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Volume 65, Issue Pt_3

Seed-associated subspecies of the genus are clearly distinguishable from subsp. Free

Abstract

The genus contains one recognized species, . is subdivided into subspecies based on host specificity and bacteriological characteristics, with causing bacterial canker of tomato. is often spread through contaminated seed leading to outbreaks of bacterial canker in tomato production areas worldwide. The frequent occurrence of non-pathogenic like bacteria (CMB) is a concern for seed producers because is a quarantine organism and detection of a non-pathogenic variant may result in destruction of an otherwise healthy seed lot. A thorough biological and genetic characterization of these seed-associated CMB strains was performed using standard biochemical tests, cell wall analyses, metabolic profiling using Biolog, and single-gene and multilocus sequence analyses. Combined, these tests revealed two distinct populations of seed-associated members of the genus that differed from each other, as well as from all other described subspecies of . DNA–DNA hybridization values are 70 % or higher, justifying placement into the single recognized species, , but other analyses justify separate subspecies designations. Additionally, strains belonging to the genus isolated from pepper also represent a distinct population and warrant separate subspecies designation. On the basis of these data we propose subspecies designations for separate non-pathogenic subpopulations of : subsp. nov. and subsp. nov. for seed-associated strains represented by C55 ( = ATCC BAA-2691 = CFBP 8216) and ZUM3936 ( = ATCC BAA-2690 = CFBP 8217), respectively. Recognition of separate subspecies is essential for improved international seed testing operations.

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Funding
This study was supported by the:
  • USDA-NIFA (Award SCRI-600-25320)
© 2015 IUMS
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2015-03-01
2024-04-24
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Seed-associated subspecies of the genus Clavibacter are clearly distinguishable from Clavibacter michiganensis subsp. michiganensis
Int J Syst Evol Microbiol 65, 811 (2015); https://doi.org/10.1099/ijs.0.000022
/content/journal/ijsem/10.1099/ijs.0.000022
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