1887

Abstract

A Gram-negative, heterotrophic, aerobic, non-endospore-forming, peritrichously flagellated and motile bacterial strain, designated BH1, was isolated from samples of rusticles, which are formed in part by a consortium of micro-organisms, collected from the RMS wreck site. The strain grew optimally at 30–37 °C, pH 7.0–7.5 and in the presence of 2–8 % (w/v) NaCl. We carried out a polyphasic taxonomic study in order to characterize the strain in detail. Phylogenetic analyses based on 16S rRNA gene sequence comparison indicated that strain BH1 clustered within the branch consisting of species of The most closely related type strains were (98.6 % 16S rRNA sequence similarity), (98.4 %), (98.3 %) and (97.5 %). Other closely related species were (96.5 % sequence similarity), (96.3 %), (96.3 %), (96.3 %) and (96.2 %). The major fatty acids of strain BH1 were C 7 (36.3 %), C (18.4 %) and C cyclo 8 (17.9 %). The DNA G+C content was 60.0 mol% ( ). Ubiquinone 9 (Q-9) was the major lipoquinone. The phenotypic features, fatty acid profile and DNA G+C content further supported the placement of strain BH1 in the genus . DNA–DNA hybridization values between strain BH1 and CECT 5815, DSM 3051, DSM 15516 and CECT 5817 were 19, 17, 30 and 29 %, respectively, supporting the differential taxonomic status of BH1. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain BH1 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is BH1 (=ATCC BAA-1257 =CECT 7585 =JCM 16411 =LMG 25388).

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2010-12-01
2024-03-28
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