM-8T grew optimally at pH 7.0 and 30 °C and in the absence of NaCl on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 9NM-8T belongs to the genus Sphingomonas , with highest sequence similarities to Sphingomonas azotifigens KACC 14484T (96.1 %), Sphingomonas trueperi DSM 7225T (96.0 %) and Sphingomonas pituitosa DSM 13101T (95.6 %). Strain 9NM-8T contained Q-10 as the predominant ubiquinone. The major fatty acids included C18 : 1ω7c, C16 : 0, C16 : 1ω7c and/or C16 : 1ω6c (summed feature 3) and 11-methyl C18 : 1ω7c. The DNA G+C content was 69.6±1.3 mol%. The major component in the polyamine pattern was sym-homospermidine and the polar lipid profile contained sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and two unidentified phospholipids. Based on comparative analysis of physiological, chemotaxonomic and phylogenetic characteristics, strain 9NM-8T should be considered to represent a novel species of the genus Sphingomonas , for which the name Sphingomonas guangdongensis sp. nov. is proposed. The type strain is 9NM-8T ( = GIMCC 1.653T = CGMCC 1.12672T = DSM 27570T).
-
-
-
Paenochrobactrum pullorum sp. nov. isolated from a chicken
More LessA Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (strain 280T) isolated from a chicken was studied for its taxonomic allocation. 16S rRNA gene sequence analyses clearly allocated the isolate in the genus Paenochrobactrum group with a 16S rRNA gene sequence similarity of 98.8 % to the currently recognized species, Paenochrobactrum gallinarii and Paenochrobactrum glaciei . This allocation was confirmed by the fatty acid data (major fatty acids: C18 : 1ω7c and C19 : 0 cyclo ω8c) and a polyamine pattern with the major compound putrescine and relatively high amounts of spermidine. Also, the polar lipid profile with phosphatidylethanolamine, phosphatiylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and the genus-specific ‘stretched aminolipid’ was well in line with the description of the genus Paenochrobactrum . The quinone system consisted predominantly of ubiquinone Q-10 with traces of Q-9 and Q-11. DNA–DNA hybridization of strain 280T with Paenochrobactrum gallinarii Sa25T and Paenochrobactrum glaciei KMM 3858T showed relatedness values of 38.8 % (reciprocal 20.2 %) and 30.2 % (reciprocal 29.8 %), respectively. These results in combination with differentiating physiological and biochemical data clearly showed that strain 280T merits species status. We propose the name Paenochrobactrum pullorum sp. nov. to accommodate this strain with the type strain 280T ( = LMG 28095T = CIP 110700T).
-
-
-
Roseivivax roseus sp. nov., an alphaproteobacterium isolated from a solar saltern soil sample
More LessA pink, Gram-stain-negative, motile, halotolerant bacterium with subpolar flagellum, designated strain BH87090T, was isolated from a saline soil sample collected from the south-west coastal area of South Korea (125° 58′ 58.08″ E 34° 45′ 37.32″ N). The isolate formed opaque pink to red colonies on marine agar plates at 30 °C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sulfoquinovosyl diacylglycerol, phosphatidylcholine and one unidentified phospholipid. The sole respiratory quinone was ubiquinone-10 (Q-10). The major cellular fatty acids were C18 : 1ω7c, C19 : 0 cyclo ω8c, C16 : 0 and 11-methyl C18 : 1ω7c. The genomic DNA G+C content was 61.8 mol%. These chemotaxonomic characteristics were all consistent with specific properties of the genus Roseivivax . Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate affiliated to the cluster with members of the genus Roseivivax in the Roseobacter clade, which suggested that the strain belonged to the genus Roseivivax . However, the low 16S rRNA gene similarities (93.5–95.3 %) of strain BH87090T with all the members of the genus Roseivivax indicated that it represented a novel species of the genus Roseivivax . On the basis of phenotypic and genotypic data, strain BH87090T should be classified as a novel species of the genus Roseivivax . The name Roseivivax roseus sp. nov. is proposed, with strain BH87090T ( = DSM 23042T = KCTC 22650T) as the type strain.
-
-
-
Psychroglaciecola arctica gen. nov., sp. nov., isolated from Arctic glacial foreland soil
More LessA novel pink-pigmented, facultatively methylotrophic strain, designated M6-76T, was isolated from glacial foreland soil near Ny-Ålesund, Svalbard Archipelago, Norway. Cells of strain M6-76T were rod-shaped (0.4–0.7×0.8–2.0 µm), Gram-stain-negative, aerobic and motile by a single polar flagellum. Growth occurred at 4–28 °C (optimum 18 °C) and at pH 5–8 (optimum pH 7). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M6-76T belonged to the family Methylobacteriaceae . The 16S rRNA gene sequence of the novel strain showed 94.6 %, 94.0 % and 93.9 % sequence similarity to those of Methylobacterium salsuginis MRT, Methylobacterium organophilum ATCC 27886T and Microvirga subterranea FaiI4T, respectively. Cells could utilize methanol as the sole source of carbon and energy but not formate. The major respiratory quinone was Q-10. The polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and two unknown polar lipids. The predominant cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and C16 : 0. The DNA G+C content was 67 mol%. The polyphasic data presented in this study indicated that the isolate should be classified as representing a novel species of a new genus within the family Methylobacteriaceae . The name Psychroglaciecola arctica gen. nov., sp. nov. is therefore proposed for the isolate. The type strain of the type species is M6-76T ( = CCTCC AB 2013033T = KACC 17684T).
-
- Bacteroidetes
-
-
Moheibacter sediminis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from sediment, and emended descriptions of Empedobacter brevis, Wautersiella falsenii and Weeksella virosa
More LessA Gram-reaction-negative, yellow-pigmented, strictly aerobic bacterium, designated M0116T, was isolated from the sediment of the Mohe Basin in north-east China. Flexirubin-type pigments were produced. Cells were catalase- and oxidase-positive and non-gliding rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0116T was a member of the family Flavobacteriaceae and was most closely related to members of the genera Empedobacter , Wautersiella and Weeksella with 90.5–91.0 % sequence similarities. The major cellular fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain M0116T is considered to represent a novel species of a new genus in the family Flavobacteriaceae , for which the name Moheibacter sediminis gen. nov., sp. nov. is proposed. The type strain is M0116T ( = CGMCC 1.12708T = JCM 19634T). Emended descriptions of Empedobacter brevis , Wautersiella falsenii and Weeksella virosa are also proposed.
-
-
-
Flavobacterium longum sp. nov. and Flavobacterium urocaniciphilum sp. nov., isolated from a wastewater treatment plant, and emended descriptions of Flavobacterium caeni and Flavobacterium terrigena
More LessTwo Gram-staining-negative, strictly aerobic, non-endospore-forming, non-motile, rod-shaped bacteria, designated strains YIT 12745T and YIT 12746T, were isolated from sludge from a wastewater treatment plant. 16S rRNA gene sequence analyses indicated that these strains belonged to the genus Flavobacterium . In these analyses, strains YIT 12745T and YIT 12746T were most closely related to the type strains of Flavobacterium caeni and Flavobacterium terrigena , with 16S rRNA gene sequence similarity values of 94.9 % and 96.2 %, respectively. For both novel strains, menaquinone (MK-6) was the only respiratory quinone. The major fatty acids of strain YIT 12745T were iso-C15 : 1 G (14.4 %), iso-C16 : 0 (13.2 %), C15 : 0 (12.9 %), iso-C15 : 0 (12.9 %) and iso-C17 : 0 3-OH (11.5 %). Those of strain YIT 12746T were iso-C15 : 0 (21.5 %), iso-C16 : 0 (13.3 %), C15 : 0 (12.0 %) and iso-C15 : 1 G (11.9 %). The genomic DNA G+C contents of strains YIT 12745T and YIT 12746T were 48.7 and 30.9 mol%, respectively. From their differential phenotypic and phylogenetic characteristics, these strains are considered to represent two novel species of the genus Flavobacterium , for which the names Flavobacterium longum sp. nov. (type strain YIT 12745T = JCM 19141T = DSM 27077T) and Flavobacterium urocaniciphilum sp. nov. (type strain YIT 12746T = JCM 19142T = DSM 27078T) are proposed. Emended descriptions of Flavobacterium caeni and Flavobacterium terrigena are also proposed.
-
-
-
Salegentibacter chungangensis sp. nov., isolated from a sea sand and emended description of the genus Salegentibacter
More LessA Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterial strain, designated CAU 1289T, was isolated from a marine sand and its taxonomic position was investigated using a polyphasic approach. It grew optimally at pH 6.5 and 30 °C and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1289T belonged to the genus Salegentibacter , exhibiting sequence similarity values of 93.6–96.3 % to members of this genus, and was related most closely to Salegentibacter mishustinae KMM 6049T (96.3 % similarity). Strain CAU 1289T contained MK-6 as the predominant menaquinone. The major fatty acid was iso-C15 : 0. The cell-wall peptidoglycan of strain CAU 1289T contained meso-diaminopimelic acid. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine, an unidentified phospholipid, an unidentified aminolipid, an unidentified aminophospholipid, an unidentified glycolipid and ten unidentified lipids. The DNA G+C content was 38.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic inference, strain CAU 1289T is considered to represent a novel species of the genus Salegentibacter , for which the name Salegentibacter chungangensis sp. nov. is proposed. The type strain is CAU 1289T (KCTC 24000T = CCUG 64793T). An emended description of the genus Salegentibacter is also proposed.
-
-
-
Winogradskyella wandonensis sp. nov., isolated from a tidal flat
More LessA Gram-stain-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated WD-2-2T, was isolated from a tidal flat of Wando, an island of South Korea, and subjected to a polyphasic taxonomic analysis. Strain WD-2-2T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WD-2-2T belonged to the genus Winogradskyella , clustering coherently with the type strain of Winogradskyella litorisediminis . Strain WD-2-2T exhibited 16S rRNA gene sequence similarity of 97.4 % to W. litorisediminis DPS-8T and 94.5–96.6 % to the type strains of the other species of the genus Winogradskyella . Strain WD-2-2T contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C17 : 0 3-OH and iso-C15 : 0 as the major fatty acids. The major polar lipids detected in strain WD-2-2T were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content was 36.4 mol%, and DNA–DNA relatedness with W. litorisediminis DPS-8T was 13 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain WD-2-2T is separate from recognized species of the genus Winogradskyella . On the basis of the data presented, strain WD-2-2T is considered to represent a novel species of the genus Winogradskyella , for which the name Winogradskyella wandonensis sp. nov. is proposed. The type strain is WD-2-2T ( = KCTC 32579T = CECT 8445T).
-
-
-
Chryseobacterium aahli sp. nov., isolated from lake trout (Salvelinus namaycush) and brown trout (Salmo trutta), and emended descriptions of Chryseobacterium ginsenosidimutans and Chryseobacterium gregarium
More LessTwo strains (T68T and T62) of a Gram-reaction-negative, yellow-pigmented bacterium containing flexirubin-type pigments were recovered from the kidney of a cultured lake trout (Salvelinus namaycush) and necrotic fins of a brown trout (Salmo trutta) during disease surveillance in 2009. Both isolates possessed catalase and cytochrome oxidase activities and degraded multiple substrates (e.g. gelatin, casein, elastin and Tweens 20 and 80). The mean DNA G+C content of strain T68T was 34.1 mol%. 16S rRNA gene sequencing demonstrated that strains T68T and T62 had nearly identical sequences (≥99 % similarity) and placed the bacterium within the genus Chryseobacterium , where Chryseobacterium ginsenosidimutans THG 15T (97.8 %), C. gregarium DSM 19109T (97.7 %) and C. soldanellicola PSD1-4T (97.6 %) were its closest relatives. Subsequent phylogenetic analyses using neighbour-joining, maximum-parsimony and Bayesian methodologies demonstrated that strains T68T and T62 formed a well-supported clade (bootstrap values of 100 and 97 %; posterior probability 0.99) that was distinct from other species of the genus Chryseobacterium . The major fatty acids of strains T68T and T62 were characteristic of the genus Chryseobacterium and included iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), iso-C17 : 0 3-OH, C16 : 0 and C16 : 0 3-OH. The mean DNA–DNA relatedness of strain T68T to C. ginsenosidimutans JCM 16719T and C. gregarium LMG 24952T was 24 and 21 %, respectively. Based on the results from our polyphasic characterization, strains T68T and T62 represent a novel species of the genus Chryseobacterium , for which the name Chryseobacterium aahli sp. nov. is proposed. The type strain is T68T ( = LMG 27338T = ATCC BAA-2540T). Emended descriptions of Chryseobacterium ginsenosidimutans and Chryseobacterium gregarium are also proposed.
-
-
-
Polaribacter atrinae sp. nov., isolated from the intestine of a comb pen shell, Atrina pectinata
A novel Gram-staining-negative, aerobic, non-motile, yellow-to-orange carotenoid-type-pigmented and rod-shaped bacterium, designated strain WP25T, was isolated from the intestine of a comb pen shell, Atrina pectinata, which was collected from the South Sea near Yeosu in Korea. The isolate grew optimally at 20 °C, at pH 7 and with 2 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain WP25T belonged to the genus Polaribacter in the family Flavobacteriaceae and the highest sequence similarity was shared with the type strain of Polaribacter sejongensis (98.5 %). The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, C15 : 1ω6c and iso-C15 : 0 3-OH. The main respiratory quinone was menaquinone MK-6. The polar lipids of strain WP25T were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and four unidentified lipids. The genomic DNA G+C content was 31.2 mol%. DNA–DNA hybridization experiments indicated <12.6 % genomic relatedness with closely related strains. Based on phylogenetic, phenotypic and genotypic analyses, strain WP25T represents a novel species in the genus Polaribacter , for which the name Polaribacter atrinae sp. nov. is proposed, with the type strain WP25T ( = KACC 17473T = JCM 19202T).
-
-
-
Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov.
More LessThe taxonomic characteristics of two bacterial strains, FH5T and SS4, isolated from enrichment cultures obtained from two distinct marine environments, were determined. These bacteria were Gram-stain-negative, facultatively anaerobic rods. Growth occurred at 20–40 °C (optimum, 28–32 °C), pH 5.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 1–7 % NaCl (optimum, 2–4 %). The major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0. Menaquinone 7 (MK-7) was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, an unkown phospholipid and an unknown lipid. The DNA G+C contents of strains FH5T and SS4 were both determined to be 42.0 mol%. The results of DNA–DNA hybridization studies indicated that the FH5T and SS4 genomes share greater than 95 % relatedness. The strains formed a distinct phyletic line within the class Bacteroidia , with less than 89.4 % sequence similarity to their closest relatives with validly published names. On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, Draconibacterium orientale gen. nov., sp. nov., within the class Bacteroidia , are proposed, with strain FH5T ( = DSM 25947T = CICC 10585T) as the type strain. In addition, a new family, Draconibacteriaceae fam. nov., is proposed to accommodate Draconibacterium gen. nov.
-
-
-
Mucilaginibacter auburnensis sp. nov., isolated from a plant stem
More LessA yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1070T) was isolated as a presumptive endophyte from internal stem tissue of a healthy corn stem. Phylogenetic analyses based on the 16S rRNA gene sequence placed strain JM-1070T in the monophyletic cluster of the genus Mucilaginibacter , with closest affiliation to the type strains of Mucilaginibacter composti (98 % similarity) and Mucilaginibacter calamicampi (97.2 %). 16S rRNA gene sequence similarity to type strains of other species of the genus Mucilaginibacter was 93.4–96.0 %. DNA–DNA hybridizations between strain JM-1070T and the type strains M. composti LMG 23497T and M. calamicampi CCUG 63418T showed low relatedness values of 13 % (reciprocal 18 %) and 52 % (reciprocal 54.4 %). Major respiratory quinones were menaquinones MK-6 and MK-7. The predominant fatty acids (>15 %) were iso-C15 : 0, iso-C15 : 0 2-OH/C16 : 1ω7c (measured as summed feature 3) and iso-C17 : 0 3-OH. Several other iso-branched and hydroxylated fatty acids were detected. The polar lipid profile was composed of the major components phosphatidylethanolamine and an unidentified aminophospholipid. The polyamine pattern contained predominantly sym-homospermidine. Characterization by 16S rRNA gene sequencing, physiological parameters and polyamine, ubiquinone, polar lipid and fatty acid compositions revealed that strain JM-1070T represents a novel species of the genus Mucilaginibacter . For this reason, we propose the name Mucilaginibacter auburnensis sp. nov., with the type strain JM-1070T ( = CIP 110694T = LMG 28078T).
-
-
-
Bacteroides luti sp. nov., an anaerobic, cellulolytic and xylanolytic bacterium isolated from methanogenic sludge
More LessA mesophilic, anaerobic, cellulolytic and xylanolytic strain, UasXn-3T, was isolated from anaerobic granular sludge in a mesophilic upflow anaerobic sludge blanket reactor, which was used to treat municipal sewage. The cells were Gram-stain-negative, non-motile, and non-spore-forming rods. The optimal temperature for growth was 37–40 °C and the optimal pH for growth was pH 6.5–7.0. Strain UasXn-3T could grow on several polysaccharides and sugars, including cellulose, cellobiose, xylan, xylose, glucose, fructose, arabinose, mannose, raffinose, trehalose and starch. The DNA G+C content was 44.4 mol%. On the basis of comparative 16S rRNA gene sequence analysis, strain UasXn-3T was identified as a member of the genus Bacteroides and most closely related to Bacteroides oleiciplenus , B. intestinalis , B. cellulosilyticus and B. graminisolvens (sequence similarities of 91.3–91.6 %). Since the genetic and phenotypic properties suggest that strain UasXn-3T represents a novel species, we propose the name Bacteroides luti sp. nov. The type strain is UasXn-3T ( = JCM 19020T = DSM 26991T).
-
-
-
Pedobacter jejuensis sp. nov., isolated from soil of a pine grove, and emended description of the genus Pedobacter
More LessA Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, THG-DR3T, was isolated from soil of a pine grove in Jeju island, Republic of Korea. Strain THG-DR3T grew optimally at 20–25 °C, at pH 7.0–8.0 and in R2A broth without added NaCl. 16S rRNA gene sequence analysis indicated that strain THG-DR3T belongs to the genus Pedobacter . The closest relatives based on 16S rRNA gene sequence similarity were Pedobacter aquatilis AR107T (98.0 %), Pedobacter alluvionis NWER-II11T (97.7 %), Pedobacter borealis G-1T (97.7 %) and Pedobacter ginsengiterrae DCY49T (97.4 %). The G+C content of the genomic DNA of strain THG-DR3T was 47.5 mol%. Levels of DNA–DNA relatedness between strain THG-DR3T and its closest phylogenetic neighbours were below 44.7 %. The presence of menaquinone 7 and of iso-C15 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids supported the affiliation of strain THG-DR3T to the genus Pedobacter . The polar lipids of strain THG-DR3T consisted of phosphatidylethanolamine, three unidentified aminophospholipids, five unidentified aminolipids and three unidentified lipids. The results of physiological and biochemical tests allowed the differentiation of strain THG-DR3T from recognized species of the genus Pedobacter . Strain THG-DR3T ( = KACC 17172T = JCM 18824T) is proposed as the type strain of a novel species of the genus Pedobacter , Pedobacter jejuensis sp. nov. An emended description of the genus Pedobacter is also proposed.
-
-
-
Taibaiella chishuiensis sp. nov., isolated from freshwater
More LessA Gram-reaction-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, AY17T, was isolated from the Chishui River in Guizhou Province, South-west China. Strain AY17T grew optimally at pH 7.0 and 20 °C. Flexirubin-type pigments were produced. 16S rRNA gene sequence analysis indicated that strain AY17T belonged to the family Chitinophagaceae within the phylum Bacteroidetes ; the closest phylogenetic relative was Taibaiella smilacinae PTJT-5T (95.3 % gene sequence similarity). The DNA G+C content was 49.5 mol%. Strain AY17T contained MK-7 as the predominant respiratory quinone and phosphatidylethanolamine as the major polar lipid. The major fatty acids were iso-C15 : 0, iso-C15 : 1G and iso-C17 : 0 3-OH. On the basis of phylogenetic, phenotypic and genetic data, strain AY17T was classified as representing a novel species of the genus Taibaiella , for which the name Taibaiella chishuiensis sp. nov. is proposed. The type strain is AY17T ( = CGMCC 1.12700T = JCM 19637T).
-
- Other Bacteria
-
-
Terrimicrobium sacchariphilum gen. nov., sp. nov., an anaerobic bacterium of the class ‘Spartobacteria’ in the phylum Verrucomicrobia, isolated from a rice paddy field
More LessA strictly anaerobic, mesophilic, carbohydrate-fermenting bacterium, designated NM-5T, was isolated from a rice paddy field. Cells of strain NM-5T were Gram-stain-negative, non-motile, non-spore-forming, short rods (0.5–0.7 µm×0.6–1.2 µm). The strain grew optimally at 37 °C (growth range 20–40 °C) and pH 7.0 (pH 5.5–8.0). The strain could grow fermentatively on arabinose, xylose, fructose, galactose, glucose, ribose, mannose, cellobiose, lactose, maltose and sucrose. The main end-products of glucose fermentation were acetate and propionate. Organic acids, alcohols and amino acids were not utilized for growth. Yeast extract was not required but stimulated the growth. Nitrate, sulfate, thiosulfate, elemental sulfur, sulfite, and Fe (III) nitrilotriacetate were not used as terminal electron acceptors. The DNA G+C content was 46.3 mol%. The major cellular fatty acids were iso-C14 : 0, C18 : 0 and C16 : 0. 16S rRNA gene sequence analysis revealed that strain NM-5T belongs to the class ‘S partobacteria’, subdivision 2 of the bacterial phylum Verrucomicrobia . Phylogenetically, the closest species was ‘Chthoniobacter flavus’ (89.6 % similarity in 16S rRNA gene sequence). A novel genus and species, Terrimicrobium sacchariphilum gen. nov., sp. nov., is proposed. The type strain of the type species is NM-5T ( = JCM 17479T = CGMCC 1.5168T).
-
- Erratum
-
Volumes and issues
-
Volume 74 (2024)
-
Volume 73 (2023)
-
Volume 72 (2022 - 2023)
-
Volume 71 (2020 - 2021)
-
Volume 70 (2020)
-
Volume 69 (2019)
-
Volume 68 (2018)
-
Volume 67 (2017)
-
Volume 66 (2016)
-
Volume 65 (2015)
-
Volume 64 (2014)
-
Volume 63 (2013)
-
Volume 62 (2012)
-
Volume 61 (2011)
-
Volume 60 (2010)
-
Volume 59 (2009)
-
Volume 58 (2008)
-
Volume 57 (2007)
-
Volume 56 (2006)
-
Volume 55 (2005)
-
Volume 54 (2004)
-
Volume 53 (2003)
-
Volume 52 (2002)
-
Volume 51 (2001)
-
Volume 50 (2000)
-
Volume 49 (1999)
-
Volume 48 (1998)
-
Volume 47 (1997)
-
Volume 46 (1996)
-
Volume 45 (1995)
-
Volume 44 (1994)
-
Volume 43 (1993)
-
Volume 42 (1992)
-
Volume 41 (1991)
-
Volume 40 (1990)
-
Volume 39 (1989)
-
Volume 38 (1988)
-
Volume 37 (1987)
-
Volume 36 (1986)
-
Volume 35 (1985)
-
Volume 34 (1984)
-
Volume 33 (1983)
-
Volume 32 (1982)
-
Volume 31 (1981)
-
Volume 30 (1980)
-
Volume 29 (1979)
-
Volume 28 (1978)
-
Volume 27 (1977)
-
Volume 26 (1976)
-
Volume 25 (1975)
-
Volume 24 (1974)
-
Volume 23 (1973)
-
Volume 22 (1972)
-
Volume 21 (1971)
-
Volume 20 (1970)
-
Volume 19 (1969)
-
Volume 18 (1968)
-
Volume 17 (1967)
-
Volume 16 (1966)
-
Volume 15 (1965)
-
Volume 14 (1964)
-
Volume 13 (1963)
-
Volume 12 (1962)
-
Volume 11 (1961)
-
Volume 10 (1960)
-
Volume 9 (1959)
-
Volume 8 (1958)
-
Volume 7 (1957)
-
Volume 6 (1956)
-
Volume 5 (1955)
-
Volume 4 (1954)
-
Volume 3 (1953)
-
Volume 2 (1952)
-
Volume 1 (1951)