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Volume 168, Issue 12

Small multidrug resistance protein EmrE phenotypically associates with OmpW, DcrB and YggM for osmotic stress protection by betaine in Free

This article is part of the Antimicrobial Efflux collection.

Abstract

The small multidrug resistance (SMR) protein EmrE resides in the inner membrane and provides resistance against a wide range of antiseptic quaternary cationic compounds (QCCs) for the Gram-negative bacterium . We have reported previously that overexpression of the gene results in the reduction of pH and osmotic tolerance, likely through EmrE-mediated biological QCC-based osmoprotectant efflux, indicating a potential physiological role for EmrE beyond providing drug resistance. EmrE is the most studied member of SMR transporter family; however, it is not known how the substrates translocated by EmrE move across the periplasm and through the outer membrane (OM). We have shown that the OM protein OmpW participates in the EmrE-mediated substrate efflux process and provided a hypothesis for the present study that additional OM and periplasmic proteins participate in the translocation process. To test the hypothesis, we conducted alkaline pH-based growth phenotype screens under overexpression conditions. This screen identified 10 additional genes that appear to contribute to the EmrE-coupled osmoprotectant efflux: , , , , , , , , and . Further screening of these genes using a hyperosmotic growth phenotype assay in the presence and the absence of the osmoprotectant glycine betaine identified and two periplasmic protein genes, and , are mechanistically linked to EmrE.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): EmrE , glycine betaine , osmoprotectants , outer membrane , QCC and small multidrug resistance
Funding
This study was supported by the:
  • NSERC
    • Principle Award Recipient: RaymondJ Turner
  • NSERC
    • Principle Award Recipient: DeniceC. Bay
© 2022 The Authors
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2022-12-19
2024-05-08
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Small multidrug resistance protein EmrE phenotypically associates with OmpW, DcrB and YggM for osmotic stress protection by betaine in Escherichia coli
Microbiology 168, 001287 (2022); https://doi.org/10.1099/mic.0.001287
/content/journal/micro/10.1099/mic.0.001287
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