1887

Abstract

We previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). Of the five strains, four were identified as members of existing species; however, sequence analysis of the 16S rRNA gene revealed that the fifth isolate, DI70, showed approximately 97 % identity (1325/1366 bp) with the type strain of . Therefore, we considered the possibility that DI70 represents a novel species of the genus . Cells of strain DI70 were Gram-stain-positive, catalase-negative, non-spore-forming, non-motile rods. In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70 formed a subcluster in the phylogenetic group and was closely related to , and . However, analysis of DNA–DNA relatedness showed that DI70 was genetically distinct from its phylogenetic relatives. The isolate also exhibited distinct biochemical and physiological characteristics when compared with its phylogenetic relatives. It required anaerobic conditions for growth on agar medium. The results indicate that isolate DI70 indeed represents a novel species of the genus , for which we propose the name sp. nov. The type strain is DI70 (=JCM 14600 =DSM 19284).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.009290-0
2010-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/1/109.html?itemId=/content/journal/ijsem/10.1099/ijs.0.009290-0&mimeType=html&fmt=ahah

References

  1. Al Jassim R. A. M., Scott P. T., Trebbin A. L., Trott D., Pollitt C. C. 2005; The genetic diversity of lactic acid producing bacteria in the equine gastrointestinal tract. FEMS Microbiol Lett 248:75–81 [CrossRef]
    [Google Scholar]
  2. Bailey S. R., Baillon M.-L., Rycroft A. N., Harris P. A., Elliott J. 2003; Identification of equine cecal bacteria producing amines in an in vitro model of carbohydrate overload. Appl Environ Microbiol 69:2087–2093 [CrossRef]
    [Google Scholar]
  3. Cato E. P., Moore W. E. C., Johnson J. L. 1983; Synonymy of strains of “ Lactobacillus acidophilus ” group A2 (Johnson et al. 1980) with the type strain of Lactobacillus crispatus (Brygoo and Aladame 1953) Moore and Holdman 1970. Int J Syst Bacteriol 33:426–428 [CrossRef]
    [Google Scholar]
  4. Cavalli-Sforza L. L., Edwards A. W. F. 1967; Phylogenetic analysis. Models and estimation procedures. Am J Hum Genet 19:233–257
    [Google Scholar]
  5. Endo A., Okada S., Morita H. 2007; Molecular profiling of Lactobacillus , Streptococcus , and Bifidobacterium species in feces of active racehorses. J Gen Appl Microbiol 53:191–200 [CrossRef]
    [Google Scholar]
  6. Endo A., Roos S., Satoh E., Morita H., Okada S. 2008; Lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses. Int J Syst Evol Microbiol 58:914–918 [CrossRef]
    [Google Scholar]
  7. Ezaki T., Saidi S. M., Liu S.-L., Hashimoto Y., Yamamoto H., Yabuuchi E. 1990; Rapid procedure to determine the DNA base composition from small amounts of Gram-positive bacteria. FEMS Microbiol Lett 67:127–130 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Gasser F., Mandel M., Rogosa M. 1970; Lactobacillus jensenii sp. nov., a new representative of the subgenus Thermobacterium . J Gen Microbiol 62:219–222 [CrossRef]
    [Google Scholar]
  10. Hammes W. P., Hertel C. 2006; The genera Lactobacillus and Carnobacterium . In The Prokaryotes , 3rd edn. vol 4 pp 320–403 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  11. Kandler O., Weiss N. 1986; Genus Lactobacillus Beijerinck 1901, 212AL . In Bergey's Manual of Systematic Bacteriology vol 2 pp 1209–1234 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  13. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  15. Mitsuoka T. 1969; Vergleichende Untersuchungen über die Lactobazillen aus den Faeces von Menschen, Schweinen und Hühnern. Zentralbl Bakteriol [Orig] 210:32–51 (in German
    [Google Scholar]
  16. Morita H., Kuwahara T., Ohshima K., Sasamoto H., Itoh K., Hattori M., Hayashi T., Takami H. 2007a; An improved DNA isolation method for metagenomic analysis of the microbial flora of the human intestine. Microbes Environ 22:214–222 [CrossRef]
    [Google Scholar]
  17. Morita H., Shiratori M., Murakami M., Takami H., Kato Y., Endo A., Nakajima F., Takagi M., Akita H. other authors 2007b; Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds. Int J Syst Evol Microbiol 57:2836–2839 [CrossRef]
    [Google Scholar]
  18. Morita H., Nakano A., Shimazu M., Toh H., Nakajima F., Nagayama M., Hisamatsu S., Kato Y., Takagi M. other authors 2009; Lactobacillus hayakitensis , L. equigenerosi and L. equi , predominant lactobacilli in the intestinal flora of healthy thoroughbreds. Anim Sci J 80:339–346 [CrossRef]
    [Google Scholar]
  19. Morotomi M., Yuki N., Kado Y., Kushiro A., Shimazaki T., Watanabe K., Yuyama T. 2002; Lactobacillus equi sp. nov., a predominant intestinal Lactobacillus species of the horse isolated from faeces of healthy horses. Int J Syst Evol Microbiol 52:211–214
    [Google Scholar]
  20. Rogosa M., Hansen P. A. 1971; Nomenclatural considerations of certain species of Lactobacillus Beijerinck. Request for an Opinion. Int J Syst Bacteriol 21:177–186 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Schleifer K. H., Ludwig W. 1996; Phylogeny of the genus Lactobacillus and related genera. Syst Appl Microbiol 18:461–467
    [Google Scholar]
  23. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.009290-0
Loading
/content/journal/ijsem/10.1099/ijs.0.009290-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error