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Volume 59, Issue 1

Description of seven candidate species affiliated with the phylum , representing planktonic freshwater bacteria Free

Abstract

Actinobacteria comprise a substantial fraction of the bacterioplankton in freshwater lakes and streams. Numerous cultivation-independent investigations have retrieved actinobacterial 16S rRNA gene sequences from such habitats. The taxa detected in freshwater habitats are usually absent from terrestrial and marine systems. So far, none of the indigenous freshwater lineages is represented by a taxon with a validly published name. The seven organisms for which status is described here were isolated from freshwater lakes and ponds located in tropical, subtropical and temperate climatic zones. Phylogenetic analyses demonstrated that they are affiliated with one of the actinobacterial lineages indigenous to freshwater bacterioplankton. The seven novel taxa could only be cultivated to date as mixed cultures that also contain non-actinobacterial strains. Due to the lack of pure cultures, I propose to establish the candidate species ‘ Planktoluna difficilis’, ‘ Aquiluna rubra’, ‘ Flaviluna lacus’, ‘ Rhodoluna limnophila’, ‘ Rhodoluna planktonica’, ‘ Rhodoluna lacicola’ and ‘ Limnoluna rubra’ for these taxa.

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Keyword(s): FISH, fluorescent in situ hybridization
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2009-01-01
2024-04-20
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References

  1. Allgaier, M. & Grossart, H.-P.(2006). Diversity and seasonal dynamics of Actinobacteria populations in four lakes in northeastern Germany. Appl Environ Microbiol 72, 3489–3497.[CrossRef] [Google Scholar]
  2. Beier, S., Witzel, K. P. & Marxsen, J.(2008). Bacterial community composition in Central European running waters examined by temperature gradient gel electrophoresis and sequence analysis of 16S rRNA genes. Appl Environ Microbiol 74, 188–199.[CrossRef] [Google Scholar]
  3. Crump, B. C. & Hobbie, J. E.(2005). Synchrony and seasonality in bacterioplankton communities of two temperate rivers. Limnol Oceanogr 50, 1718–1729.[CrossRef] [Google Scholar]
  4. Glöckner, F. O., Zaichikov, E., Belkova, N., Denissova, L., Pernthaler, J., Pernthaler, A. & Amann, R.(2000). Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of actinobacteria. Appl Environ Microbiol 66, 5053–5065.[CrossRef] [Google Scholar]
  5. Hahn, M. W. & Pöckl, M.(2005). Ecotypes of planktonic actinobacteria with identical 16S rRNA genes adapted to thermal niches in temperate, subtropical, and tropical freshwater habitats. Appl Environ Microbiol 71, 766–773.[CrossRef] [Google Scholar]
  6. Hahn, M. W., Lünsdorf, H., Wu, Q., Schauer, M., Höfle, M. G., Boenigk, J. & Stadler, P.(2003). Isolation of novel ultramicrobacteria classified as actinobacteria from five freshwater habitats in Europe and Asia. Appl Environ Microbiol 69, 1442–1451.[CrossRef] [Google Scholar]
  7. Hahn, M. W., Stadler, P., Wu, Q. L. & Pöckl, M.(2004). The filtration-acclimatization-method for isolation of an important fraction of the not readily cultivable bacteria. J Microbiol Methods 57, 379–390.[CrossRef] [Google Scholar]
  8. Jezbera, J., Horňák, K. & Šimek, K.(2005). Food selection by bacterivorous protists: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization. FEMS Microbiol Ecol 52, 351–363.[CrossRef] [Google Scholar]
  9. Murray, R. G. E. & Stackebrandt, E.(1995). Taxonomic note: implementation of the provisional status Candidatus for incompletely described procaryotes. Int J Syst Bacteriol 45, 186–187.[CrossRef] [Google Scholar]
  10. Rheinheimer, G.(1980).Aquatic Microbiology, 2nd edn. New York: Wiley.
  11. Rosselló-Mora, R. & Amann, R.(2001). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef] [Google Scholar]
  12. Sekar, R., Pernthaler, A., Pernthaler, J., Warnecke, F., Posch, T. & Amann, R.(2003). An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization. Appl Environ Microbiol 69, 2928–2935.[CrossRef] [Google Scholar]
  13. Sharma, A. K., Zhaxybayeva, O., Papke, R. T. & Doolittle, W. F.(2008). Actinorhodopsins: proteorhodopsin-like gene sequences found predominantly in non-marine environments. Environ Microbiol 10, 1039–1056.[CrossRef] [Google Scholar]
  14. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155. [Google Scholar]
  15. Warnecke, F., Amann, R. & Pernthaler, J.(2004). Actinobacterial 16S rRNA genes from freshwater habitats cluster in four distinct lineages. Environ Microbiol 6, 242–253.[CrossRef] [Google Scholar]
  16. Wu, Q. L. & Hahn, M. W.(2006). Differences in structure and dynamics of Polynucleobacter communities in a temperate and a subtropical lake revealed at three phylogenetic levels. FEMS Microbiol Ecol 57, 67–79.[CrossRef] [Google Scholar]
  17. Wu, Q. L., Zwart, G., Wu, J., Kamst-van Agterveld, M. P., Liu, S. & Hahn, M. W.(2007). Submersed macrophytes play a key role in structuring bacterioplankton community composition in the large, shallow, subtropical Taihu Lake, China. Environ Microbiol 9, 2765–2774.[CrossRef] [Google Scholar]
  18. Zwart, G., Crump, B. C., Kamst-van Agterveld, M. P., Hagen, F. & Han, S.-K.(2002). Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers. Aquat Microb Ecol 28, 141–155.[CrossRef] [Google Scholar]
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Description of seven candidate species affiliated with the phylum Actinobacteria, representing planktonic freshwater bacteria
Int J Syst Evol Microbiol 59, 112 (2009); https://doi.org/10.1099/ijs.0.001743-0
/content/journal/ijsem/10.1099/ijs.0.001743-0
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Growth of ' Limnoluna rubra' strain MWH-EgelM2-3 and an accompanying non-actinobacterial strain on NSY agar plates. The colonies of the candidate species are red-pigmented and those of the other strain are unpigmented. The plated liquid culture contained about 10 cells ml and was dominated by the actinobacterial strain (>90 % of cells). The photo shows a section of a plate that received an inoculum of about 10 cells. The strain of the candidate species grows well when in direct contact with the non-pigmented colonies; however, the size of the actinobacterial colonies decreases with distance from the non-pigmented colonies and, beyond a crucial distance from these colonies, no actinobacterial growth occurs.

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Neighbour-joining tree based on almost full-length 16S rRNA gene sequences ( positions 47–1465). The tree reconstructs the phylogenetic relationships between the novel candidate species and environmental sequences of uncultured actinobacteria. [PDF](510 KB)

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[PDF file of Supplementary Tables S1 and S2](68 KB)

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