1887

Abstract

A novel bacterium, TRM 0175, belonging to the genus , was isolated from a soil sample taken from a salt lake in Xinjiang Province, north-west China. The isolate was Gram-negative, aerobic, rod-shaped and motile by means of peritrichous flagella. It was catalase-positive and oxidase-negative. Growth occurred at NaCl concentrations of 0–20 % (optimum at 10–13 %), at 15–50 °C (optimum at 37 °C) and at pH 6.0–9.0 (optimum at pH 7.0). Metabolism was respiratory with oxygen as terminal electron acceptor. Acid was produced from -ribose, - and -arabinose, -xylose, -galactose, -mannose, -rhamnose, cellobiose, maltose, trehalose and - and -fucose and was produced weakly from aesculin. The predominant ubiquinone was Q-9. The major fatty acids were C 7 and C cyclo 8. The GC content of the genomic DNA was 60.0 mol%. The affiliation of strain TRM 0175 with the genus was confirmed by 16S rRNA gene sequence comparisons. The most closely related species was ; 16S rRNA gene sequence similarity between FP35 and strain TRM 0175 was 95.3 %. Phenotypically, some characteristics of TRM 0175 differed from those of . On the basis of data from this polyphasic study, strain TRM 0175 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is TRM 0175 (=CCTCC AB 208329 =KCTC 22608).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.011593-0
2010-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/2/349.html?itemId=/content/journal/ijsem/10.1099/ijs.0.011593-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Arahal D. R., Castillo A. M., Ludwig W., Schleifer K. H., Ventosa A. 2002; Proposal of Cobetia marina gen. nov., comb. nov. within the family Halomonadaceae , to include the species Halomonas marina . Syst Appl Microbiol 25:207–211 [CrossRef]
    [Google Scholar]
  3. Arahal D. R., Vreeland R. H., Litchfield C. D., Mormile M. R., Tindall B. J., Oren A., Bejar V., Quesada E., Ventosa A. 2007; Recommended minimal standards for describing new taxa of the family Halomonadaceae . Int J Syst Evol Microbiol 57:2436–2446 [CrossRef]
    [Google Scholar]
  4. Ben Ali Gam Z., Abdelkafi S., Casalot L., Tholozan J. L., Oueslati R., Labat M. 2007; Modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, moderately halophilic bacterium isolated from an oilfield-water injection sample, and emended description of the family Halomonadaceae Franzmann et al. 1989 emend. Dobson and Franzmann 1996 emend; Ntougias et al. 2007. Int J Syst Evol Microbiol 57:2307–2313 [CrossRef]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  6. Dobson S. J., Franzmann P. D. 1996; Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas , and placement of the genus Zymobacter in the family Halomonadaceae . Int J Syst Bacteriol 46:550–558 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  10. Franzmann P. D., Wehmeyer U., Stackebrandt E. 1988; Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya . Syst Appl Microbiol 11:16–19 [CrossRef]
    [Google Scholar]
  11. Garriga M., Ehrmann M. A., Arnau J., Hugas M., Vogel R. F. 1998; Carnimonas nigrificans gen. nov., sp. nov. a bacterial causative agent for black spot formation on cured meat products. Int J Syst Bacteriol 48:677–686 [CrossRef]
    [Google Scholar]
  12. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov. a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [CrossRef]
    [Google Scholar]
  13. Hezayen F. F., Tindall B. J., Steinbüchel A., Rehm B. H. A. 2002; Characterization of a novel halophilic archaeon, Halobiforma haloterrestris gen. nov., sp. nov. and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. Int J Syst Evol Microbiol 52:2271–2280 [CrossRef]
    [Google Scholar]
  14. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  15. Martínez-Cánovas M. J., Béjar V., Martínez-Checa F., Quesada E. 2004; Halomonas anticariensis sp. nov., from Fuente de Piedra, a saline-wetland wildfowl reserve in Málaga, southern Spain. Int J Syst Evol Microbiol 54:1329–1332 [CrossRef]
    [Google Scholar]
  16. Mata J. A., Martínez-Cánovas M. J., Quesada E., Béjar V. 2002; A detailed phenotypic characterization of the type strain of Halomonas species. Syst Appl Microbiol 25:360–375 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  18. Ntougias S., Zervakis G. I., Fasseas C. 2007; Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996 Int J Syst Evol Microbiol 57:1975–1983 [CrossRef]
    [Google Scholar]
  19. Okamoto T., Taguchi H., Nakamura K., Ikenaga H., Kuraishi H., Yamasato K. 1993; Zymobacter palmae gen. nov., sp. nov. a new ethanol-fermenting peritrichous bacterium isolated from palm sap. Arch Microbiol 160:333–337
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  22. Ventosa A., Gutierrez M. C., Garcia M. T., Ruiz-Berraquero F. 1989; Classification of “ Chromobacterium marismortui ” in a new genus, Chromohalobacter gen. nov., as Chromohalobacter marismortui comb. , nov., nom. rev. Int J Syst Bacteriol 39382–386 [CrossRef]
    [Google Scholar]
  23. Vreeland R. H., Litchfield C. D., Martin E. L., Elliot E. 1980; Halomonas elongata , a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30:485–495 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.011593-0
Loading
/content/journal/ijsem/10.1099/ijs.0.011593-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error