1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_3

sp. nov., isolated from an inland salt lake, and emended description of the genus Free

Abstract

The halophilic archaeal strain, YC82, was isolated from Yuncheng salt lake in Shanxi, PR China. Cells from strain YC82 were Gram-stain negative, pleomorphic rods, which lysed in distilled water and formed light-red colonies on solid media. Strain YC82 grew at 25–50 °C (optimum 37 °C), in 1.4–4.8 M NaCl (optimum 2.0 M), in 0–1.0 M MgCl (optimum 0.05 M) and at pH 6.0–9.5 (optimum pH 7.5). The major polar lipids of strain YC82 were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids, which were chromatographically identical to those of IBRC-M 10013 and CGMCC 1.10390. 16S rRNA gene analysis revealed that strain YC82 had two dissimilar 16S rRNA genes and that it was phylogenetically related to IBRC-M 10013 (94.3–99.0 % nucleotide identity) and CGMCC 1.10390 (94.1–98.8 % nucleotide identity). The gene similarities between strain YC82 and IBRC-M 10013 and CGMCC 1.10390 were 96.5 % and 95.7 %, respectively. The DNA G+C content of strain YC82 was 63.7 mol%. Strain YC82 showed low DNA–DNA relatedness with IBRC-M 10013 and CGMCC 1.10390. The phenotypic, chemotaxonomic and phylogenetic properties of strain YC82 ( = CGMCC 1.12232 = JCM 18644) suggest that it represents a novel species of the genus , for which the name sp. nov. is proposed. An emended description of the genus is also presented.

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31370054)
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-15-R-03)
  • Qinglan Project of Jiangsu Province
  • Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000038
2015-03-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/3/910.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000038&mimeType=html&fmt=ahah

References

  1. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical methods in bacterial systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  2. Cui H.-L., Zhou P.-J., Oren A., Liu S.-J. ( 2009 ). Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium . . Extremophiles 13, 31–37. [View Article] [PubMed]
    [Google Scholar]
  3. Cui H.-L., Gao X., Yang X., Xu X.-W. ( 2010 ). Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. . Extremophiles 14, 493–499. [View Article] [PubMed]
    [Google Scholar]
  4. Cui H.-L., Yang X., Mou Y. Z. ( 2011 ). Salinarchaeum laminariae gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from salted brown alga Laminaria . . Extremophiles 15, 625–631. [View Article] [PubMed]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  6. Huß V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  7. Makhdoumi-Kakhki A., Amoozegar M. A., Bagheri M., Ramezani M., Ventosa A. ( 2012 ). Haloarchaeobius iranensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from a saline lake. . Int J Syst Evol Microbiol 62, 1021–1026. [View Article] [PubMed]
    [Google Scholar]
  8. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109–118. [View Article] [PubMed]
    [Google Scholar]
  9. Minegishi H., Kamekura M., Itoh T., Echigo A., Usami R., Hashimoto T. ( 2010 ). Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B′ (rpoB′) gene. . Int J Syst Evol Microbiol 60, 2398–2408. [View Article] [PubMed]
    [Google Scholar]
  10. Oren A. ( 2012 ). Taxonomy of the family Halobacteriaceae: a paradigm for changing concepts in prokaryote systematics. . Int J Syst Evol Microbiol 62, 263–271. [View Article] [PubMed]
    [Google Scholar]
  11. Oren A. ( 2014 ). Taxonomy of halophilic Archaea: current status and future challenges. . Extremophiles 18, 825–834. [View Article] [PubMed]
    [Google Scholar]
  12. Oren A., Ventosa A., Grant W. D. ( 1997 ). Proposed minimal standards for description of new taxa in the order Halobacteriales . . Int J Syst Bacteriol 47, 233–238. [View Article]
    [Google Scholar]
  13. Oren A., Arahal D. R., Ventosa A. ( 2009 ). Emended descriptions of genera of the family Halobacteriaceae . . Int J Syst Evol Microbiol 59, 637–642. [View Article] [PubMed]
    [Google Scholar]
  14. Qiu X.-X., Mou Y.-Z., Zhao M.-L., Zhang W.-J., Han D., Ren M., Cui H.-L. ( 2013 ). Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China. . Int J Syst Evol Microbiol 63, 3975–3980. [View Article] [PubMed]
    [Google Scholar]
  15. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  17. Youssef N. H., Ashlock-Savage K. N., Elshahed M. S. ( 2012 ). Phylogenetic diversities and community structure of members of the extremely halophilic Archaea (order Halobacteriales) in multiple saline sediment habitats. . Appl Environ Microbiol 78, 1332–1344. [View Article] [PubMed]
    [Google Scholar]
  18. Zhang W.-J., Cui H.-L. ( 2014 ). Haloarchaeobius litoreus sp. nov., isolated from a marine solar saltern. . Antonie van Leeuwenhoek 105, 1085–1090. [View Article] [PubMed]
    [Google Scholar]
  19. Zhaxybayeva O., Stepanauskas R., Mohan N. R., Papke R. T. ( 2013 ). Cell sorting analysis of geographically separated hypersaline environments. . Extremophiles 17, 265–275. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000038
Loading
Haloarchaeobius salinus sp. nov., isolated from an inland salt lake, and emended description of the genus Haloarchaeobius
Int J Syst Evol Microbiol 65, 910 (2015); https://doi.org/10.1099/ijs.0.000038
/content/journal/ijsem/10.1099/ijs.0.000038
/content/journal/ijsem/10.1099/ijs.0.000038
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error