1887

Abstract

A strain (KRED) that formed endospores and produced the pigment prodigiosin was isolated from activated sludge. The presence of spores in cells of strain KRED was evident upon electron microscopy examination, heat treatment and the detection of dipicolinic acid in the cells. Biochemical characteristics, and 16S rDNA sequence and DNA–DNA homology data identified strain KRED as . The major respiratory quinone of strain KRED was found to be ubiquinone Q-8. The formation of endospores by Gram-negative bacteria has not been observed previously, and has never been reported in any species of . Here, it is shown that strain KRED (JCM 11315=CIP 107489) represents a novel subspecies of , for which the name subsp. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02158-0
2003-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/1/ijs530253.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02158-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Brenner D. J. 1984; Family I. Enterobacteriaceae Rahn 1937, Nom. Fam. Cons. Opin. 15, Jud. Comm. 1958, 73; Ewing, Farmer and Brenner 1980, 674; Judicial Commission 1981, 104. In Bergey's Manual of Systematic Bacteriology vol 1 pp 408–420Edited by Krieg N. R. , Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316–354
    [Google Scholar]
  4. Doi Y., Lee B.-S., Iriye R., Tabata S., Tateishi K. 1998; Dominantly growing bacteria in malodorless domestic sewage treatment tanks and their biochemical characteristics. J Antibact Antifungal Agents (Japan) 26:53–63
    [Google Scholar]
  5. Edward R. L. 1997; Prokaryotic diversity: form, ecophysiology, and habitat. In Manual of Environmental Microbiology pp 14–24Edited by Christon J. H., Guy R. K., Michael J. M., Linda D. S., Michael V. W. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  7. Gerber N. N. 1975; Prodigiosin-like pigments. C R C Crit Rev Microbiol 3:469–485 [CrossRef]
    [Google Scholar]
  8. Grimont F., Grimont P. A. D. 1992; The genus Serratia . In The Prokaryotes vol 3, 2nd edn. pp 2822–2848Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer-Verlag;
    [Google Scholar]
  9. Grimont P. A. D., Grimont F. 1984; Genus VIII. Serratia Bizio 1823, 288AL. In Bergey's Manual of Systematic Bacteriology vol 1 pp 477–484Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Hearn W. R., Elson M. K., Williams R. H., Medina-Castro J. 1970; Prodigiosin [5-(2-pyrryl)-2,2)-dipyrrylmethene] and some substituted prodigiosenes. J Org Chem 35:142–145 [CrossRef]
    [Google Scholar]
  11. Hiraishi A. 1999; Isoprenoid quinones as biomarkers of microbial populations in the environment. J Biosci Bioeng 88:449–460 [CrossRef]
    [Google Scholar]
  12. Janssen F. W., Lund A. J., Anderson L. F. 1958; Colorimetric assay for dipicolinic acid in bacterial spores. Science 127:26–27 [CrossRef]
    [Google Scholar]
  13. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1996; The Ribosomal Database Project (RDP. Nucleic Acids Res 24:82–85 [CrossRef]
    [Google Scholar]
  14. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  15. Pizzimenti F. C., Nostro A., Marino A., Villari A., Verzera A., Trozzi A., Alonzo V. 1999; Fatty acid profiles in pigmented and non-pigmented strains of S. marcescens. New Microbiol 2291–98
    [Google Scholar]
  16. Powell J. F. 1953; Isolation of dipicolinic acid (pyridine-2,6-dicarboxylic acid) from spores of Bacillus megaterium. Biochem J 54205
  17. Ralph A., Slepecky H., Hemphill E. 1992; The genus Bacillus – nonmedical. In The Prokaryotes vol 2, 2nd edn. p 1673Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer-Verlag;
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–651Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Sneath P. H A. 1989; Endospore-forming Gram-positive rods and cocci. In Bergey's Manual of Systematic Bacteriology vol 2 pp 1104–1105Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  22. Van de Peer Y., De Wachter R. 1997; Construction of evolutionary distance trees with treecon for Windows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 13:227–230
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02158-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02158-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error