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Volume 61, Issue 9

sp. nov., isolated from deep-sea water of the Indian Ocean Free

Abstract

A taxonomic study was carried out on a novel bacterial strain, designated B108, which was isolated from a polycyclic aromatic hydrocarbon (PAH)-degrading consortium, enriched from deep-sea water of the Indian Ocean. The isolate was Gram-reaction-negative, rod-shaped and non-motile. Growth of strain B108 was observed in 1–15 % (w/v) NaCl and at 10–39 °C and it was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain B108 was most closely related to HJ50 (97.1 % sequence similarity), followed by 81-2 (96.6 %) and SMK-122 (95.2 %); other species shared <95.0 % sequence similarity. DNA–DNA hybridization tests showed that strain B108 had a low DNA–DNA relatedness to HJ50 and . 81-2 (48±4 % and 44±5 %, respectively). The predominant fatty acids were C, C 2-OH, summed feature 8 (Cω7/ω6) and Cω8 cyclo, which accounted for 84.2 % of the total cellular fatty acids. The G+C content of the chromosomal DNA was 63.6 mol%. The major respiratory quinone was ubiquinone 10 (Q10). Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and some unidentified compounds were detected. These characteristics were in good agreement with those of members of the genus . The gene was also detected. According to its morphology, physiology, fatty acid composition and phylogenetic position based on 16S rRNA sequence data, the novel strain most appropriately belongs to the genus but can be readily distinguished from known species of this genus. Therefore, strain B108 represents a novel species, of the genus , for which the name sp. nov. is proposed. The type strain is B108 ( = 2PR52-14  = CCTCC AB 208233  = LMG 24622  = MCCC 1A01227).

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Funding
This study was supported by the:
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award 2005DKA21209)
  • National Natural Science Foundation of China (Award 40906083)
  • Scientific Research Foundation of Third Institute of Oceanography, State Oceanic Administration (Award 2009065)
© 2011 IUMS
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2011-09-01
2024-04-20
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Roseovarius indicus sp. nov., isolated from deep-sea water of the Indian Ocean
Int J Syst Evol Microbiol 61, 2040 (2011); https://doi.org/10.1099/ijs.0.023168-0
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