1887

Abstract

A taxonomic study was carried out on strain P24, which was isolated from a polycyclic aromatic hydrocarbon-degrading consortium, enriched from a deep-seawater sample collected from the Indian Ocean. The isolate was Gram-negative, rod-shaped, motile by means of a polar flagellum, moderately halophilic and capable of reducing nitrate to nitrite. Growth was observed at salinities of 0–9 % and at temperatures of 10–42 °C. The strain was unable to degrade Tween 80 or gelatin. The dominant fatty acids were C (15.2 % of the total), C (10.3 %), C 7 (52.0 %), C 2-OH (4.7 %) and C 8 cyclo (4.7 %). The G+C content of the chromosomal DNA was 64.8 mol%. 16S rRNA gene sequence comparisons showed that strain P24 was related most closely to CL-GR58 (92.7 % similarity); levels of similarity between strain P24 and type strains of recognized species in the family were all less than 90.8 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain P24 formed a distinct evolutionary lineage within the family . Strain P24 could be distinguished from phylogenetically related genera based on differences in several phenotypic properties. On the basis of the phenotypic and phylogenetic data presented, strain P24 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is P24 (=CCTCC AB 208226=LMG 24626=MCCC 1A02083).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.004341-0
2009-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/7/1733.html?itemId=/content/journal/ijsem/10.1099/ijs.0.004341-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors)(1995).Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley.
  2. Biebl, H., Allgaier, M., Tindall, B. J., Koblizek, M., Lünsdorf, H., Pukall, R. & Wagner-Döbler, I.(2005).Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. Int J Syst Evol Microbiol 55, 1089–1096.[CrossRef] [Google Scholar]
  3. Dong, X.-Z. & Cai, M.-Y.(2001).Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation).
  4. Ivanova, E. P., Kiprianova, E. A., Mikhailov, V. V., Levanova, G. F., Garagulya, A. D., Gorshkova, N. M., Vysotskii, M. V., Nicolau, D. V., Yumoto, N. & other authors(1998). Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48, 247–256.[CrossRef] [Google Scholar]
  5. Liu, C. & Shao, Z.(2005).Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55, 1181–1186.[CrossRef] [Google Scholar]
  6. Mesbah, M. & Whitman, W. B.(1989). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef] [Google Scholar]
  7. Navarro, J. B., Moser, D. P., Flores, A., Ross, C., Rosen, M. R., Dong, H., Zhang, G. & Hedlund, B. P.(2009). Bacterial succession within an ephemeral hypereutrophic Mojave Desert playa Lake. Microb Ecol 57, 307–320.[CrossRef] [Google Scholar]
  8. Rzhetsky, A. & Nei, M.(1992). A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9, 945–967. [Google Scholar]
  9. Rzhetsky, A. & Nei, M.(1993). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10, 1073–1095. [Google Scholar]
  10. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  11. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  12. Sasser, M.(1997).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  13. Shieh, W. Y., Chen, Y.-W., Chaw, S.-M. & Chiu, H.-H.(2003).Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53, 479–484.[CrossRef] [Google Scholar]
  14. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  15. Zhang, G. I., Hwang, C. Y. & Cho, B. C.(2008).Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. Int J Syst Evol Microbiol 58, 479–485.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.004341-0
Loading
/content/journal/ijsem/10.1099/ijs.0.004341-0
Loading

Data & Media loading...

Supplements

[PDF](727 KB)

PDF

[PDF](43 KB)

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error